Date published: 2025-9-6
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ephrin-B2 Antibody (F-2) - Western Blotting - Image 308154
ephrin-B2 抗体 (F-2): sc-398735. HT-29 (A), HUV-EC-C (B) 和 RT-4 (C) 全细胞裂解液中 ephrin-B2 表达的 Western 印迹分析.
ephrin-B2 抗体 (F-2): sc-398735. HT-29 (A), HUV-EC-C (B) 和 RT-4 (C) 全细胞裂解液中 ephrin-B2 表达的 Western 印迹分析.

ephrin-B2 抗体 (F-2): sc-398735

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说明书
  • ephrin-B2 抗体 F-2 是小鼠单克隆 IgG2a κ，ephrin-B2抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ephrin-B2的氨基酸168-235
  • ephrin-B2 抗体 (F-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ephrin-B2
  • 抗ephrin-B2抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ephrin-B2 (F-2): sc-398735的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是ephrin-B2 Antibody (F-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ephrin-B2 Antibody (F-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Ephrin抗体，包括ephrin-A1, ephrin-A3, ephrin-A5, ephrin-B1, ephrin-B2和ephrin-B3

ephrin-B2抗体（F-2）是一种IgG2a κ小鼠单克隆ephrin-B2抗体（也称为Ephrin B2抗体或EFNB2抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人类来源的ephrin-B2蛋白。ephrin-B2抗体（F-2）既有非偶联形式的抗ephrin-B2抗体，也有多种偶联形式的抗ephrin-B2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ephrins作为Eph受体的配体，是细胞表面蛋白，根据其结构和功能可分为ephrin-A和ephrin-B两类。Ephrin-B蛋白是跨膜蛋白，具有保守的细胞质酪氨酸残基，在与EphB受体相互作用时会被磷酸化。在胚胎发育过程中，Eph受体和ephrins在许多组织中表现出互补性表达，这表明Eph受体和ephrin-B蛋白的双向激活可能发生在表达域界面。跨膜配体ephrin-B2及其受体酪氨酸激酶EphB4分别特异性表达于动脉和静脉内皮细胞上。这两种蛋白介导的双向信号在血管发育中起着重要作用。Ephrin-B2对于胚胎血管的正常形态发生至关重要，并在肿瘤中具有血管生成作用。它已被确定为化疗治疗的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ephrin-B2 抗体 (F-2) 参考文献:

  1. Eph 受体和ephrins 限制了细胞间的交融和交流。  |  Mellitzer, G., et al. 1999. Nature. 400: 77-81. PMID: 10403252
  2. Eph 受体和ephrins  |  Jensen, PL. 2000. Stem Cells. 18: 63-4. PMID: 10661574
  3. Eph 家族受体酪氨酸激酶在致癌过程中的不同作用。  |  Nakamoto, M. and Bergemann, AD. 2002. Microsc Res Tech. 59: 58-67. PMID: 12242697
  4. ephrin-B2正向信号和EphB4反向信号在内皮细胞中的不同作用。  |  Hamada, K., et al. 2003. Arterioscler Thromb Vasc Biol. 23: 190-7. PMID: 12588758
  5. 过表达 ephrin-B2 对人类结直肠癌肿瘤生长的影响  |  Liu, W., et al. 2004. Br J Cancer. 90: 1620-6. PMID: 15083195
  6. 由ephrin-B2和EphB2介导的双向信号控制着尿直肠的发育。  |  Dravis, C., et al. 2004. Dev Biol. 271: 272-90. PMID: 15223334
  7. Ephrin-B2控制血管内皮生长因子诱导的血管生成和淋巴管生成。  |  Wang, Y., et al. 2010. Nature. 465: 483-6. PMID: 20445537
  8. 表达Ephrin-B2的自然杀伤细胞诱导血管生成  |  Wolf, KG., et al. 2022. JVS Vasc Sci. 3: 336-344. PMID: 36439700
  9. 肺动脉高压中 Ephrin-B2/EphB4 引导系统的不同作用。  |  Crnkovic, S., et al. 2023. Hypertension. 80: e17-e28. PMID: 36519465
  10. ephrin-B2配体对Eph受体和致病性鸡病毒G糖蛋白的选择性的序列基础  |  Narayanan, KK., et al. 2023. J Virol. 97: e0062123. PMID: 37931130
  11. 抑制Ephrin B2反向信号转导可消除多发性骨髓瘤的发病机制。  |  Sasine, JP., et al. 2024. Cancer Res. 84: 919-934. PMID: 38231476
  12. ephrin-B2通过EphB2-MNK-eIF4E信号传导促进小鼠和人类的痛觉可塑性和超痛觉启动  |  David, ET., et al. 2024. Pharmacol Res. 206: 107284. PMID: 38925462

订购信息

产品名称产品编号规格价格数量收藏夹

ephrin-B2 抗体 (F-2)

sc-398735
200 µg/ml
$316.00

ephrin-B2 (F-2): m-IgG Fc BP-HRP 套装

sc-526322
200 µg Ab; 10 µg BP
$354.00

ephrin-B2 (F-2): m-IgG2a BP-HRP 套装

sc-546583
200 µg Ab; 10 µg BP
$354.00

ephrin-B2 抗体 (F-2) AC

sc-398735 AC
500 µg/ml, 25% agarose
$416.00

ephrin-B2 抗体 (F-2) HRP

sc-398735 HRP
200 µg/ml
$316.00

ephrin-B2 抗体 (F-2) FITC

sc-398735 FITC
200 µg/ml
$330.00

ephrin-B2 抗体 (F-2) PE

sc-398735 PE
200 µg/ml
$343.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 488

sc-398735 AF488
200 µg/ml
$357.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 546

sc-398735 AF546
200 µg/ml
$357.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 594

sc-398735 AF594
200 µg/ml
$357.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 647

sc-398735 AF647
200 µg/ml
$357.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 680

sc-398735 AF680
200 µg/ml
$357.00

ephrin-B2 抗体 (F-2) Alexa Fluor® 790

sc-398735 AF790
200 µg/ml
$357.00

お世話になります。ephrin-B2 (F-2): sc-398735のサンプルを頂くことは可能でしょうか。

Asked by: Ayumu
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp ， www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/ 質問頂き、誠にありがとうございます。サンプル品の申請は日本のディーラーにお尋ねお願いします。ウェブサイト: www.cosmobio.co.jp ， www.iwai-chem.co.jp 又はwww.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2024-10-29

Can ephrin-B2 (F-2): sc-398735 monoclonal antibody be used to detect the extracellular fragments of EphrinB2

Asked by: niniling
Thank you for your question. The epitope for ephrin-B2 (F-2), sc-398735, maps within amino acids 168-235, however the exact epitope has not been mapped. The extracellular region for this protein is amino acids 28-229, so chances are very good the epitope is within the extracellular region.
Answered by: Tech Service
Date published: 2019-06-04

I have got sc-398735. However, there is no result in IF. Is the second antibody (such as sc-398735 AF488 and sc-398735 AF647 ) must be used?

Asked by: tun0423
Thank you for your question. It is not necessary to use the conjugated version of this mouse monoclonal primary antibody, ephrin-B2 (F-2): sc-398735, for immunofluorescence (IF). You can use a conventional anti-mouse IgG secondary antibody for detection, or one of our mouse IgG kappa light chain binding proteins (e.g., m-IgGκ BP-CFL 488: sc-516176). A link to our recommended protocol for IF with this antibody is provided below. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you still have no result with the antibody, please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com> or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-04-28

I have 2 questions: 1. Is it antibody can be used for IHC(P)? we have "Dako EnVision Dual Link System-HRP" as the second antibody. 2. If it can be used in IHC(P), which isotype control antibody is suggested? Thank u so much!

Asked by: Tun W
Thank you for your question. We have not yet tested this mouse monoclonal, ephrin-B2 Antibody (F-2): sc-398735, on formalin-fixed paraffin-embedded tissue sections, or for IHC(P) so we do not know if the antibody is suitable for this application. If you want to test the antibody for IHC(P), the antibody has an isotype of mouse monoclonal IgG2a so the corresponding isotype control is our, normal mouse IgG2a: sc-3878. https://www.scbt.com/scbt/product/normal-mouse-igg2a
Answered by: Technical Support
Date published: 2017-04-04

Can ephrin-B2 (F-2): sc-398735 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ephrin-B2 (F-2): sc-398735 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-09-05
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Rated 1 out of 5 by from not good for immune-staining in brain sectionI used this trial recently and do IF in mice brain section but not good with the solution 1:100 and 1:50. Very little signals were seen. I use this as a marker for brain arterial vessel, it should be a lot under the scope.
Date published: 2018-04-22
Rated 5 out of 5 by from 条带很清晰，也很特异，很好用。Western blot做出来的结果条带很特异，也很清晰，基本上没有杂带，几次试验下来结果都很稳定。
Date published: 2017-10-13
Rated 1 out of 5 by from This antibody doesn't work for mouse brain on WBThis antibody doesn't show band around ~37kd, only has two strong bands at size ~23kd and ~50kd for mouse brain cortex samples on WB.
Date published: 2017-03-09
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of ephrin-B2 expression in PANC-1, NIH/3T3 and NRK whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from Very good Western blot data of ephrinVery good Western blot data of ephrin-B2 expression in HT-29, HUV-EC-C and RT-4 whole cell lysates. -SCBT QC
Date published: 2014-01-17
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ephrin-B2 抗体 (F-2) is rated 3.4 out of 5 by 5.
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