ERp57抗体 (MaP.ERp57) 推荐用于 mouse, rat 和 human 来源的ERp57 WB, IP, IF 和 IHC(P)检测

ERp57 抗体 (MaP.ERp57): sc-23886

ERp57抗体 (MaP.ERp57) is rated 5.0 out of 5 by 13.
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说明书

    • 抗-ERp57 抗体 MaP.ERp57 是小鼠单克隆 IgG1 κ,ERp57抗体, 在21篇文献中引用,规格为200 µg/ml
    • raised against recombinant human ERp57
    • 抗-ERp57 抗体 (MaP.ERp57) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 ERp57; 也和以下物种反应,包括: canine
    • 抗-ERp57 抗体 (MaP.ERp57) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取ERp57 (MaP.ERp57): sc-23886免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 ERp57 Antibody (MaP.ERp57) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供ERp57 Antibody (MaP.ERp57)。 (见下方订购信息).
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I am using ERp57 (MaP.ERp57): sc-23886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good staining works well analysis ERp57 in fixed mouse rain by IF. Antibody gives high signal and low background, Good staining.
Date published: 2017-08-26
Rated 5 out of 5 by from IHC analysis The staining shows reliable on paraffin human skin tissue. Best results was performed for 1:100 antibody dilution , with the IHC staining kit from Dako.
Date published: 2017-07-29
Rated 5 out of 5 by from Very dark band produced in western blot We used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from nice quality! Distinct band is detected using this product! Nice quality! Got satisfactory results!
Date published: 2016-11-21
Rated 5 out of 5 by from Robust positive signal detected in WB in HeLa Robust positive signal detected in WB in HeLa , Daudi , MDCK and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Published data for WB and IF in human CD34+ cells Published data for WB and IF in human CD34+ cells. -SCBT Publication Review
Date published: 2015-05-24
Rated 5 out of 5 by from Published data for WB in human epithelial Published data for WB in human epithelial ovarian cancer cell line. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Band of interest observed in WB in both non Band of interest observed in WB in both non-transfected 293T and human ERp57 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-10
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