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GFP Antibody (1A5) - Immunofluorescence - Image 156467
GFP 抗体 (1A5): sc-101536. COS 细胞免疫荧光染色.
GFP Antibody (1A5) - Immunofluorescence - Image 156467
GFP 抗体 (1A5): sc-101536. COS 细胞免疫荧光染色.
GFP Antibody (1A5) - Western Blotting - Image 156468
GFP 抗体 (1A5): sc-101536. 293T 细胞裂解物中 GFP 表达的 Western 印迹分析.
GFP 抗体 (1A5): sc-101536. COS 细胞免疫荧光染色.

GFP 抗体 (1A5): sc-101536

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说明书
  • GFP 抗体 1A5 是大鼠单克隆 IgG1, 在29篇文献中引用，提供 200 µg/ml
  • 针对重组GFP
  • 推荐用于 GFP and GFP fusion proteins WB, IP, IF 和 ELISA检测
  • 目前，我们还没有完成GFP 抗体 (1A5)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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绿色荧光蛋白（GFP）最初被鉴定为一种参与水母Aequorea victoria生物发光的蛋白质。当在原核细胞中表达时，GFP cDNA会产生荧光产物，不需要外源底物或辅因子，这使得GFP成为监测基因表达和体内蛋白质定位的有用工具。已经开发了几种GFP突变体，包括EGFP，其荧光比野生型GFP更强。它们的激发最大值转移更适合FACS和荧光显微镜以及双标记应用。GFP广泛用于表达载体作为融合蛋白标签，允许表达和监测融合GFP的异源蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GFP 抗体 (1A5) 参考文献:

  1. 分析 21 种新型酿酒酵母开放阅读框的缺失表型和 GFP 融合。  |  Brachat, A., et al. 2000. Yeast. 16: 241-53. PMID: 10649453
  2. 优化 GFP 水平以分析沙门氏菌感染期间的基因表达。  |  Wendland, M. and Bumann, D. 2002. FEBS Lett. 521: 105-8. PMID: 12067736
  3. 通过单克隆抗体耦合亲和层析纯化高纯度和高产量的GFP融合蛋白。  |  Zhuang, R., et al. 2008. Protein Expr Purif. 59: 138-43. PMID: 18342538
  4. 应用GFP标记研究酵母中的朊病毒。  |  Greene, LE., et al. 2009. Protein Pept Lett. 16: 635-41. PMID: 19519522
  5. [利用 GFP 逆转录病毒标记肿瘤细胞和血管内皮细胞]。  |  Xie, K., et al. 2001. Zhongguo Fei Ai Za Zhi. 4: 20-4. PMID: 21040631
  6. 与 tat 肽共同作用可有效增强 HT-29 细胞对 GFP 的吸收和大鼠皮肤的渗透。  |  Lohcharoenkal, W., et al. 2011. J Pharm Sci. 100: 4766-73. PMID: 21681754
  7. 分裂超阳性GFP重组是一种快速、高效且可靠的体内蛋白质相互作用检测方法。  |  Blakeley, BD., et al. 2012. Mol Biosyst. 8: 2036-40. PMID: 22692102
  8. 生物活性血红素-GFP嵌合体的氧依赖性分泌。  |  Chachami, G., et al. 2013. Biochem Biophys Res Commun. 435: 540-5. PMID: 23665013
  9. 无启动子 GFP 盒表达的证据 GFP 是生物技术科学中理想的报告基因吗？  |  Mohammadi, Z., et al. 2019. Res Pharm Sci. 14: 351-358. PMID: 31516512
  10. GFP融合活性hFGF21在烟草叶绿体中的高表达。  |  Wang, Y., et al. 2023. Protein Expr Purif. 208-209: 106271. PMID: 37084839

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产品名称产品编号规格价格数量收藏夹

GFP 抗体 (1A5)

sc-101536
200 µg/ml
$322.00

Does this antibody(GFP Antibody (1A5)) works for venus? If not, do you have any idea which GFP antibody here can recognize Venus?

Asked by: Liu M
Thank you for your question. Please contact our Technical Service Department with the specific protein sequence for Venus, and we can perform sequence analysis of our GFP antibody antigens to see if any of them may also detect this protein. We do not have any data or citations for this GFP antibody specifically mentioning reactivity with Venus.
Answered by: Technical Support
Date published: 2020-03-03

Could you tell me which regions or amino acid of the GFP protein is targeted by this antibody?

Asked by: jfoul78
Thank you for your question. GFP Antibody (1A5) is a rat monoclonal raised against recombinant GFP. Unfortunately, the exact sequence used as an immunogen is not known, nor is the epitope recognized by this specific clone. We do offer a broad variety of GFP antibodies where this information is available. For example, our GFP Antibody (C-2): sc-390394 is specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin. Please contact our Technical Service Department for help in choosing the best antibody for your research needs. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Answered by: Technical Support
Date published: 2017-02-03

I would like to use GFP Antibody (1A5): sc-101536 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101536 is not available directly conjugated, but we have an alternative GFP antibody (sc-9996) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-01-24

I am looking for a GFP antibody that was raised against the amino acid sequence 215-230 (RDHMVL LEFVTAAGIT). Do you have any antibody like this? Thanks!

Asked by: Anette
Thank you for your question. Most of our GFP antibodies were raised against full length recombinant GFP, which includes this portion of the sequence that you have specified. However, the exact epitope recognized by each monoclonal antibody has not been determined. If you would be interested in testing a complimentary sample size of our mouse monoclonal antibody, GFP (B-2): sc-9996, to see if it would work for your purposes, please contact our Technical Service Department. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Answered by: Technical Support
Date published: 2017-01-19
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Rated 1 out of 5 by sharmilee from Did not work in texted tissue samplesI got a free sample and tested it according to th eworking lab protocol.There was no signal in the blot.
Date published: 2018-04-09
Rated 5 out of 5 by Bill28 from Positive WB and IFAntibody is positive for both westren blot and IF staining
Date published: 2017-02-05
Rated 5 out of 5 by TinTin from Reliable antibodyGood choice if you're looking for a reliable GFP antibody to use in WB and IF.
Date published: 2017-01-11
Rated 5 out of 5 by AP from Strong bands at 1:1000 dilutionStrong bands at 1:1000 dilution.
Date published: 2015-09-10
Rated 5 out of 5 by Ayu from Worked well in WBWorked well in WB, no complaints.
Date published: 2015-05-27
Rated 5 out of 5 by HC from Good antibody for western blot analysis ofGood antibody for western blot analysis of GFP expression in 293T cell lysate. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by SF from Work well for IF staining of COS cellsWork well for IF staining of COS cells. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by JT from Generated publishable data on Hela cellsGenerated publishable data on Hela cells with this antibody. -SCBT Publication Review
Date published: 2013-11-23
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GFP 抗体 (1A5) is rated 4.5 out of 5 by 8.
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