Date published: 2025-9-6
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Glucose Transporter Glut2 Antibody (C-10) - Western Blotting - Image 386258
Glut2 抗体 (C-10): sc-518022. 非转染: sc-117752 (A) 和小鼠转染 Glut2: sc-120518 (B) 全细胞裂解液中 Glut2 表达的 Western 印迹分析.
Glucose Transporter Glut2 Antibody (C-10) - Western Blotting - Image 386258
Glut2 抗体 (C-10): sc-518022. 非转染: sc-117752 (A) 和小鼠转染 Glut2: sc-120518 (B) 全细胞裂解液中 Glut2 表达的 Western 印迹分析.
Glut2 抗体 (C-10): sc-518022. 非转染: sc-117752 (A) 和小鼠转染 Glut2: sc-120518 (B) 全细胞裂解液中 Glut2 表达的 Western 印迹分析.

Glucose Transporter Glut2 抗体 (C-10): sc-518022

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寫評論提問

说明书
  • Glucose Transporter Glut2 抗体 C-10 是小鼠单克隆 IgG1 κ，Glucose Transporter Glut2抗体, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸32-98
  • Glucose Transporter Glut2 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Glut2
  • 抗Glucose Transporter Glut2抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取Glucose Transporter Glut2 (C-10): sc-518022的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是Glucose Transporter Glut2 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Glucose Transporter Glut2 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Glut抗体，包括Glut1, Glut10, Glut2, Glut3, Glut4, Glut5和Glut6

Glut2抗体（C-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Glut2蛋白。抗 -Glut2抗体（C-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Glut2在葡萄糖稳态中起着至关重要的作用，主要作为低亲和力葡萄糖转运蛋白，根据葡萄糖浓度的变化促进葡萄糖跨细胞膜转运。Glut2在胰腺β细胞中的表达尤为重要，它参与葡萄糖感应和胰岛素分泌，此外在肝细胞和肠上皮细胞的基底膜中也有表达。Glut2在高血糖条件下有效运输葡萄糖的能力对于维持能量平衡和代谢调节至关重要。Glut2独特的低亲和性使其能够充当葡萄糖传感器，为胰腺提供有关血糖水平的重要反馈，这对于正常的胰岛素分泌机制至关重要。Glut2是葡萄糖代谢调节的关键参与者，也是代谢紊乱治疗干预的潜在目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glucose Transporter Glut2 参考文献:

  1. GLUT2、葡萄糖感应和葡萄糖稳态。  |  Thorens, B. 2015. Diabetologia. 58: 221-32. PMID: 25421524
  2. PAR2通过GLUT2和Akt的干扰，促进NAFLD中葡萄糖摄取受损和胰岛素抵抗。  |  Shearer, AM., et al. 2022. Hepatology. 76: 1778-1793. PMID: 35603482
  3. 肾近端小管GLUT2--远不止表面现象。  |  Ahmad, M., et al. 2022. Cells. 12: PMID: 36611887
  4. CD8+ T细胞中的GLUT2、葡萄糖和氧传感。  |  Tong, X. 2023. Nat Metab. 5: 1850-1851. PMID: 37884693
  5. 小鼠服用小檗碱后,通过促进PPARγ-FGF21-GLUT2调节胰岛素敏感性和葡萄糖/脂质平衡,从而预防2型糖尿病。  |  Chen, Y., et al. 2023. Biochem Pharmacol. 218: 115928. PMID: 37979703
  6. 3T3-L1 脂肪细胞中 GLUT-4 靶向的分子调控。  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
  7. 培养的大鼠脂肪细胞中葡萄糖转运体的调控：胰岛素和地塞米松通过启动子激活对 GLUT4 基因表达的协同作用。  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
  8. 大鼠前脑整体缺血后，神经元特异性葡萄糖转运体 GLUT3 在海马中的免疫反应性逐渐丧失。  |  McCall, AL., et al. 1995. Brain Res. 670: 29-38. PMID: 7719721
  9. 洞穴素在脂肪细胞中的表达和分区：与 GLUT4 的协调调控和结构分离。  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
  10. 下丘脑 GLUT 4 的表达：葡萄糖和胰岛素感应机制？  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
  11. 促进葡萄糖转运体  |  Mueckler, M. 1994. Eur J Biochem. 219: 713-25. PMID: 8112322
  12. 葡萄糖转运体在肠道, 肾脏和肝脏葡萄糖通量调节中的作用。  |  Thorens, B. 1996. Am J Physiol. 270: G541-53. PMID: 8928783

订购信息

产品名称产品编号规格价格数量收藏夹

Glucose Transporter Glut2 抗体 (C-10)

sc-518022
200 µg/ml
$316.00

Glucose Transporter Glut2 (C-10): m-IgG1 BP-HRP 套装

sc-531816
200 µg Ab; 20 µg BP
$354.00

Glucose Transporter Glut2 抗体 (C-10) AC

sc-518022 AC
500 µg/ml, 25% agarose
$416.00

Glucose Transporter Glut2 抗体 (C-10) HRP

sc-518022 HRP
200 µg/ml
$316.00

Glucose Transporter Glut2 抗体 (C-10) FITC

sc-518022 FITC
200 µg/ml
$330.00

Glucose Transporter Glut2 抗体 (C-10) PE

sc-518022 PE
200 µg/ml
$343.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 488

sc-518022 AF488
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 546

sc-518022 AF546
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 594

sc-518022 AF594
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 647

sc-518022 AF647
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 680

sc-518022 AF680
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) Alexa Fluor® 790

sc-518022 AF790
200 µg/ml
$357.00

Glucose Transporter Glut2 抗体 (C-10) B

sc-518022 B
200 µg/ml
$320.00

Hi, I got bands from total protein lysates of mouse liver. Very dark bands appeared at 50~55 kDa, and less dark bands appeared slightly below. Why are there two bands? Which size band should I trust? Mouse IgG (Cell Signaling, 7076S) was used as 2nd antib

Asked by: ChoWon
Thank you for your question. For assistance please email technical service at scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-09-09
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Rated 5 out of 5 by from Antibody specifically detects mouse Glut2Antibody shows a strong 57 KDa Western Blot band with transfected lysate sc-120518, that over-expresses mouse Glucose Transporter Glut2 (middle lane). Transfected Lysate's NCBI Reference Sequence: BC034675.1 -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Good antibodyIt is a good antibody for WB. I tried it on isolated human cells [1:1000] and it works fine
Date published: 2021-02-03
Rated 4 out of 5 by from Good Signal in both human and bovine samplesThe product has been tested in western blot against samples derived from human plasma, bovine plasma and on HepG2 whole-cell extracts. Tests were run at 1:1000 dilution in PBS + 0.05% tween-20 + 1% fat-free milk. The molecular weight of the protein has been described to be between 50 and 60 kDa in human. Bovine variant is expected to have a similar weight as well, according to in-silico predictions. In all the samples, we detected a band slightly above 50 kDa that should correspond to the protein of interest, in accordance with product datasheet. We could detect a very strong signal in plasma samples (both human and bovine). A smaller but neat signal was also present in HepG2 lysates.
Date published: 2020-10-09
Rated 3 out of 5 by from Detection by IHC, not Western, in rat INS-1 cellsGood DAB staining in IHC-P rat INS-1 xenograft tumors, but no Western detection in lysates from tumors or cultured cells.
Date published: 2019-05-22
Rated 3 out of 5 by from Noch nicht ganz zufriedenWir haben GLUT 2 und GLUT 3 mit einer Doppelfärbung mit Vimentin im Säugergehirn mit Fluoreszensfarbstoffen gefärbt. Während GLUT 3 (Probengröße) gute Ergebnisse geliefert hat , sind wir mit GLUT 2 noch nicht ganz zufrieden, werden aber weiterhin den Antikörper testen.
Date published: 2018-12-07
Rated 4 out of 5 by from Good for IF (P)It worked for immunofluorescence staining in paraffin sections of human and murine intestine.
Date published: 2018-03-28
Rated 1 out of 5 by from Poor for western blottingI received a trial size of this antibody. In western blotting, it was unable to detect the protein of interest.
Date published: 2018-03-13
Rated 5 out of 5 by from Multiple tissues positiveWe ran this using mouse FFPE liver, intestine and kidney tissues. The results are very clear, low background with dark positive results. I suggest using this for any FFPE mouse study.
Date published: 2018-02-10
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Glucose Transporter Glut2 抗体 (C-10) is rated 3.6 out of 5 by 8.
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