Date published: 2025-9-6

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ephrin-B3 抗体 (D-11): sc-390696

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  • ephrin-B3 抗体 D-11 是小鼠单克隆 IgG2a κ,ephrin-B3抗体, 在1篇文献中引用,规格为200 µg/ml
  • 与human的2-21氨基酸残基的ephrin-B3
  • ephrin-B3 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ephrin-B3
  • 抗ephrin-B3抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ephrin-B3 (D-11): sc-390696免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是ephrin-B3 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ephrin-B3 Antibody (D-11) 打包提供(请参阅下面的订购信息)。

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    関連項目

    ephrin-B3抗体(D-11)是一种IgG2a κ小鼠单克隆ephrin-B3抗体(也称为Ephrin B3抗体或EFNB3抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的ephrin-B3蛋白。ephrin-B3抗体(D-11)既可作为非偶联抗ephrin-B3抗体形式,也可作为多种偶联形式的抗ephrin-B3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Eph亚家族是目前已知的最大的一组受体蛋白激酶。越来越多的证据表明,Eph家族成员参与中枢神经系统功能及其发育。Eph受体的配体包括ephrin-A1(LERK-1/B61),被鉴定为EphA2(Eck)受体的配体;ephrin-A2(ELF-1),被鉴定为EphA3和EphA4(Sek)受体的配体;ephrin-A3(LERK-3),被鉴定为EphA5(Ehk1)和EphA3(Hek)受体的配体;ephrin-A4(LERK-4),被鉴定为EphA3受体的配体;ephrin-A5(AL-1),被鉴定为EphA5(REK7)的配体;ephrin-B1(LERK-2),被鉴定为EphB1(Elk)和EphB2(Cek5)受体的配体;ephrin-B2(LERK-5),被鉴定为EphB1、EphB3(Cek10)和EphB2受体的配体;以及ephrin-B3(LERK-8),被鉴定为EphB1的配体。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    ephrin-B3 抗体 (D-11) 参考文献:

    1. Ephrin-B3会降低成年大鼠脊髓衍生神经干/祖细胞在体外和移植到受伤大鼠脊髓后的存活率。  |  Fan, XY., et al. 2013. Stem Cells Dev. 22: 359-73. PMID: 22900481
    2. Ephrin B3 与多种 EphA 受体相互作用,推动非小细胞肺癌的迁移和侵袭。  |  Efazat, G., et al. 2016. Oncotarget. 7: 60332-60347. PMID: 27533087
    3. Ephrin-B3通过细胞间对EphBs的竞争控制兴奋性突触密度。  |  Henderson, NT., et al. 2019. Elife. 8: PMID: 30789343
    4. 与新出现的多模式中脑图谱有关的区室 Ephrin-B3 引导模式登记。  |  Stinson, JPC., et al. 2021. Front Neuroanat. 15: 649478. PMID: 33815071
    5. 尼帕病毒附着糖蛋白与宿主细胞受体Ephrin-B2和Ephrin-B3在家养和近家养哺乳动物中相互作用的硅学预测。  |  Hoque, AF., et al. 2023. Infect Genet Evol. 116: 105516. PMID: 37924857
    6. ELF-1的鉴定和克隆,ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
    7. ELF-2是Eph配体家族的一个新成员,在小鼠胚胎后脑和新形成的体节区域节段性表达。  |  Bergemann, AD., et al. 1995. Mol Cell Biol. 15: 4921-9. PMID: 7651410
    8. 克隆参与轴突束形成的 Eph 相关酪氨酸激酶受体配体 AL-1。  |  Winslow, JW., et al. 1995. Neuron. 14: 973-81. PMID: 7748564
    9. 受体酪氨酸激酶 hek 和 elk 的配体:分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
    10. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404
    11. B61 是 ECK 受体蛋白酪氨酸激酶的配体。  |  Bartley, TD., et al. 1994. Nature. 368: 558-60. PMID: 8139691
    12. Elk-L3是Eph家族受体酪氨酸激酶的一种新型跨膜配体,在胚胎底板, 顶板和后脑节段中表达。  |  Gale, NW., et al. 1996. Oncogene. 13: 1343-52. PMID: 8808709

    订购信息

    产品名称产品编号规格价格数量收藏夹

    ephrin-B3 抗体 (D-11)

    sc-390696
    200 µg/ml
    $316.00

    ephrin-B3 (D-11): m-IgG Fc BP-HRP 套装

    sc-530231
    200 µg Ab; 10 µg BP
    $354.00

    ephrin-B3 (D-11): m-IgGκ BP-HRP 套装

    sc-523642
    200 µg Ab, 40 µg BP
    $354.00

    ephrin-B3 (D-11): m-IgG2a BP-HRP 套装

    sc-547462
    200 µg Ab; 10 µg BP
    $354.00

    ephrin-B3 抗体 (D-11) AC

    sc-390696 AC
    500 µg/ml, 25% agarose
    $416.00

    ephrin-B3 抗体 (D-11) HRP

    sc-390696 HRP
    200 µg/ml
    $316.00

    ephrin-B3 抗体 (D-11) FITC

    sc-390696 FITC
    200 µg/ml
    $330.00

    ephrin-B3 抗体 (D-11) PE

    sc-390696 PE
    200 µg/ml
    $343.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 488

    sc-390696 AF488
    200 µg/ml
    $357.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 546

    sc-390696 AF546
    200 µg/ml
    $357.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 594

    sc-390696 AF594
    200 µg/ml
    $357.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 647

    sc-390696 AF647
    200 µg/ml
    $357.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 680

    sc-390696 AF680
    200 µg/ml
    $357.00

    ephrin-B3 抗体 (D-11) Alexa Fluor® 790

    sc-390696 AF790
    200 µg/ml
    $357.00

    ephrin-B3 (D-11) 中和勝肽

    sc-390696 P
    100 µg/0.5 ml
    $68.00

    What application is the blocking peptide sc-390696 P appropriate for?

    Asked by: TinTin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 3 out of 5 by from WB evaluationI tested this antibody with mouse brain cortex lysate. I got a band ~40-43kd, but I also got many other strong bands at ~23kd, ~50kd and a lot >70kd. So the specificity of this antibody was poor.
    Date published: 2017-03-09
    Rated 5 out of 5 by from Produced positive Western blot data of ephrinProduced positive Western blot data of ephrin-B3 expression in U-87 MG and Caki-1 whole cell lysates. -SCBT QC
    Date published: 2013-03-30
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    ephrin-B3 抗体 (D-11) is rated 4.0 out of 5 by 2.
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