Date published: 2025-9-7

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GAD-67 抗体 (F-6): sc-28376

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说明书
  • GAD-67抗体(F-6)是小鼠单克隆IgG3 κ, 在30篇文献中引用,规格为200 µg/ml
  • 免疫human物种的GAD-67的氨基酸1-101
  • 推荐用于 mouse, rat 和 human 来源的GAD-67 WB, IP, IF, IHC(P) 和 ELISA检测
  • 另提供琼脂糖偶联抗体,规格为琼脂糖装,可用于IP实验;HRP 偶联抗体用于WB, IHC(P)和ELISA实验;以及藻红蛋白、FITC、Alexa Fluor® 488Alexa Fluor® 647 偶联抗体,规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取GAD-67 (F-6): sc-28376免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP 是GAD-67 抗体 (F-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与GAD-67 抗体 (F-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

GAD-67抗体(F-6)是一种小鼠单克隆IgG3 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的GAD-67。GAD-67抗体(F-6)有结合和非结合两种形式,可满足各种实验需求。GAD-67,也称为GAD1或SCP,在γ-氨基丁酸(GABA)的合成中起着至关重要的作用,而GABA是大脑中的主要抑制性神经递质。GAD-67对于维持中枢神经系统兴奋和抑制信号的平衡至关重要,而中枢神经系统对于大脑的正常功能和预防神经系统疾病至关重要。GAD-65定位于神经末梢的突触囊泡膜,并在对GABA的需求增加时激活,而GAD-67则分布在整个细胞中,负责GABA合成的基本水平。GAD-67的缺失会导致严重后果,甚至致命,这凸显了GAD-67在神经传递中的重要性。此外,GAD-67还会发生选择性剪接,形成两种同工型:主要同工型GAD-67和次要同工型GAD-25。次要同工型GAD-25不会在大脑中表达,但可在各种内分泌组织中找到。GAD-67抗体(F-6)是研究GABA能信号及其在神经生物学中意义的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAD-67 抗体 (F-6) 参考文献:

  1. 大鼠视网膜发育过程中GAD(65)和GAD(67)的差异表达。  |  Dkhissi, O., et al. 2001. Brain Res. 919: 242-9. PMID: 11701136
  2. 1 型糖尿病患者对 GAD25 的免疫反应性。  |  Chessler, SD., et al. 2002. Autoimmunity. 35: 335-41. PMID: 12515288
  3. 在灵长类动物Cebus apella的网状细胞和间质细胞中,GAD-65和GAD-67与酪氨酸羟化酶和一氧化氮合成酶共存。  |  Andrade da Costa, BL. and Hokoç, JN. 2003. Vis Neurosci. 20: 153-63. PMID: 12916737
  4. 人类胎儿胰腺发育过程中 GAD67 和新型 GAD67 剪接变体的表达:胎儿胰腺中 GAD67 的表达。  |  Korpershoek, E., et al. 2007. Endocr Pathol. 18: 31-6. PMID: 17652798
  5. 狗不同年龄阶段海马 CA1 区谷氨酸脱羧酶 67 免疫反应神经元的比较。  |  Hwang, IK., et al. 2008. Neurosci Lett. 431: 251-5. PMID: 18166269
  6. 环磷酰胺治疗抗GAD抗体阳性的难治性癫痫状态。  |  Kanter, IC., et al. 2008. Epilepsia. 49: 914-20. PMID: 18177354
  7. 感觉缺失经突触改变了成年小鼠主嗅球外部丛状层神经元 GluR1 的表达。  |  Hamilton, KA., et al. 2008. Chem Senses. 33: 201-10. PMID: 18184638
  8. 无佐剂抗原诱导的无害 IFNgamma 通过抑制 IL-17 产物恢复 NOD 小鼠的正常血糖。  |  Jain, R., et al. 2008. J Exp Med. 205: 207-18. PMID: 18195074
  9. 棕榈酰化循环动力学调节 GABA 合成酶 GAD65 在 ER-Golgi 和后高尔基体膜之间的分配。  |  Kanaani, J., et al. 2008. J Cell Sci. 121: 437-49. PMID: 18230651
  10. 大脑中 L-谷氨酸脱羧酶的翻译后调控。  |  Wei, J. and Wu, JY. 2008. Neurochem Res. 33: 1459-65. PMID: 18270816
  11. 在大鼠颈脊髓中,一些γ-肌突元含有γ-氨基丁酸。  |  Ito, T., et al. 2008. Brain Res. 1201: 78-87. PMID: 18294622
  12. 成人海马中GABA神经元中GAD(67)调节与细胞周期和DNA修复的关系:双相情感障碍与精神分裂症。  |  Benes, FM. 2010. Cell Cycle. 9: 625-7. PMID: 20107308

订购信息

产品名称产品编号规格价格数量收藏夹

GAD-67 抗体 (F-6)

sc-28376
200 µg/ml
$316.00

GAD-67 (F-6): m-IgG3 BP-HRP 套装

sc-550546
200 µg Ab; 40 µg BP
$354.00

GAD-67 抗体 (F-6) AC

sc-28376 AC
500 µg/ml, 25% agarose
$416.00

GAD-67 抗体 (F-6) Alexa Fluor® 488

sc-28376 AF488
200 µg/ml
$357.00

GAD-67 抗体 (F-6) Alexa Fluor® 647

sc-28376 AF647
200 µg/ml
$357.00

GAD-67 抗体 (F-6) FITC

sc-28376 FITC
200 µg/ml
$330.00

GAD-67 抗体 (F-6) HRP

sc-28376 HRP
200 µg/ml
$316.00

GAD-67 抗体 (F-6) PE

sc-28376 PE
200 µg/ml
$343.00

How can GAD-67 (F-6): sc-28376 monoclonal antibody used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
Thank you for your question In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Please contact our special orders department for pricing and availability of sc-28376 conjugates.
Answered by: Santa Cruz TS
Date published: 2017-01-30
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Rated 5 out of 5 by from An excellent productAn excellent product; the WB experimental results are perfect. I would recommend it to my classmates
Date published: 2025-08-05
Rated 1 out of 5 by from Poor antibodyTested the antibody in immunofluorescent staining on human iPSC-derived neurons at 1:50 dilution. The antibody gives a rather weak and diffuse staining. Image shows sc-28376 Mouse anti-GAD67 (green), rabbit anti-Synapsin 1 (red), chicken anti-TubB3 (blue), nuclei are counterstained with DAPI (gray).
Date published: 2024-06-13
Rated 5 out of 5 by from This antibody worked wellDiluted 1:500, rat brain tissue in WB experiment, found it works well
Date published: 2017-08-17
Rated 5 out of 5 by from I am very satisfied.This antibody for IF and staining data very strong in mouse brain tissue.
Date published: 2017-05-09
Rated 5 out of 5 by from Produced publishable WB and IHC data on m and rWell established GAD-67 monoclonal with solid data WB and Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse, rat and human samples.
Date published: 2017-01-12
Rated 5 out of 5 by from Robust band seen in WBBands seen in Western blotting using 293T lysate and mouse brain extract
Date published: 2017-01-10
Rated 4 out of 5 by from It' useful.It' useful.But had some background staining that we couldn't reduce with different protocols
Date published: 2016-12-01
Rated 3 out of 5 by from IHC OKIHC OK, but had some background staining that we couldn't reduce with different protocols
Date published: 2016-01-05
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GAD-67 抗体 (F-6) is rated 4.2 out of 5 by 11.
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