Date published: 2025-9-5

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GKLF/KLF4 抗体 (B-8): sc-393462

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说明书
  • GKLF/KLF4 抗体 B-8 是小鼠单克隆 IgG2b κ,GKLF/KLF4抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的GKLF的N-terminus附近的氨基酸20-41之间
  • GKLF/KLF4 抗体 (B-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 GKLF
  • 抗GKLF/KLF4抗体(B-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-393462 X, 200 µg/0.1 ml)
  • 关于如何获取GKLF/KLF4 (B-8): sc-393462免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是GKLF/KLF4 Antibody (B-8) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与GKLF/KLF4 Antibody (B-8) 打包提供(请参阅下面的订购信息)。

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関連項目

GKLF抗体(B-8)是一种IgG2b κ小鼠单克隆GKLF抗体(也称为KLF4抗体、Kruppel样因子4抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的GKLF蛋白。GKLF抗体(B-8)既可作为非共轭抗GKLF抗体形式,也可作为多种共轭形式的抗GKLF抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Kruppel型锌指转录因子是一个保守的DNA结合蛋白家族,在发育调控中起重要作用。Kruppel锌指转录因子最初在果蝇中被鉴定为分段基因。在哺乳动物中已鉴定的Kruppel样因子包括EKLF、LKLF和GKLF。EKLF主要在红细胞组织中表达,而LKLF的表达仅限于肺。GKLF主要存在于肠道中,并已被证明在生长停滞期间表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GKLF/KLF4 抗体 (B-8) 参考文献:

  1. 通过逆转录病毒转导腺病毒E1A-不朽化的大鼠肾RK3E细胞进行癌基因表达克隆:通过c-MYC和锌指蛋白GKLF转化具有上皮特征的宿主。  |  Foster, KW., et al. 1999. Cell Growth Differ. 10: 423-34. PMID: 10392904
  2. Krüppel样转录因子Zf9和GKLF共同激活人类角蛋白4启动子并发生物理作用。  |  Okano, J., et al. 2000. FEBS Lett. 473: 95-100. PMID: 10802067
  3. Sp1, GKLF 和 CREB-2 的相互作用控制着人类 Pre-α-Inhibitor 基因(ITIH3)的转录。  |  Ruminy, P., et al. 2003. Gene. 315: 133-44. PMID: 14557073
  4. 编码锌指蛋白的人类基因的特征和图谱。  |  Bray, P., et al. 1991. Proc Natl Acad Sci U S A. 88: 9563-7. PMID: 1946370
  5. DNA 结合抑制因子 3(Id3)与富含肠道的克鲁珀尔样因子(GKLF)和 p53 的相互作用可调节血管平滑肌细胞的增殖。  |  Wassmann, K., et al. 2010. Mol Cell Biochem. 333: 33-9. PMID: 19618124
  6. 编码锌指结构的小鼠多基因家族的特征。  |  Chavrier, P., et al. 1988. Mol Cell Biol. 8: 1319-26. PMID: 2452975
  7. 人类基因 GLI-Kruppel 家族。  |  Ruppert, JM., et al. 1988. Mol Cell Biol. 8: 3104-13. PMID: 2850480
  8. 包含果蝇分节基因 Krüppel 编码的指状蛋白结构元素的核蛋白保守家族。  |  Schuh, R., et al. 1986. Cell. 47: 1025-32. PMID: 3096579
  9. 在杆状病毒表达系统中产生的果蝇 Krüppel 基因产物是一种能与 DNA 结合的核磷蛋白。  |  Ollo, R. and Maniatis, T. 1987. Proc Natl Acad Sci U S A. 84: 5700-4. PMID: 3112773
  10. 分离出编码与红细胞 Krüppel 样因子同源的功能性锌指蛋白的基因:确定了一个新的多基因家族。  |  Anderson, KP., et al. 1995. Mol Cell Biol. 15: 5957-65. PMID: 7565748
  11. 鉴定和表征一种在生长停滞期间表达的肠道富集克鲁珀尔样因子的编码基因。  |  Shields, JM., et al. 1996. J Biol Chem. 271: 20009-17. PMID: 8702718
  12. 人类红细胞克鲁佩尔样因子(EKLF)的分离, 基因组结构和表达。  |  Bieker, JJ. 1996. DNA Cell Biol. 15: 347-52. PMID: 8924208

订购信息

产品名称产品编号规格价格数量收藏夹

GKLF/KLF4 抗体 (B-8)

sc-393462
200 µg/ml
$316.00

GKLF/KLF4 (B-8): m-IgG Fc BP-HRP 套装

sc-526255
200 µg Ab; 10 µg BP
$354.00

GKLF/KLF4 (B-8): m-IgG2b BP-HRP 套装

sc-549703
200 µg Ab; 10 µg BP
$354.00

GKLF/KLF4 抗体 (B-8) X

sc-393462 X
200 µg/0.1 ml
$316.00

GKLF/KLF4 抗体 (B-8) AC

sc-393462 AC
500 µg/ml, 25% agarose
$416.00

GKLF/KLF4 抗体 (B-8) HRP

sc-393462 HRP
200 µg/ml
$316.00

GKLF/KLF4 抗体 (B-8) FITC

sc-393462 FITC
200 µg/ml
$330.00

GKLF/KLF4 抗体 (B-8) PE

sc-393462 PE
200 µg/ml
$343.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 488

sc-393462 AF488
200 µg/ml
$357.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 546

sc-393462 AF546
200 µg/ml
$357.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 594

sc-393462 AF594
200 µg/ml
$357.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 647

sc-393462 AF647
200 µg/ml
$357.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 680

sc-393462 AF680
200 µg/ml
$357.00

GKLF/KLF4 抗体 (B-8) Alexa Fluor® 790

sc-393462 AF790
200 µg/ml
$357.00

GKLF/KLF4 (B-8) 中和勝肽

sc-393462 P
100 µg/0.5 ml
$68.00

I want to know what is the volume of the Anti-GKLF/EKLF/LKLF/KLF4/1/2 Antibody (F-8) HRP?  Is there any suggest dilution of the antibody?

Asked by: Flor
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-03

Can GKLF (B-8): sc-393462 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from Clean signal in WBIt seems to work well and give a clean signal. I will post the result after silencing as soon as I do it.
Date published: 2025-01-29
Rated 4 out of 5 by from Nice antibodyUsed with 20 ug of K562 protein extract, worked well but some aspecific bands appears during long exposure.
Date published: 2021-04-22
Rated 5 out of 5 by from Works well by WB on mouse colon extractsThis ab worked well by WB on mouse colon extracts (20ug) at a 1/500 dilution. Showed a signal a the right molecular weight after saturation and incubation of the ab in TBST-5% non fat milk and revelation with regular ECL
Date published: 2017-10-27
Rated 5 out of 5 by from Robust signal in WB!This GKLF monoclonal antibody gives a robust and consistent signal in mouse, rat and human whole cell lysates.
Date published: 2016-12-23
Rated 5 out of 5 by from Nice Western Blot data of GKLF expression in nonNice Western Blot data of GKLF expression in non-transfected and human GKLF transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Produced positive Western Blot data of GKLFProduced positive Western Blot data of GKLF expression in non-transfected 293T, mouse GKLF transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-09-30
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GKLF/KLF4 抗体 (B-8) is rated 4.8 out of 5 by 6.
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