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Estrogen Receptor alpha 抗体 (10H12B10): sc-130072

2.0(1)
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说明书
  • Estrogen Receptor alpha抗体(10H12B10)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 与重组蛋白结合,该重组蛋白与Estrogen Receptor alpha的301-595的氨基酸对应,来源于human
  • 推荐用于 human 来源的Estrogen Receptor alpha WB, IP 和 ELISA检测
  • 1 BP-HRP">m-IgG1 BP-HRP是Estrogen Receptor alpha Antibody (10H12B10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Estrogen Receptor alpha Antibody (10H12B10) 打包提供(请参阅下面的订购信息)。

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関連項目

雌激素受体(ER)是类固醇/甲状腺激素受体超家族的成员,属于配体激活的转录因子。雌激素受体,包括ERα和ERβ,含有DNA结合域和配体结合域,在调节生殖组织的正常功能中起着至关重要的作用。它们位于细胞核中,尽管一些雌激素受体与细胞表面膜相关,并可通过细胞暴露于雌激素而迅速激活。已显示ERα和ERβ可由各种配体以不同的方式激活。受体-配体相互作用触发一系列事件,包括与热休克蛋白的解离、受体二聚化、磷酸化以及与靶基因中特定调节元件的激素激活受体的关联。有证据表明,尽管ERα和ERβ具有许多功能特性,但它们可能受不同机制的调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Estrogen Receptor alpha 抗体 (10H12B10) 参考文献:

  1. 雌性大鼠下丘脑中雌激素受体 mRNA 和孕酮受体 mRNA 在衰老过程中的雌激素调控变化:原位杂交研究。  |  Funabashi, T., et al. 2000. Neurosci Res. 38: 85-92. PMID: 10997581
  2. 雌激素受体α在小鼠睾丸和输出小管中具有功能作用。  |  Lee, KH., et al. 2000. Biol Reprod. 63: 1873-80. PMID: 11090460
  3. 在培养的人子宫内膜细胞中调节雌激素受体α和孕激素受体(同型A和B)的表达。  |  Prange-Kiel, J., et al. 2001. Exp Clin Endocrinol Diabetes. 109: 231-7. PMID: 11453036
  4. 模拟二乙基雌酚(DES)对雌激素受体α和雌激素相关受体γ的不同生物学活性。  |  Nam, K., et al. 2003. Biopolymers. 68: 130-8. PMID: 12579585
  5. 小鼠脑中雌激素受体β的免疫定位:与雌激素受体α的比较。  |  Mitra, SW., et al. 2003. Endocrinology. 144: 2055-67. PMID: 12697714
  6. 雌二醇对雌激素受体α基因表达和活性的影响可通过ErbB2/PI 3-K/Akt途径调节。  |  Stoica, GE., et al. 2003. Oncogene. 22: 7998-8011. PMID: 12970748
  7. p130Cas与雌激素受体α相互作用,调节乳腺癌细胞中非基因组雌激素信号。  |  Cabodi, S., et al. 2004. J Cell Sci. 117: 1603-11. PMID: 15020686
  8. ICI182,780对雌激素受体α的非经典调节。  |  Wang, MM., et al. 2004. J Steroid Biochem Mol Biol. 92: 51-62. PMID: 15544930
  9. 雌激素受体-{alpha}的变体hER-{alpha}36:雌激素和抗雌激素依赖性膜启动的促有丝分裂信号转导。  |  Wang, Z., et al. 2006. Proc Natl Acad Sci U S A. 103: 9063-8. PMID: 16754886
  10. 雌激素受体α或β的缺失消除了正常和发炎小鼠在机械疼痛阈值方面的性别差异。  |  Li, L., et al. 2009. Pain. 143: 37-40. PMID: 19285805

订购信息

产品名称产品编号规格价格数量收藏夹

Estrogen Receptor alpha 抗体 (10H12B10)

sc-130072
200 µg/ml
$316.00

Estrogen Receptor alpha (10H12B10): m-IgG1 BP-HRP 套装

sc-542634
200 µg Ab; 20 µg BP
$354.00

ER alpha (10H12B10) antibodies is it available for use in immunohistochemistry or can it be produced

Asked by: Shanthala
Thank you for your question. We do not currently recommend this specific clone for immunohistochemistry (IF or IHC(P)). There are several different monoclonal antibodies against ERalpha that we have tested and do recommend for this application. Please contact our Technical Service department for a product recommendation.
Answered by: Technical Support
Date published: 2019-02-02

How much sample do you recommend loading on the gel for Western blot detection using ERα (10H12B10): sc-130072?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_8, d_27, h_4CST
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Rated 2 out of 5 by from Able to detect ER alpha in recombinant samplesAble to detect ER alpha in recombinant samples, some smearing. -SCBT QC
Date published: 2013-11-03
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Estrogen Receptor alpha 抗体 (10H12B10) is rated 2.0 out of 5 by 1.
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