Flg抗体 (M5G10) 推荐用于 mouse, rat 和 human 来源的Flg isoforms α and β WB, IP, IF 和 IHC(P)检测

Flg 抗体 (M5G10): sc-57133

Flg抗体 (M5G10) is rated 4.5 out of 5 by 2.
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说明书

    • Flg抗体(M5G10)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • raised against the ectodomain of Flg of human origin
    • 推荐用于 mouse, rat 和 human 来源的Flg isoforms α and β WB, IP, IF 和 IHC(P)检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Flg (M2F12): sc-57132,可查看有标记的 Flg 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
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How much sample do you recommend loading on the gel for Western blot detection using Flg (M5G10): sc-57133?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Sharp bands on human VSMCs the sample antibody gives very sharp bands at ~90 kDa, a little bit smaller than that is predicted, and that showed in the datasheet
Date published: 2017-07-20
Rated 4 out of 5 by from Good specificity in WB in human Flg transfected Good specificity in WB in human Flg transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-08-26
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