Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
G<sub>&alpha; q/11/14 Antibody (</sub>G-7) - Western Blotting - Image 287901
Gα q/11/14 (G-7): sc-365906. Gα q/11/14 在 NIH/3T3 (A) 和 SK-N-SH (B) 全细胞裂解液以及小鼠大脑 (C) 和人类海马 (D) 组织提取物中的表达的 Western 印迹分析.
Gα q/11/14 (G-7): sc-365906. Gα q/11/14 在 NIH/3T3 (A) 和 SK-N-SH (B) 全细胞裂解液以及小鼠大脑 (C) 和人类海马 (D) 组织提取物中的表达的 Western 印迹分析.

Gα q/11/14 抗体 (G-7): sc-365906

4.1(8)
寫評論提問

说明书
  • G alpha q/11/14 抗体 G-7 是小鼠单克隆 IgG1 κ，Gα q/11/14 抗体, 在24篇文献中引用，规格为200 µg/ml
  • 与小鼠和human的Gα 11的C-terminus对应的60-359氨基酸序列
  • G alpha q/11/14 抗体 (G-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Gα q, Gα 11 and Gα 14
  • 抗G alpha q/11/14抗体（G-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Gα q/11/14 (G-7): sc-365906的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Gα q/11/14 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Gα q/11/14 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Heterotrimeric G Protein 抗体包括 G α 11, G α 12, G α 13, G α i1, G α i2, G α i3, G α o, G α olf, G α q 和 G α q11

Gα q/11/14抗体（G-7）是一种IgG1 κ小鼠单克隆Gα q/11/14抗体（也称为三聚体G蛋白GNAQ抗体、G蛋白亚基α q抗体、GNA11抗体、G蛋白亚基α 11抗体、GNA14抗体、G蛋白亚基α 14抗体或异源三聚体G蛋白抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组化（IHC，P）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人类来源的Gα q/11/14蛋白。Gα q/11/14抗体（G-7）既可以非偶联的抗Gα q/11/14抗体形式提供，也可以多种偶联形式的抗Gα q/11/14抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。异源三聚体G蛋白的功能是将信息从细胞表面受体传递到细胞内效应器。非常广泛的受体中的每一个都特异性地检测细胞外刺激（光子、信息素、气味剂、激素或神经递质），而效应器（例如，腺苷酸环化酶）则产生一种或多种细胞内信使，数量较少。在哺乳动物中，G蛋白α、β和γ多肽分别由至少16、4和7个基因编码。对G蛋白的兴趣主要集中在它们的α亚基上，因为这些蛋白质结合并水解GTP，并且最明显地调节了研究最深入的效应器的活性。已经鉴定出四种不同的Gα亚基类别，包括Gs、Gi、Gq和Gα 12/13。Gq类包括Gα 15、Gα 14、Gα 11和Gα q，其中Gα 11和Gα q在脑和肺中含量丰富，在各种组织中的含量较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gα q/11/14 抗体 (G-7) 参考文献:

  1. 阿片受体信号传导机制。  |  Connor, M. and Christie, MD. 1999. Clin Exp Pharmacol Physiol. 26: 493-9. PMID: 10405772
  2. G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
  3. 异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
  4. Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
  5. 信号转导中 G 蛋白的多样性。  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
  6. G 蛋白的多样性：脊椎动物和无脊椎动物中都有一类不同的 alpha 亚基。  |  Strathmann, M. and Simon, MI. 1990. Proc Natl Acad Sci U S A. 87: 9113-7. PMID: 2123549
  7. 内在光敏感视网膜神经节细胞中通过Gq到腺苷酸环化酶的交叉模式信号传导的不寻常光转换。  |  Chen, L., et al. 2023. Proc Natl Acad Sci U S A. 120: e2216599120. PMID: 36584299
  8. Gαq在调节NLRP3炎症小体激活中的作用。  |  Kong, R., et al. 2024. Inflamm Res. 73: 2249-2261. PMID: 39455437
  9. 杆状病毒-昆虫细胞系统中表达的Gq家族G蛋白GL1 alpha（G14 alpha）、GL2 alpha（G11 alpha）和Gq alpha的表征。  |  Nakamura, F., et al. 1995. J Biol Chem. 270: 6246-53. PMID: 7890762
  10. 与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
  11. G蛋白α亚基的N端区域对爪蟾卵母细胞中磷脂酶C活化的重要性  |  Nakamura, K., et al. 1996. J Biochem. 120: 996-1001. PMID: 8982868
  12. 有证据表明，Gq家族G蛋白在受精时的小鼠卵激活中不起作用。  |  Williams, CJ., et al. 1998. Dev Biol. 198: 116-27. PMID: 9640335

订购信息

产品名称产品编号规格价格数量收藏夹

Gα q/11/14 抗体 (G-7)

sc-365906
200 µg/ml
$316.00

Gα q/11/14 (G-7): m-IgG Fc BP-HRP 套装

sc-529506
200 µg Ab; 10 µg BP
$354.00

Gα q/11/14 (G-7): m-IgGκ BP-HRP 套装

sc-522416
200 µg Ab, 40 µg BP
$354.00

Gα q/11/14 (G-7): m-IgG1 BP-HRP 套装

sc-543548
200 µg Ab; 20 µg BP
$354.00

Gα q/11/14 抗体 (G-7) AC

sc-365906 AC
500 µg/ml, 25% agarose
$416.00

Gα q/11/14 抗体 (G-7) HRP

sc-365906 HRP
200 µg/ml
$316.00

Gα q/11/14 抗体 (G-7) FITC

sc-365906 FITC
200 µg/ml
$330.00

Gα q/11/14 抗体 (G-7) PE

sc-365906 PE
200 µg/ml
$343.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 488

sc-365906 AF488
200 µg/ml
$357.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 546

sc-365906 AF546
200 µg/ml
$357.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 594

sc-365906 AF594
200 µg/ml
$357.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 647

sc-365906 AF647
200 µg/ml
$357.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 680

sc-365906 AF680
200 µg/ml
$357.00

Gα q/11/14 抗体 (G-7) Alexa Fluor® 790

sc-365906 AF790
200 µg/ml
$357.00

I wanted to know which is the dilution rank recommended for this antibody in order to use it in a Western Blot analysis. Thank you so much.

Asked by: LCAb
Thank you for your question. The recommended dilution is 1:100-1:1000. Here is our Western Bolt protocol.https://www.scbt.com/resources/protocols/western-immuno-blotting.If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing scbt@scbt.com or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Blake J
Date published: 2021-07-08

I am working on a non-traditional worm model and was recommended by another colleague that I try this antibody to detect photoreceptor cells in the tail segments of my worm, Lumbriculus variegatus. Is it possible to receive a sample size for testing?

Asked by: vgmmartinez
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-26

Can G&alpha; q/11/14 (G-7): sc-365906 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, G&alpha; q/11/14 (G-7): sc-365906 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_365906, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 13ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Doesn't workThis antibody does not work in a GTP-gamma S binding assay or Western blot (with positive controls working well for the previous sc-393)
Date published: 2018-03-06
Rated 5 out of 5 by from 功能很好我用的小样试用装，做WB的效果很不错，还比较好显色，，，，，建议需要的童鞋放心购买，。。。。。。。。。
Date published: 2017-08-29
Rated 5 out of 5 by from Effect is remarkableGα q/11/14 is the golden antibody. We got obtained specific band is very clear and powerful signal. Very efficient antibody indeed.
Date published: 2017-02-09
Rated 4 out of 5 by from it works good in western blotWe tried this antibody in HDMVECs. It shows a good recognition to GNQA protein.
Date published: 2017-01-23
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Gα q/11/14 expression in NIH/3T3 and SK-N-SH whole cell lysates and mouse brain and human hippocampus tissue extracts.
Date published: 2017-01-17
Rated 4 out of 5 by from Much improvedI received a sample of this antibody as a replacement for the polyclonal E-17 (SC393) that is being discontinued. I compared the two side-by-side, overexpressing Galpha q in knockout cells. For the old polyclonal, the band of interest was barely distinguishable even in an overexpressed system; it was much less intense than dozens of nonspecific bands. For G-7, the band of interest was clear and intense; only one nonspecific band of anywhere close to a comparable intensity appeared, and it was at a much lower molecular weight (<20kDa). I have not attempted to detect endogenous levels of Galpha q with this antibody yet.
Date published: 2016-10-31
Rated 5 out of 5 by from Strong bands at the expected molecular weightStrong bands at the expected molecular weight observed by Western Blot using NIH/3T3, SK-N-SH whole cell lysates and mouse brain, human hippocampus tissue extracts. -SCBT QC
Date published: 2015-01-17
Rated 4 out of 5 by from Strong signal obtained by Western Blot usingStrong signal obtained by Western Blot using HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-07-19
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_365906, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
G α q/11/14 抗体 (G-7) is rated 4.1 out of 5 by 8.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_365906, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 6ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。