Date published: 2025-9-7
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frizzled-6 Antibody (C-12) - Western Blotting - Image 359448
frizzled-6 抗体 (C-12): sc-393791. Jurkat (A), CCRF-CEM (B) 和 HT-29 (C) 全细胞裂解液中 frizzled-6 表达的 Western 印迹分析.
frizzled-6 抗体 (C-12): sc-393791. Jurkat (A), CCRF-CEM (B) 和 HT-29 (C) 全细胞裂解液中 frizzled-6 表达的 Western 印迹分析.

frizzled-6 抗体 (C-12): sc-393791

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说明书
  • frizzled-6 抗体 C-12 是小鼠单克隆 IgG2b κ，frizzled-6抗体, 在3篇文献中引用，规格为200 µg/ml
  • 特定于human起源的frizzled-6的N-terminal胞外结构域内氨基酸57-79之间的表位图谱
  • frizzled-6 抗体 (C-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 frizzled-6
  • 抗frizzled-6抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取frizzled-6 (C-12): sc-393791的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是frizzled-6 Antibody (C-12) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与frizzled-6 Antibody (C-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外frizzled抗体，包括frizzled-1, frizzled-2, frizzled-3, frizzled-4, frizzled-5, frizzled-6和frizzled-7

frizzled-6抗体（C-12）是一种IgG2b κ型小鼠单克隆frizzled-6抗体（也被指定为FZD6抗体），能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附试验（ELISA）来检测小鼠、大鼠和人类来源的frizzled-6蛋白。frizzled-6抗体（C-12）既有非偶联的anti-frizzled-6抗体形式，也有多种偶联的anti-frizzled-6抗体形式提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及多种Alexa Fluor®偶联物。Frizzled-6（FZD6，在果蝇中也被称为卷曲同源蛋白6）是一种七次跨膜域受体，与Wnt配体结合，影响宏观毛发生长模式和其他组织极性事件。Frizzled-6蛋白在N端细胞外区域包含一个信号肽和一个富含半胱氨酸的结构域（CRD），并且不包含C端PDZ结构域结合基序。Frizzled-3和Frizzled-6影响神经管闭合以及一部分听觉和前庭感觉细胞上的毛束平面取向。Madin-Darby犬肾（MDCK）细胞能够在体外形成小管，并表达Frizzled-6受体，已知该受体与这种细胞类型中的Wnt-4通过CRD形成复合物。Frizzled-6在各种人体组织中表达为4.4 kb的mRNA，包括成年心脏、大脑、胎盘、肺、肝、骨骼肌、肾、胰腺、胸腺、前列腺、睾丸、卵巢、小肠和结肠。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

frizzled-6 抗体 (C-12) 参考文献:

  1. 人类 Frizzled 6（HFz6）是典型 Wnt 信号级联的负调控因子。  |  Golan, T., et al. 2004. J Biol Chem. 279: 14879-88. PMID: 14747478
  2. Frizzled6控制着小鼠的毛发模式。  |  Guo, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 9277-81. PMID: 15169958
  3. Wnt-4激活典型β-卡替宁介导的Wnt通路并与Frizzled-6 CRD结合：肾上皮细胞中Wnt/β-卡替宁活性的功能性意义。  |  Lyons, JP., et al. 2004. Exp Cell Res. 298: 369-87. PMID: 15265686
  4. 成人肠道中 Wnt 信号成分的表达模式。  |  Gregorieff, A., et al. 2005. Gastroenterology. 129: 626-38. PMID: 16083717
  5. 骨形态发生蛋白-2可调节正常角质形成细胞中Wnt和frizzled的表达，并增强Wnt信号的典型途径。  |  Yang, L., et al. 2006. J Dermatol Sci. 42: 111-9. PMID: 16442268
  6. Frizzled3和Frizzled6在神经管闭合和内耳感觉毛细胞平面极性中的作用  |  Wang, Y., et al. 2006. J Neurosci. 26: 2147-56. PMID: 16495441
  7. Frizzled 6的突变导致孤立的常染色体隐性遗传指甲发育不良。  |  Fröjmark, AS., et al. 2011. Am J Hum Genet. 88: 852-860. PMID: 21665003
  8. 通过FRAP评估Frizzled 6的膜流动性，支持G蛋白偶联，揭示WNT-Frizzled的选择性。  |  Kilander, MB., et al. 2014. Cell Signal. 26: 1943-9. PMID: 24873871
  9. 神经孢子菌中昼夜节律对翻译起始因子eIF2α活性的控制需要eIF2γ依赖性节奏PPP-1磷酸酶的招募。  |  Xie, Z., et al. 2018. Cell Death Differ. 25: 1442-1456. PMID: 29352270
  10. 调控扁平细胞极性蛋白Frizzled-6从内质网中输出的分子机制。  |  Tang, X., et al. 2020. J Biol Chem. 295: 8972-8987. PMID: 32376691
  11. 人类 Frizzled-6 的分子克隆。  |  Tokuhara, M., et al. 1998. Biochem Biophys Res Commun. 243: 622-7. PMID: 9480858
  12. 对无翅信号做出反应的新型果蝇施耐德S2细胞系的鉴定和特征描述  |  Yanagawa, S., et al. 1998. J Biol Chem. 273: 32353-9. PMID: 9822716

订购信息

产品名称产品编号规格价格数量收藏夹

frizzled-6 抗体 (C-12)

sc-393791
200 µg/ml
$316.00

frizzled-6 (C-12): m-IgG2b BP-HRP 套装

sc-548782
200 µg Ab; 10 µg BP
$354.00

frizzled-6 抗体 (C-12) AC

sc-393791 AC
500 µg/ml, 25% agarose
$416.00

frizzled-6 抗体 (C-12) HRP

sc-393791 HRP
200 µg/ml
$316.00

frizzled-6 抗体 (C-12) FITC

sc-393791 FITC
200 µg/ml
$330.00

frizzled-6 抗体 (C-12) PE

sc-393791 PE
200 µg/ml
$343.00

frizzled-6 抗体 (C-12) Alexa Fluor® 488

sc-393791 AF488
200 µg/ml
$357.00

frizzled-6 抗体 (C-12) Alexa Fluor® 546

sc-393791 AF546
200 µg/ml
$357.00

frizzled-6 抗体 (C-12) Alexa Fluor® 594

sc-393791 AF594
200 µg/ml
$357.00

frizzled-6 抗体 (C-12) Alexa Fluor® 647

sc-393791 AF647
200 µg/ml
$357.00

frizzled-6 抗体 (C-12) Alexa Fluor® 680

sc-393791 AF680
200 µg/ml
$357.00

frizzled-6 抗体 (C-12) Alexa Fluor® 790

sc-393791 AF790
200 µg/ml
$357.00

frizzled-6 (C-12) 中和勝肽

sc-393791 P
100 µg/0.5 ml
$68.00

We saw a strong band just below the 50 kD marker in HCT116 whole cell extract, but no clear band above it with 1:500 dilution from your sample antibody by western blot. What concentration did you use to detect Frizzed6 protein in HT29 cells?

Asked by: Cell
Thank you for your question. frizzled-6 (C-12) is recommended for detection of CMAS of mouse, rat, and human origin by western blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:300).
Answered by: Tech Support Europe
Date published: 2021-03-03

What application is the blocking peptide sc-393791 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from It's great!I bought this about a month ago, and it works very well in both month and guinea pig tissue for immunofluorescence staining.
Date published: 2017-11-16
Rated 5 out of 5 by from Nice and strong bandsNice single bands detected at 70kDa size when tested in primary mouse T cells. Much better than frizzled-6 Antibody (D-2): sc-393113.
Date published: 2017-11-10
Rated 5 out of 5 by from Good Western Blot data of frizzledGood Western Blot data of frizzled-6 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from Great Western Blot data of frizzledGreat Western Blot data of frizzled-6 expression in CCRF-CEM and HuT 78 whole cell lysates. -SCBT QC
Date published: 2014-02-07
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frizzled-6 抗体 (C-12) is rated 5.0 out of 5 by 4.
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