GPT Antibody (E-4): sc-271371
- GPT Antibody (E-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-33 at the N-terminus of GPT of mouse origin
- recommended for detection of GPT of mouse origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GPT Antibody (E-4) for WB applications. These reagents are now offered in bundles with GPT Antibody (E-4) (see ordering information below).
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GPT Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects glutamate pyruvate transaminase (GPT) in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GPT antibody (E-4) recognizes GPT, which plays a crucial role in amino acid metabolism by catalyzing the reversible transamination between alanine and 2-oxoglutarate to produce pyruvate and glutamate. This enzymatic activity is vital for maintaining the balance of amino acids and energy production in the body, particularly in tissues with high metabolic demands such as the liver, kidney, and heart. GPT (E-4) antibody enables detection within the cytoplasm of these cells, allowing for efficient substrate access and rapid metabolic responses to physiological changes. Additionally, GPT serves as a biomarker for liver integrity, making GPT (E-4) monoclonal antibody an important tool in clinical diagnostics for hepatocellular damage. With high specificity and sensitivity, GPT monoclonal antibody (E-4) proves essential for researchers studying metabolic pathways and liver function in mouse models.
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GPT Antibody (E-4) References:
- AFB(1) -induced mutagenesis of the gpt gene in AS52 cells. | Wattanawaraporn, R., et al. 2012. Environ Mol Mutagen. 53: 567-73. PMID: 22733615
- In vivo genotoxicity of 1-methylnaphthalene from comprehensive toxicity studies with B6C3F1 gpt delta mice. | Jin, M., et al. 2012. J Toxicol Sci. 37: 711-21. PMID: 22863852
- In vivo cII, gpt, and Spi⁻ gene mutation assays in transgenic mice and rats. | Manjanatha, MG., et al. 2013. Methods Mol Biol. 1044: 97-119. PMID: 23896873
- Tissue-specific and time-dependent clonal expansion of ENU-induced mutant cells in gpt delta mice. | Nakayama, T., et al. 2017. Environ Mol Mutagen. 58: 592-606. PMID: 28921690
- Modulation of N-Methyl-N-nitrosourea Mutagenesis in Mouse Embryo Fibroblasts Derived from the gpt Delta Mouse by an Inhibitor of the O6-Methylguanine Methyltransferase, MGMT. | Thongararm, P., et al. 2020. Chem Res Toxicol. 33: 625-633. PMID: 31841318
- Past, Present and Future Directions of gpt delta Rodent Gene Mutation Assays. | Nohmi, T. 2016. Food Saf (Tokyo). 4: 1-13. PMID: 32231899
- Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt delta. | Nohmi, T. and Watanabe, M. 2021. Genes Environ. 43: 38. PMID: 34526143
- The effect of monosodium glutamate on GHD, GOT, and GPT levels in mice. | Arthur, RD., et al. 1973. Proc Soc Exp Biol Med. 144: 34-7. PMID: 4771579
- Chromosomal location of soluble glutamic-pyruvic transaminase-1 (Gpt-1) in the mouse. | Eicher, EM. and Womack, JE. 1977. Biochem Genet. 15: 1-8. PMID: 849243
- Chromosomal and regional localization of the loci for IGKC, IGGC, ALDB, HOXB, GPT, and PRNP in the American mink (Mustela vison): comparisons with human and mouse. | Khlebodarova, TM., et al. 1995. Mamm Genome. 6: 705-9. PMID: 8563168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPT Antibody (E-4) | sc-271371 | 200 µg/ml | $322.00 | |||
GPT Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-540232 | 200 µg Ab; 10 µg BP | $361.00 | |||
GPT Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-541988 | 200 µg Ab; 20 µg BP | $361.00 | |||
GPT (E-4) Neutralizing Peptide | sc-271371 P | 100 µg/0.5 ml | $69.00 |