Date published: 2025-9-8
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Estrogen Receptor alpha Antibody (H226) - Western Blotting - Image 139543
雌激素受体 α (H226): sc-53493. 未经处理 (A) 和经 Radicicol (sc-200620) 处理 (B) 的 MCF7 全细胞裂解液中雌激素受体 α 表达的 Western 印迹分析. 注意 B 道中雌激素受体 α 表达的下调.
雌激素受体 α (H226): sc-53493. 未经处理 (A) 和经 Radicicol (sc-200620) 处理 (B) 的 MCF7 全细胞裂解液中雌激素受体 α 表达的 Western 印迹分析. 注意 B 道中雌激素受体 α 表达的下调.

Estrogen Receptor alpha 抗体 (H226): sc-53493

4.6(7)
寫評論提問

说明书
  • Estrogen Receptor alpha 抗体 H226 是大鼠单克隆 IgG1, 在9篇文献中引用，提供 200 µg/ml
  • 免疫human物种的Estrogen Receptor alpha
  • 抗-Estrogen Receptor alpha 抗体 (H226) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Estrogen Receptor alpha
  • 抗Estrogen Receptor alpha抗体（H226）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Estrogen Receptor alpha (H226): sc-53493的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Estrogen Receptor alpha 抗体 (H226)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Estrogen Receptor 抗体包括 Estrogen Receptor alpha, ER β, ERR α, ERR β 和 ERR γ

雌激素受体α抗体（H226）是针对人类雌激素受体α（ERα）制备的大鼠单克隆 IgG1 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类ERα。ERα蛋白是一种配体激活的转录因子，属于类固醇激素受体超家族，在调节生殖组织基因表达方面起着关键作用。ERα与特定蛋白质相互作用，从而调节活性和功能。例如，ERα 与类固醇受体辅激活因子-1（SRC-1）和核受体辅激活因子 3（NCOA3）等辅激活因子结合，从而增强转录活性。此外，ERα 还与核受体核心压制因子（如核受体核心压制因子（NCoR）和视黄醇和甲状腺受体沉默介导因子（SMRT））相互作用，从而抑制转录活性。这些相互作用至关重要，因为它们能微调 ERα 介导的基因表达，影响细胞的增殖、分化和凋亡等过程。这些蛋白质相互作用的失调会导致细胞功能异常，并与乳腺癌等激素依赖性癌症的发生有关。雌激素受体α（H226）单克隆抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种 Alexa Fluor® 共轭物，为研究 ERα 的相互作用、功能和在疾病中的作用提供了广泛的研究应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Estrogen Receptor alpha 抗体 (H226)

sc-53493
200 µg/ml
$316.00

Estrogen Receptor alpha 抗体 (H226) AC

sc-53493 AC
500 µg/ml, 25% agarose
$416.00

Estrogen Receptor alpha 抗体 (H226) HRP

sc-53493 HRP
200 µg/ml
$316.00

Estrogen Receptor alpha 抗体 (H226) FITC

sc-53493 FITC
200 µg/ml
$330.00

Estrogen Receptor alpha 抗体 (H226) PE

sc-53493 PE
200 µg/ml
$343.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 488

sc-53493 AF488
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 546

sc-53493 AF546
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 594

sc-53493 AF594
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 647

sc-53493 AF647
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 680

sc-53493 AF680
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (H226) Alexa Fluor® 790

sc-53493 AF790
200 µg/ml
$357.00

Is it possible to get a sample of the ERα Antibody (H226): sc-53493? We would like to test this antibody in mouse brain sections.

Asked by: Takako
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I would like to receive a sample of the ER H226 antibody to test as a replacement for the discontinued HC20. Thank you.

Asked by: warde005
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2021-10-01

Does your company have any test small volume of ER-alpha(H226):sc-53493, ER-alpha (H222):sc-53492, and ER-alpha (2Q418):sc-71064 Abs? If it works in our samples after test, we are going to order more Abs?

Asked by: shlyang
Thank you for the question. We currently offer free Sample sizes for 5 different monoclonal ER alpha antibodies. For clone (2Q418) we currently do not offer a Sample size. Please contact us by email to customercare @ scbt.com and provide your full shipping address and we will be happy to send you available ER alpha antibody Sample sizes.
Answered by: Technical Support Europe
Date published: 2017-12-29

I am using ERα (H226): sc-53493 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2022-03-30
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Rated 4 out of 5 by from GoodI bought this antibody to perform IHC on liver sample. It was very good ab (see the pictures).
Date published: 2024-05-23
Rated 5 out of 5 by from GreatGot good result. Used it to detect ER alpha in Brest cancer tumor section by immunohistochemistry.
Date published: 2018-08-13
Rated 5 out of 5 by from Produced publishable WB and IHC(P) dataThis ER alpha monocloncal antibody has citations in the literature for the use of WB in rat uterus and femurs and human neuroblastoma cells and IHC(P) data in rat smaples.
Date published: 2017-01-27
Rated 5 out of 5 by from Robust nuclear staining in paraffin embeddedRobust nuclear staining in paraffin embedded human breast. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from Good Western blot analysis of ER expressionGood Western blot analysis of ER expression in untreated and Radicicol treated MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong ER band detected in MCF7 nuclear extract, and T-47D and ZR-75-1 whole cell lysates under reducing conditions -SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Good specificity and signal strength withGood specificity and signal strength with some background in MCF7 nuclear extract and T-47D whole cell lysate under non-reducing conditions. -SCBT QC
Date published: 2013-12-23
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Estrogen Receptor alpha 抗体 (H226) is rated 4.6 out of 5 by 7.
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