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Fibronectin Antibody (IST-9) - Western Blotting - Image 380652
纤连蛋白抗体 (IST-9): sc-59826. Caki-1 (A), XP12RO (B) 和 A-10 (C) 全细胞裂解液中纤维连接蛋白表达的 Western 印迹分析.
纤连蛋白抗体 (IST-9): sc-59826. Caki-1 (A), XP12RO (B) 和 A-10 (C) 全细胞裂解液中纤维连接蛋白表达的 Western 印迹分析.

Fibronectin 抗体 (IST-9): sc-59826

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寫評論提問
说明书
  • Fibronectin抗体(IST-9)是小鼠单克隆IgG1 κ, 在81篇文献中引用，规格为200 µg/ml
  • 针对cellular Fibronectin human来源的ED-A结构域中的区域
  • 抗-Fibronectin 抗体 (IST-9) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human, porcine 和canine 来源的 cellular Fibronectin
  • 抗Fibronectin抗体（IST-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Fibronectin Antibody (IST-9) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Fibronectin Antibody (IST-9) 打包提供（请参阅下面的订购信息）。
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関連項目

纤维蛋白原抗体（IST-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人、猪和犬样本中的纤维蛋白原。抗纤维蛋白原抗体（IST-9）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。纤维连接蛋白是一种重要的细胞外基质糖蛋白，在细胞粘附、运动和伤口愈合中发挥着重要作用，因此纤维连接蛋白对于组织修复和再生至关重要。这种高分子量异二聚体蛋白主要位于细胞表面、细胞外液和血浆中，纤维连接蛋白可促进细胞与细胞外基质之间的相互作用。纤维粘连蛋白的替代性剪接和糖基化可产生多种同种型，其中一些与特定组织或病理状况（如恶性肿瘤）有关。糖基化纤维粘连蛋白和纤维粘连蛋白剪接变体的存在与杜普伊特伦氏病中肌成纤维细胞表型的形成有关，这凸显了在疾病背景下了解翻译后修饰的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibronectin 抗体 (IST-9) 参考文献:

  1. 对纤维粘连蛋白 III 型结构域介导的信号传导的结构洞察。  |  Bencharit, S., et al. 2007. J Mol Biol. 367: 303-9. PMID: 17261313
  2. 纤连蛋白的肝素 III 结合结构域（III4-5 重复序列）与纤连蛋白结合并抑制纤连蛋白基质组装。  |  Maqueda, A., et al. 2007. Matrix Biol. 26: 642-51. PMID: 17611093
  3. 纤连蛋白在纳米级混合地形上的分布。  |  Pérez-Garnes, M., et al. 2011. Int J Artif Organs. 34: 54-63. PMID: 21298616
  4. 整合素α3β1与纤连蛋白的结合依赖于第九个III型重复序列。  |  Brown, AC., et al. 2015. J Biol Chem. 290: 25534-47. PMID: 26318455
  5. 人类黑色素瘤抑制蛋白与纤维粘连蛋白的 FN12-14 Hep II 结构域结合。  |  Yip, KT., et al. 2017. Biointerphases. 12: 02D415. PMID: 28565914
  6. 与正常细胞相比，转化后的人体细胞会释放出不同的纤维粘连蛋白变体。  |  Castellani, P., et al. 1986. J Cell Biol. 103: 1671-7. PMID: 3023390
  7. 胎牛血清纤连蛋白和内源性大鼠细胞纤连蛋白在细胞外基质中的分布。  |  Hayman, EG. and Ruoslahti, E. 1979. J Cell Biol. 83: 255-9. PMID: 389940
  8. 针对胎盘上纤维粘连蛋白的泛素衍生人工结合蛋白通过β链滑动揭示支架的可塑性  |  Katzschmann, A., et al. 2024. Commun Biol. 7: 907. PMID: 39068227
  9. 人类纤连蛋白代谢。  |  Pussell, BA., et al. 1985. J Clin Invest. 76: 143-8. PMID: 4019773
  10. 细胞纤连蛋白对培养软骨母细胞作用的特异性。  |  West, CM., et al. 1984. Differentiation. 27: 67-73. PMID: 6468805

订购信息

产品名称产品编号规格价格数量收藏夹

Fibronectin 抗体 (IST-9)

sc-59826
200 µg/ml
$322.00

Fibronectin (IST-9): m-IgG Fc BP-HRP 套装

sc-526943
200 µg Ab; 10 µg BP
$361.00

Fibronectin (IST-9): m-IgG1 BP-HRP 套装

sc-532316
200 µg Ab; 20 µg BP
$361.00

Fibronectin 抗体 (IST-9) AC

sc-59826 AC
500 µg/ml, 25% agarose
$424.00

Fibronectin 抗体 (IST-9) HRP

sc-59826 HRP
200 µg/ml
$322.00

Fibronectin 抗体 (IST-9) FITC

sc-59826 FITC
200 µg/ml
$336.00

Fibronectin 抗体 (IST-9) PE

sc-59826 PE
200 µg/ml
$349.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 488

sc-59826 AF488
200 µg/ml
$364.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 546

sc-59826 AF546
200 µg/ml
$364.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 594

sc-59826 AF594
200 µg/ml
$364.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 647

sc-59826 AF647
200 µg/ml
$364.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 680

sc-59826 AF680
200 µg/ml
$364.00

Fibronectin 抗体 (IST-9) Alexa Fluor® 790

sc-59826 AF790
200 µg/ml
$364.00

For IST-9 there is a picture of kidney tissue stained for IHC using IST-9 antibody. Can you please give me the reference for the image as i am looking for the IHC protocol used to produce this image i.e. processing and antigen optimisation process used.

Asked by: Pvirdee
Thanks for your question. The image is the results of in-house validation by Immunoperoxidase staining of formalin fixed, paraffin-embedded. You can find our protocol here https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining . Please contact technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2022-11-09

Hi Can I get a free sample of this antibody. (Fibronectin Antibody (IST-9): sc-59826) I wanted to see if it works with human sample for western blot. thank you Sindhu Saraswathy PhD Scientist Doheny Eye Institute

Asked by: Sindhu Saraswathy
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Does this antibody work for IF of formalin-fixed paraffin-embedded tissue?

Asked by: PhD Student
Thank you for your question. Yes, this antibody is recommended for IF and IHC(P) and will work perfectly fine for IF on formalin fixed tissues. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-10

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. For IHC, we recommend using a directly conjugated mouse monoclonal primary antibody. We recommend using SC-8422 for Fibronectin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 2 out of 5 by Katie from no bandI used this antibody at 1:200 - 1:1000 as 1st antibody with 2nd mouse antibody at 1:1000 - 1:5000. Both 1st and 2nd antibody I dilute with 5% skim milk but still doesn't work. even on semi-dry or wet transfer also showed no band. Any kind suggestion please?
Date published: 2024-03-25
Rated 4 out of 5 by Agnes2004 from Great productIt is good antibodies in WB. It works in dilution 1:1000
Date published: 2018-07-14
Rated 2 out of 5 by MasaT from Strong background in IF on rat frozen liverRat liver frozen sections were subjected to immunofluorescence using Fibronectin (IST-9) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Some signals could be observed in portal tracts, but background was strong.
Date published: 2018-03-14
Rated 4 out of 5 by manash from Works wellI received free samples and tried on western blotting. Tha bands are thick with no secondary bands. Need to dilute antibody concentrations to get good data.
Date published: 2018-02-12
Rated 5 out of 5 by Nguyen from So nice!I received this Ab in mid-November 2017 and was very pleased to see it worked nicely! Will definitely purchase it...
Date published: 2018-01-13
Rated 3 out of 5 by SBos from Staining of distal colon for fibronectin EDAI've had the chance to test the antibody for the staining of fibronectin EDA in the distal colon of mice that were developing fibrosis in the colon. Unfortunately, we did not see staining of the paraffin embedded slides, which also means that there is no background staining and the antibody is very specific. We are currently still trying to change some aspects of the staining protocol to check if we can get better staining.
Date published: 2017-12-04
Rated 5 out of 5 by mingL from clear bandThis is a great antibody, the band was clear and significant.
Date published: 2017-05-13
Rated 5 out of 5 by FengXing from The band is clearlyWe detected positive signal in WB using mouse lung, strong signal;
Date published: 2017-04-22
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Fibronectin 抗体 (IST-9) is rated 4.2 out of 5 by 13.
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