Date published: 2025-9-7
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GAPDH Antibody (H-12) - Western Blotting - Image 379466
GAPDH 抗体 (H-12): sc-166574. Hep G2 (A), MCF7 (B), JAR (C), U-251-MG (D), TK-1 (E) 和 C6 (F) 全细胞裂解液中 GAPDH 表达的 Western 印迹分析.
GAPDH 抗体 (H-12): sc-166574. Hep G2 (A), MCF7 (B), JAR (C), U-251-MG (D), TK-1 (E) 和 C6 (F) 全细胞裂解液中 GAPDH 表达的 Western 印迹分析.

GAPDH 抗体 (H-12): sc-166574

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说明书
  • GAPDH抗体(H-12)是小鼠单克隆IgG2a κ, 在277篇文献中引用，规格为200 µg/ml
  • 针对GAPDH的内部区域human起源的肽图
  • GAPDH 抗体 (H-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的GAPDH; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗GAPDH抗体（H-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAPDH (H-12): sc-166574的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 GAPDH 抗体 (H-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAPDH 抗体 (H-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

甘油醛-3-磷酸脱氢酶（GAPDH），也被称为尿嘧啶DNA糖基化酶，在无机磷酸和烟酰胺腺嘌呤二核苷酸（NAD）存在的情况下，催化甘油醛-3-磷酸的可逆氧化磷酸化，这是碳水化合物代谢中产生能量的重要步骤。虽然GAPDH长期以来一直被认为是糖酵解中不可或缺的角色，但GAPDH的其他功能还包括作为尿嘧啶DNA糖基化酶，激活转录，结合RNA并参与核RNA输出，DNA复制和DNA修复。GAPDH在肝癌，肺癌和前列腺癌中的表达上调。GAPDH在细胞凋亡期间易位至细胞核。GAPDH与神经元蛋白复合物与人类神经退行性疾病有关，包括β-淀粉样前体，亨廷顿蛋白和其他三核苷酸重复神经元疾病蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (H-12)

sc-166574
200 µg/ml
$316.00

GAPDH (H-12): m-IgGκ BP-HRP 套装

sc-521661
200 µg Ab, 40 µg BP
$354.00

GAPDH (H-12): m-IgG2a BP-HRP 套装

sc-546102
200 µg Ab; 10 µg BP
$354.00

GAPDH 抗体 (H-12) AC

sc-166574 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 抗体 (H-12) HRP

sc-166574 HRP
200 µg/ml
$316.00

GAPDH 抗体 (H-12) FITC

sc-166574 FITC
200 µg/ml
$330.00

GAPDH 抗体 (H-12) PE

sc-166574 PE
200 µg/ml
$343.00

GAPDH 抗体 (H-12) Alexa Fluor® 488

sc-166574 AF488
200 µg/ml
$357.00

GAPDH 抗体 (H-12) Alexa Fluor® 546

sc-166574 AF546
200 µg/ml
$357.00

GAPDH 抗体 (H-12) Alexa Fluor® 594

sc-166574 AF594
200 µg/ml
$357.00

GAPDH 抗体 (H-12) Alexa Fluor® 647

sc-166574 AF647
200 µg/ml
$357.00

GAPDH 抗体 (H-12) Alexa Fluor® 680

sc-166574 AF680
200 µg/ml
$357.00

GAPDH 抗体 (H-12) Alexa Fluor® 790

sc-166574 AF790
200 µg/ml
$357.00

GAPDH (H-12) 中和勝肽

sc-166574 P
100 µg/0.5 ml
$68.00

What is the recommended dilution for GAPDH Antibody (H-12) in IHC applications?

Asked by: Trav11
We recommend a starting dilution of 1:50 in IHC applications.
Answered by: Tech Service 4
Date published: 2017-01-06

Does this antibody show cross-reactivity with E. coli GAPDH?

Asked by: EGAR
Thank you for your inquiry. The immunogen used to raise our GAPDH antibody sc-166574 shows low homology with the sequence for E.coli GAPDH, so we do not predict it will detect this protein and have no data to support reactivity.
Answered by: Technical Service
Date published: 2016-10-28
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Rated 3 out of 5 by from discreto40ug of protein were loaded in AnyKD precasted gel Electrophoresis was performed in TGS buffer at 100V Immunoblot was performed with Protran Nitrocellulose Membrane at 400mA for 90’ Membranes were incubated 1h in BSA 5% in TBS + Tween 0.1% (TBS-T) GAPDH antibody was diluted 1:1000 in TBS-T and membranes incubated o/n at 4°C Membranes were washed three times with TBS-T for 10’ Secondary antibody anti-mouse HRP conjugated (Bio-Rad 170-6516) was diluted 1:20000 in TBS-T and added to membranes for 1h at RT Signal was detected with ECL (GE GE RPN2209) in the dark room Exposition time 1’ 3’ 15’ In our experimental conditions the antibody worked well and the signal was detected at expected molecular weight
Date published: 2019-07-26
Rated 5 out of 5 by from good bandsThe first time it didn't work as well. But the second time we used a better ECL and it gave as pretty dark bands so it works properly.
Date published: 2019-04-01
Rated 5 out of 5 by from very good antibodyworks on WB in dilution 1:500 in 5%BSA on mouse brain tissue
Date published: 2018-02-15
Rated 3 out of 5 by from work nicelyIt works well on vole's brain tissue extraction for western blot.
Date published: 2017-11-28
Rated 4 out of 5 by from GREAT!GREAT! This antibody worked great on IP for CHIP assay.
Date published: 2017-10-16
Rated 5 out of 5 by from The antibody is perfect for western blot.our lab always uses the antibody in last 5 years. the antibody exhibits excellent specific and reactivites in the WB analysis.
Date published: 2017-07-27
Rated 5 out of 5 by from This project is perfect !This product makes me happy!Because this is the only primary antibody that very useful from my a lab.Other antibodies are really normal ,even no bands on the computer screen sometimes.So the antibody from santa cruz that lets me more confidently when i doing experiment.And the price of these antibodies is not very expensive.I will introduce it to my friends of other teams.
Date published: 2017-07-21
Rated 5 out of 5 by from great antibodyHighly express in mouse liver tissue, the effect is very good
Date published: 2017-06-02
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GAPDH 抗体 (H-12) is rated 4.4 out of 5 by 16.
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