Date published: 2025-9-6

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Gα 11 抗体 (D-6): sc-390382

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说明书
  • G alpha 11 抗体 D-6 是小鼠单克隆 IgG1 κ,Gα 11抗体, 在18篇文献中引用,规格为200 µg/ml
  • 特定于mouse起源的Gα 11的N-terminus内的氨基酸3-39之间的表位图谱
  • G alpha 11 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Gα 11
  • 抗G alpha 11抗体(D-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Gα 11 (D-6): sc-390382免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Gα 11 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Gα 11 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Gα 11抗体(D-6)是一种IgG1 κ型小鼠单克隆G alpha 11抗体(也称为三聚体G蛋白GNA11抗体、G蛋白亚基alpha 11抗体、异源三聚体G蛋白抗体或鸟嘌呤核苷酸结合蛋白alpha 11抗体),能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组化(IHC(P))和酶联免疫吸附测定(ELISA)等方法检测来自小鼠、大鼠和人类的G alpha 11蛋白。Gα 11抗体(D-6)既可以以非偶联的抗G alpha 11抗体形式提供,也可以提供多种偶联形式的抗G alpha 11抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。异源三聚体G蛋白的功能是将信息从细胞表面受体传递到细胞内效应器。非常广泛的受体中的每一个都能特异性地检测细胞外刺激(光子、信息素、气味剂、激素或神经递质),而效应器(例如腺苷酸环化酶)则通过产生一种或多种细胞内信使来发挥作用,但数量较少。在哺乳动物中,G蛋白的α、β和γ多肽分别由至少16、4和7个基因编码。对G蛋白的大部分兴趣都集中在它们的α亚基上,因为这些蛋白能够结合和水解GTP,并且最明显地调节研究得最好的效应器的活性。已经鉴定出四种不同的Ga亚基类别;这些包括Gs、Gi、Gq和Gα 12/13。Gq类包括Gα 15、Gα 14、Gα 11和Gα q,其中Gα 11和Gα q两种在脑和肺中含量丰富,在各种组织中的含量较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gα 11 抗体 (D-6) 参考文献:

  1. G 蛋白 gamma 亚基的选择性组织分布,包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
  2. 异三聚体 GTP 结合蛋白 beta 亚基的多样性:新型 beta 亚基 cDNA 的特征。  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
  3. Gustducin 是一种味觉细胞特异性 G 蛋白,与转导蛋白关系密切。  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
  4. 信号转导中 G 蛋白的多样性。  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
  5. G 蛋白的多样性:脊椎动物和无脊椎动物中都有一类不同的 alpha 亚基。  |  Strathmann, M. and Simon, MI. 1990. Proc Natl Acad Sci U S A. 87: 9113-7. PMID: 2123549
  6. 小鼠肠道上皮细胞稳态维持中Gαq/Gα11信号传导的必要性。  |  Watanabe, N., et al. 2016. Cell Mol Gastroenterol Hepatol. 2: 767-782.e6. PMID: 28174748
  7. 突变 Gα11 基因敲入小鼠可模拟人类遗传性低钙血症,并通过药物抑制剂得到缓解。  |  Roszko, KL., et al. 2017. JCI Insight. 2: e91079. PMID: 28194446
  8. 小鼠的 Gα11 基因突变会导致低钙血症,而钙化疗法可以纠正低钙血症。  |  Gorvin, CM., et al. 2017. JCI Insight. 2: e91103. PMID: 28194447
  9. Gαq/Gα11信号在肠上皮细胞中的作用。  |  Mashima, H., et al. 2018. Biochem Biophys Rep. 13: 93-98. PMID: 29387814
  10. FR900359在葡萄膜黑色素瘤中抑制致癌Gαq和Gα11的作用。  |  Lapadula, D., et al. 2019. Mol Cancer Res. 17: 963-973. PMID: 30567972
  11. 直接靶向癌细胞中的 Gαq 和 Gα11 癌蛋白。  |  Annala, S., et al. 2019. Sci Signal. 12: PMID: 30890659
  12. 与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称产品编号规格价格数量收藏夹

Gα 11 抗体 (D-6)

sc-390382
200 µg/ml
$316.00

Gα 11 (D-6): m-IgG Fc BP-HRP 套装

sc-527626
200 µg Ab; 10 µg BP
$354.00

Gα 11 (D-6): m-IgG1 BP-HRP 套装

sc-532999
200 µg Ab; 20 µg BP
$354.00

Gα 11 抗体 (D-6) AC

sc-390382 AC
500 µg/ml, 25% agarose
$416.00

Gα 11 抗体 (D-6) HRP

sc-390382 HRP
200 µg/ml
$316.00

Gα 11 抗体 (D-6) FITC

sc-390382 FITC
200 µg/ml
$330.00

Gα 11 抗体 (D-6) PE

sc-390382 PE
200 µg/ml
$343.00

Gα 11 抗体 (D-6) Alexa Fluor® 488

sc-390382 AF488
200 µg/ml
$357.00

Gα 11 抗体 (D-6) Alexa Fluor® 546

sc-390382 AF546
200 µg/ml
$357.00

Gα 11 抗体 (D-6) Alexa Fluor® 594

sc-390382 AF594
200 µg/ml
$357.00

Gα 11 抗体 (D-6) Alexa Fluor® 647

sc-390382 AF647
200 µg/ml
$357.00

Gα 11 抗体 (D-6) Alexa Fluor® 680

sc-390382 AF680
200 µg/ml
$357.00

Gα 11 抗体 (D-6) Alexa Fluor® 790

sc-390382 AF790
200 µg/ml
$357.00

Gα 11 (D-6) 中和勝肽

sc-390382 P
100 µg/0.5 ml
$68.00

What is the recommended secondary for this mouse monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2017-02-02
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Rated 5 out of 5 by from Excellent antibody! highly specific!The Sc390382 G-alpha 11 antibody is another excellent mouse monoclonal antibody from SantaCruz, It recognizes human (see my blot), mouse and rat proteins. The specificity is so apparent if you look at the attached blot, the G-alpha 11 knock out cells do not give any non-specific cross reacting bands. Further, you can simultaneously use this antibody with anti-porin (Sc-390996) to quantify relative expression at the same time, saves your time.So, highly recommend this antibody.
Date published: 2017-03-01
Rated 5 out of 5 by from good useThe Gα11 antibody is a mouse monoclonal IgG1 provided at 200 μg / ml. It is recommended to detect mice, rats and blocking peptides by WB, IP, IF, IHC, and ELISA, and has a good use value
Date published: 2017-02-09
Rated 5 out of 5 by from Very specific antibodyWe used this antibody and got a nice specific western blot at the correct molecular weight
Date published: 2017-01-04
Rated 3 out of 5 by from Two bands detected in WBTwo bands detected in WB, very clear and good membrane signal in IF. -SCBT Publication Review
Date published: 2016-03-22
Rated 5 out of 5 by from Good specificity in WB in NIH/3T#Good specificity in WB in NIH/3T#, Jurkat, SK-N-SH whole cell lysates, mouse brain and human hippocampus tissue extracts. -SCBT QC
Date published: 2015-01-16
Rated 5 out of 5 by from Good IHC antibodyGood IHC antibody, strong membrane and cytoplasmic staining of formalin-fixed human gall bladder tissue. -SCBT QC
Date published: 2013-08-16
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G α 11 抗体 (D-6) is rated 4.7 out of 5 by 6.
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