Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

G-CSF 抗体 (3D1): sc-53292

4.0(8)
寫評論提問

说明书
  • G-CSF 抗体 3D1 是小鼠单克隆 IgG2a κ,G-CSF抗体, 在13篇文献中引用,规格为200 µg/ml
  • 针对重组human G-CSF
  • G-CSF 抗体 (3D1) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 G-CSF
  • 抗G-CSF抗体(3D1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物的中和研究,sc-53292 L,200 µg/0.1 ml
  • 关于如何获取G-CSF (3D1): sc-53292免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 G-CSF 抗体 (3D1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与G-CSF 抗体 (3D1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

G-CSF抗体(3D1)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测人类来源的G-CSF蛋白。抗粒细胞集落刺激因子抗体(3D1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。粒细胞集落刺激因子(G-CSF)是一种重要的多效细胞因子,在嗜中性粒细胞的分化、增殖和活化中发挥着重要作用,而嗜中性粒细胞对于免疫反应至关重要。G-CSF主要由骨髓基质细胞分泌,作用于造血祖细胞,刺激中性粒细胞的产生,从而增强人体抵抗感染的能力。成熟的G-CSF蛋白由178个氨基酸组成,在30个氨基酸信号肽的切割后,与G-CSF受体(G-CSFR)相互作用,后者是一种跨细胞膜的糖基化多肽。这种相互作用会触发一系列细胞内信号传导通路,包括Ras/MAPK通路,该通路对细胞存活和增殖至关重要。G-CSF已被证明会影响多种激酶和转录因子的激活,如Lyn、Syk和Stat3,从而凸显其在免疫调节和反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

G-CSF 抗体 (3D1) 参考文献:

  1. 抗细胞因子单克隆抗体的开发策略:临床样本中 IL-10 和 IL-5 的免疫测定。  |  Abrams, JS., et al. 1992. Immunol Rev. 127: 5-24. PMID: 1387110
  2. 使用 NIP 标记的抗细胞因子抗体对小鼠和人类细胞因子进行免疫酶测定。  |  Abrams, JS. 2001. Curr Protoc Immunol. Chapter 6: 6.20.1-6.20.15. PMID: 18432811
  3. 中性粒细胞减少症中的 G-CSF 和 GM-CSF。  |  Mehta, HM., et al. 2015. J Immunol. 195: 1341-9. PMID: 26254266
  4. 粒细胞生成的守护者 G-CSF。  |  Mehta, HM. and Corey, SJ. 2021. Semin Immunol. 54: 101515. PMID: 34772606
  5. 人粒细胞集落刺激因子 cDNA 的分子克隆和表达。  |  Nagata, S., et al. Nature. 319: 415-8. PMID: 3484805
  6. 小鼠粒细胞集落刺激因子 cDNA 的分离与鉴定。  |  Tsuchiya, M., et al. 1986. Proc Natl Acad Sci U S A. 83: 7633-7. PMID: 3489940
  7. G-CSF 推动了 APLAID 的自身炎症。  |  Mulazzani, E., et al. 2023. Nat Immunol. 24: 814-826. PMID: 36997670
  8. G-CSF 在急性髓性白血病生物学和治疗中的作用。  |  Visani, G. and Manfroi, S. 1995. Leuk Lymphoma. 18: 423-8. PMID: 8528048
  9. 粒细胞集落刺激因子能迅速激活正常髓系细胞中一种独特的 STAT 类蛋白。  |  Tweardy, DJ., et al. 1995. Blood. 86: 4409-16. PMID: 8541528
  10. 人粒细胞集落刺激因子受体的酪氨酸 764 在 p145/Shc/GRB2 或 p90/GRB2 复合物介导的信号转导中的特异性参与。  |  de Koning, JP., et al. 1996. Blood. 87: 132-40. PMID: 8547634
  11. 粒细胞集落刺激因子(G-CSF)动员的外周血单核细胞中的 CD14+ 细胞诱导骨髓基质分泌白细胞介素-6 和 G-CSF。  |  Mielcarek, M., et al. 1996. Blood. 87: 574-80. PMID: 8555479
  12. 细胞因子剥夺过程中对 32D 细胞凋亡的抑制不足以诱导粒细胞的完全分化。  |  Rodel, JE. and Link, DC. 1996. Blood. 87: 858-64. PMID: 8562953

订购信息

产品名称产品编号规格价格数量收藏夹

G-CSF 抗体 (3D1)

sc-53292
200 µg/ml
$316.00

G-CSF (3D1): m-IgG Fc BP-HRP 套装

sc-525586
200 µg Ab; 10 µg BP
$354.00

G-CSF (3D1): m-IgG2a BP-HRP 套装

sc-546327
200 µg Ab; 10 µg BP
$354.00

G-CSF 抗体 (3D1) L

sc-53292 L
200 µg/0.1 ml
$316.00

G-CSF 抗体 (3D1) AC

sc-53292 AC
500 µg/ml, 25% agarose
$416.00

G-CSF 抗体 (3D1) HRP

sc-53292 HRP
200 µg/ml
$316.00

G-CSF 抗体 (3D1) FITC

sc-53292 FITC
200 µg/ml
$330.00

G-CSF 抗体 (3D1) PE

sc-53292 PE
200 µg/ml
$343.00

G-CSF 抗体 (3D1) Alexa Fluor® 488

sc-53292 AF488
200 µg/ml
$357.00

G-CSF 抗体 (3D1) Alexa Fluor® 546

sc-53292 AF546
200 µg/ml
$357.00

G-CSF 抗体 (3D1) Alexa Fluor® 594

sc-53292 AF594
200 µg/ml
$357.00

G-CSF 抗体 (3D1) Alexa Fluor® 647

sc-53292 AF647
200 µg/ml
$357.00

G-CSF 抗体 (3D1) Alexa Fluor® 680

sc-53292 AF680
200 µg/ml
$357.00

G-CSF 抗体 (3D1) Alexa Fluor® 790

sc-53292 AF790
200 µg/ml
$357.00

I would love to use sc-53292 for my mouse samples. Will it work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-08
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_53292, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Work wellI brought this antibody a month ago and feel lucky to choose this one for WB. I load 30ug mouse liver tissue and use 1:1000 overnight. It can be exposed easily.
Date published: 2017-12-03
Rated 5 out of 5 by from this antibody function well in ElisaThis antibody can be used in ELISA.I used it as capture antibody, works beautifully. It makes great and specific results.BTW, it's stable.
Date published: 2017-02-07
Rated 5 out of 5 by from Clear bands and specific signal.Great G-CSF detection reagent. that works beautifully in immunostaining and gives clear and specific WB bands. It has been published with several times.
Date published: 2017-01-19
Rated 5 out of 5 by from This antibody can also be used in ELISAI developed this ELISA method by myself. This antibody acted as the capture antibody in the sanwich ELISA. The sensitivity meets my requirement.
Date published: 2017-01-11
Rated 3 out of 5 by from Clean band in western blot analysis of humanClean band in western blot analysis of human recombinant G-CSF. -SCBT QC
Date published: 2015-07-07
Rated 3 out of 5 by from Used for western blotting with EscherichiaUsed for western blotting with Escherichia coli strain. -SCBT Publication Review
Date published: 2015-02-15
Rated 3 out of 5 by from Good immunoperoxidase cytoplasmic stainingGood immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2013-11-06
Rated 3 out of 5 by from Acceptable immunoperoxidase cytoplasmic stainingAcceptable immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2013-11-06
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_53292, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
G-CSF 抗体 (3D1) is rated 4.0 out of 5 by 8.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_53292, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT