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Fis1 抗体 (C-10): sc-376469

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说明书
  • Fis1抗体(C-10)是小鼠单克隆IgG1 κ, 在14篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长Fis1的氨基酸1-152
  • 抗-Fis1 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Fis1
  • 抗Fis1抗体(C-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fis1 (C-10): sc-376469免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Fis1 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Fis1 Antibody (C-10) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Fis1 抗体 (C-10) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 检测小鼠、大鼠和人样本中的 Fis1 蛋白。Fis1 (C-10) 抗体有非结合型和与琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 变体结合型。Fis1 或线粒体裂变 1 蛋白可调节线粒体动力学,特别是线粒体裂变,从而维持健康的细胞功能。Fis1 位于线粒体外膜上,与动态相关蛋白 1(Drp1)一起促进线粒体分裂。Fis1 的 C 端结构域实现线粒体定位,而其 N 端结构域则驱动分裂活动。Fis1 功能障碍导致的线粒体分裂紊乱可诱发神经退行性疾病和癌症,这凸显了 Fis1 在细胞健康和疾病中的重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Fis1 抗体 (C-10)

sc-376469
200 µg/ml
$316.00

Fis1 (C-10): m-IgGκ BP-HRP 套装

sc-522961
200 µg Ab, 40 µg BP
$354.00

Fis1 抗体 (C-10) AC

sc-376469 AC
500 µg/ml, 25% agarose
$416.00

Fis1 抗体 (C-10) HRP

sc-376469 HRP
200 µg/ml
$316.00

Fis1 抗体 (C-10) FITC

sc-376469 FITC
200 µg/ml
$330.00

Fis1 抗体 (C-10) PE

sc-376469 PE
200 µg/ml
$343.00

Fis1 抗体 (C-10) Alexa Fluor® 488

sc-376469 AF488
200 µg/ml
$357.00

Fis1 抗体 (C-10) Alexa Fluor® 546

sc-376469 AF546
200 µg/ml
$357.00

Fis1 抗体 (C-10) Alexa Fluor® 594

sc-376469 AF594
200 µg/ml
$357.00

Fis1 抗体 (C-10) Alexa Fluor® 647

sc-376469 AF647
200 µg/ml
$357.00

Fis1 抗体 (C-10) Alexa Fluor® 680

sc-376469 AF680
200 µg/ml
$357.00

Fis1 抗体 (C-10) Alexa Fluor® 790

sc-376469 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Fis1 (C-10): sc-376469 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from tolerableworks but isn't great ................................
Date published: 2022-05-18
Rated 2 out of 5 by from okweak band detected at 18KDa, high background signal
Date published: 2020-02-06
Rated 5 out of 5 by from Good western blottingI've tested free sample sc-376469 in western blotting of total extract of proximal tubular epitelial cells. 1:1000 o/n 4°C works wery well.
Date published: 2019-06-17
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmicProduced exceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Satisfactory Western Blot data of Fis1 expressionSatisfactory Western Blot data of Fis1 expression in A-431 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-03-03
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Fis1 抗体 (C-10) is rated 4.0 out of 5 by 5.
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