Date published: 2025-9-10

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Erythropoietin/EPO 抗体 (7D10): sc-80995

3.6(5)
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说明书
  • Erythropoietin/EPO 抗体 7D10 是小鼠单克隆 IgG1Erythropoietin/EPO抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组Epo
  • Erythropoietin/EPO 抗体 (7D10) 推荐用于WB, IP 和 IF,检测human 来源的 Epo
  • 目前,我们还没有完成Erythropoietin/EPO 抗体 (7D10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Epo抗体(7D10)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测人类源性Epo蛋白。抗Epo抗体(7D10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。促红细胞生成素(Epo)是一种重要的糖蛋白,由165个氨基酸组成,主要在肾脏合成,在调节红细胞生成(红细胞形成过程)中起着至关重要的作用。这种调节在稳态条件下以及应对失血或出血时尤为重要,可确保全身的氧气输送充足。Epo通过刺激骨髓中红细胞前体的增殖和成熟来实现这一目标。Epo受体EpoR在巨核细胞、红细胞祖细胞和内皮细胞上表达,有助于Epo发挥作用。EPO水平失调可导致各种病理生理状况,例如,EPO过度表达与真性红细胞增多症有关,其特征是异常干细胞中的红细胞祖细胞不受EPO影响而生长,而EPO缺乏通常与慢性疾病性贫血有关,常见于类风湿性关节炎患者。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Erythropoietin/EPO 抗体 (7D10)

sc-80995
200 µg/ml
$316.00

Hi there! Is there available information regarding the specific epitope this antibody was raised against?

Asked by: daalopezgo
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-29

EPO(7D10) 正常腎10%中性緩衝ホルマリン24-48hr固定パラフィン包埋切片、pH9.0 EDTA, 100℃, 20min 賦活 にて全く染まりません(6例行ったが全例染まらず)。

Asked by: hiro42
お問い合わせを頂きまして、誠にありがとうございました。もし弊社の日本代理店から購入した場合、その代理店に連絡して頂き、実験条件の詳細を troubleshooting form(代理店提供)に書き込んで頂きまして、此方から改善方法のアドバイスまたは代替品を代理店を通して提供したいと思います。その他の不明点等がございましたら、またご連絡ください。 Thank you for your question. If you couldn't get the expected result, please contact the company(your local distributor in Japan), from which you purchased this product. They will offer you a technical service guide to fill in. Then you can get some advice or free replacement to optimize your experiment. Thank you.
Answered by: Technical Support 8
Date published: 2019-02-21

Can Epo (7D10) sc-80995 antibody be used for IHC(P) on rat tissue?

Asked by: ireneglez
Thank you for your question. We do not currently recommend, Epo Antibody (7D10): sc-80995, for detection of rat Epo because we have not independently validated the antibody to react with this species. The antibody is recommended for IHC(P) with human samples. We are aware of one publication citing use of the antibody for detection of rat Epo (PMID: 26398409). For a limited time, we are offering a free 10µg sample size of this antibody. Please contact our Technical Service Department if you would like to request a sample for testing. Call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-03-08

Can Epo (7D10): sc-80995 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Epo (7D10): sc-80995 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No staining in IHC on canine tissueI tested this antibody in IHC on FFPE canine tissues without any stainings.
Date published: 2021-12-28
Rated 4 out of 5 by from HiI could provide the Epo Antibody analysis certificate (7D10): sc-80995, lot: C2118.
Date published: 2019-04-02
Rated 5 out of 5 by from Excellent!This antibody is great in Western blot analysis of Epo expression in human hepatoma whole cell lysate.
Date published: 2017-02-02
Rated 4 out of 5 by from Good WB data showing strong signal at expectedGood WB data showing strong signal at expected MW with some background in human hepatoma whole cell lysate. -SCBT QC
Date published: 2015-06-18
Rated 4 out of 5 by from Produced good immunoperoxidase cytoplasmicProduced good immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-05-09
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Erythropoietin/EPO 抗体 (7D10) is rated 3.6 out of 5 by 5.
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