Date published: 2025-9-6
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ErbB4/HER4 Antibody (C-7) - Western Blotting - Image 376090
ErbB-4 抗体 (C-7): sc-8050. MCF7 (A) 和 IMR-32 (B) 全细胞裂解液中 ErbB-4 表达的 Western 印迹分析.
ErbB-4 抗体 (C-7): sc-8050. MCF7 (A) 和 IMR-32 (B) 全细胞裂解液中 ErbB-4 表达的 Western 印迹分析.

ErbB4/HER4 抗体 (C-7): sc-8050

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寫評論提問

说明书
  • ErbB4/HER4 抗体 C-7 是小鼠单克隆 IgG2a κ，ErbB4/HER4抗体, 在52篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ErbB-4的C-terminus的氨基酸1291-1308之间
  • ErbB4/HER4 抗体 (C-7) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA ，检测 mouse, rat, human 和 来源的ErbB-4; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗ErbB4/HER4抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ErbB4/HER4 (C-7): sc-8050的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 ErbB4/HER4 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ErbB4/HER4 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ErbB 抗体 包括 ErbB-4

ErbB-4抗体（C-7）是一种针对人类ErbB-4的小鼠单克隆IgG2a kappa轻链抗体，ErbB-4是一种复杂的信号蛋白，属于表皮生长因子受体家族。ErbB-4小鼠单克隆抗体（C-7）可识别ErbB-4免疫原序列中的表位，并表现出广泛的物种交叉反应性，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的ErbB-4。抗ErbB-4单克隆抗体（C-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物，可满足各种实验需求。ErbB-4通过蛋白水解作用经历独特的调节机制：当ErbB-4的配体heregulin结合时，ErbB-4经历一个裂解过程，释放出胞外结构域，启动关键的下游信号级联反应。这种复杂的控制机制对于正常的细胞功能至关重要，在癌症生物学中具有特殊意义，特别是在乳腺癌中，ErbB-4的表达水平显着升高。ErbB-4在细胞膜上保持精确的定位，在那里ErbB-4可以与其他受体家族成员形成同源二聚体或异源二聚体。这些二聚体复合物形成复杂的信号网络，维持细胞内环境平衡，并协调对外部生长信号的反应。通过这些不同的细胞功能，ErbB-4（C-7）抗体成为研究正常细胞信号传导和癌症发展机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ErbB4/HER4 抗体 (C-7) 参考文献:

  1. Notch-1信号调节巨噬细胞极化以及免疫防御炎症性疾病中的鸟分枝杆菌感染。  |  Carraway, KL., et al. 1997. J Mammary Gland Biol Neoplasia. 2: 187-98. PMID: 10882304
  2. 依赖于Heregulin的ErbB-4贩运和裂解。  |  Zhou, W. and Carpenter, G. 2000. J Biol Chem. 275: 34737-43. PMID: 10944525
  3. HER2 的基础生物学。  |  Rubin, I. and Yarden, Y. 2001. Ann Oncol. 12 Suppl 1: S3-8. PMID: 11521719
  4. erbB-1和erbB-4受体协同作用，促进女性性发育和成熟的生殖功能。  |  Prevot, V., et al. 2005. Endocrinology. 146: 1465-72. PMID: 15591145
  5. ErbB-4和TNF-α转化酶定位到膜微区。  |  Thiel, KW. and Carpenter, G. 2006. Biochem Biophys Res Commun. 350: 629-33. PMID: 17027649
  6. 靶向辐照头颈癌中的 ErbB-1 和 ErbB-4：体外和体内研究的结果。  |  Barnea, I., et al. 2013. Head Neck. 35: 399-407. PMID: 22367849
  7. 人类 HER4/erbB-4 基因在 2 号染色体上的定位。  |  Zimonjic, DB., et al. 1995. Oncogene. 10: 1235-7. PMID: 7700649
  8. 配体特异性激活表皮生长因子受体家族的第四个成员 HER4/p180erbB4。  |  Plowman, GD., et al. 1993. Proc Natl Acad Sci U S A. 90: 1746-50. PMID: 8383326
  9. 蛋白激酶 C 激活选择性地裂解 heregulin 受体 ErbB-4。  |  Vecchi, M., et al. 1996. J Biol Chem. 271: 18989-95. PMID: 8702564
  10. 酪氨酸磷酸化和蛋白水解。Pervanadate诱导金属蛋白酶依赖性地裂解ErbB-4受体和amphiregulin。  |  Vecchi, M., et al. 1998. J Biol Chem. 273: 20589-95. PMID: 9685416
  11. c-erbB-4/HER4 蛋白和 mRNA 在正常人类胎儿和成人组织以及九种实体瘤类型调查中的表达。  |  Srinivasan, R., et al. 1998. J Pathol. 185: 236-45. PMID: 9771476
  12. ErbB-4 mRNA表达在非转移性胰腺癌中降低。  |  Graber, HU., et al. 1999. Int J Cancer. 84: 24-7. PMID: 9988227

订购信息

产品名称产品编号规格价格数量收藏夹

ErbB4/HER4 抗体 (C-7)

sc-8050
200 µg/ml
$316.00

ErbB4/HER4 (C-7): m-IgG Fc BP-HRP 套装

sc-528215
200 µg Ab; 10 µg BP
$354.00

ErbB4/HER4 (C-7): m-IgGκ BP-HRP 套装

sc-520538
200 µg Ab, 40 µg BP
$354.00

ErbB4/HER4 抗体 (C-7) AC

sc-8050 AC
500 µg/ml, 25% agarose
$416.00

ErbB4/HER4 抗体 (C-7) HRP

sc-8050 HRP
200 µg/ml
$316.00

ErbB4/HER4 抗体 (C-7) FITC

sc-8050 FITC
200 µg/ml
$330.00

ErbB4/HER4 抗体 (C-7) PE

sc-8050 PE
200 µg/ml
$343.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 488

sc-8050 AF488
200 µg/ml
$357.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 546

sc-8050 AF546
200 µg/ml
$357.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 594

sc-8050 AF594
200 µg/ml
$357.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 647

sc-8050 AF647
200 µg/ml
$357.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 680

sc-8050 AF680
200 µg/ml
$357.00

ErbB4/HER4 抗体 (C-7) Alexa Fluor® 790

sc-8050 AF790
200 µg/ml
$357.00

ErbB4/HER4 (C-7) 中和勝肽

sc-8050 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-8050 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 5
Date published: 2017-01-22
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Rated 1 out of 5 by from Did not work for immunofluorescence (pig)Immunohistochemistry: the antibody was tested on mammary gland and kidney from 6 months old gilts. Antigen retrieval was performed in citrate buffer, 30'. Primary antibody: 1:50 dilution, 1h, RT. Secondary antibody: Alexa Fluor 488 (1:500), 1h, RT. Results: negative; nonspecific signal was observed both in epithelial and connective fractions.
Date published: 2021-02-01
Rated 5 out of 5 by from Works very well for flow cytommetryWe purchased this item for our flow cytommetry experiments. We are very pleased with the results.
Date published: 2018-04-23
Rated 5 out of 5 by from The baseband is perfectErbB-4 Antibody is the best I have ever used.We got obtained specific band is very clear and signal is powerful. The effect is amazing.
Date published: 2017-10-17
Rated 5 out of 5 by from Western blotting in mouseWestern Blot, 1:500 dilution, it works well, and the detection efficiency of this antibody is very high, I love this antibody.
Date published: 2017-05-22
Rated 5 out of 5 by from Very popular choice!The (C-7) clone for ErbB-4 is very well characterized with over 30 known citations in the literature using the antibody for a variety of research applications.
Date published: 2016-12-22
Rated 5 out of 5 by from Detection efficiency is very goodOur laboratory likes to use this antibody, Western blot, 1:200 dilution, it works well, and this antibody detection efficiency is very high.
Date published: 2016-12-21
Rated 4 out of 5 by from We use this monoclonal a lot in WB and IFWe use this monoclonal a lot in WB and IF and we have published with it. Has not failed us yet!
Date published: 2015-09-11
Rated 1 out of 5 by from Used in Western Blot application; No signalUsed in Western Blot application; No signal observed on the Blot.
Date published: 2015-05-20
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ErbB4/HER4 抗体 (C-7) is rated 4.3 out of 5 by 13.
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