Date published: 2025-9-7
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EphB2 Antibody (2D12C6) - Western Blotting - Image 362400
EphB2 抗体 (2D12C6): sc-130068. Jurkat (A), Hep G2 (B), EOC 20 (C), K-562 (D) 和 NIH/3T3 (E) 全细胞裂解液中 EphB2 表达的 Western 印迹分析.
EphB2 抗体 (2D12C6): sc-130068. Jurkat (A), Hep G2 (B), EOC 20 (C), K-562 (D) 和 NIH/3T3 (E) 全细胞裂解液中 EphB2 表达的 Western 印迹分析.

EphB2 抗体 (2D12C6): sc-130068

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寫評論提問

说明书
  • EphB2抗体(2D12C6)是小鼠单克隆IgG2b κ, 在9篇文献中引用，规格为200 µg/ml
  • 与重组蛋白结合，该重组蛋白与EphB2的17-200的氨基酸对应，来源于human
  • 抗-EphB2 抗体 (2D12C6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 EphB2
  • 抗EphB2抗体（2D12C6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EphB2 (2D12C6): sc-130068的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是EphB2 Antibody (2D12C6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与EphB2 Antibody (2D12C6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Eph抗体，包括EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphB1, EphB2, EphB3, EphB4和EphB6

EphB2抗体（2D12C6）是一种IgG2b κ小鼠单克隆EphB2抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的EphB2。EphB2抗体（2D12C6）可作为非共轭抗EphB2抗体形式提供，也可作为多种共轭形式的抗EphB2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Eph亚家族是迄今为止发现的最大一组受体蛋白酪氨酸激酶。虽然这些受体的生物活性尚未确定，但有越来越多的证据表明，它们参与中枢神经系统功能及其发育。人类来源的Eph亚家族受体（及其鼠/禽类同源物）包括EphA1（Eph）、EphA2（Eck）、EphA3（Hek4）、EphA4（Hek8）、EphA5（Hek7）、EphA6（Hek12）、EphA7（Hek11/MDK1）、EphA8（Hek3）、EphB1（Hek6）、EphB2（Hek5）、EphB3（Cek10，Hek2）、EphB4（Htk）、EphB5（Hek9）和EphB6（Mep）。Eph受体的配体包括与EphA3和EphB1结合的ephrin-A4（LERK-4）。此外，ephrin-A2（ELF-1）被描述为EphA4的配体，ephrin-A3（Ehk1-L）作为EphA5的配体，ephrin-B2（Htk-L）作为EphB4（Htk）的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EphB2 抗体 (2D12C6) 参考文献:

  1. EPHB2受体和EFNB1配体之间的相互作用通过Wnt/β-catenin/FAK途径驱动胃癌的侵袭和转移。  |  Hu, Y., et al. 2024. Int J Biol Macromol. 258: 128848. PMID: 38114003
  2. 泛素连接酶和信号枢纽 MYCBP2 是高效 EPHB2 酪氨酸激酶受体功能的必要条件。  |  Chang, C., et al. 2024. Elife. 12: PMID: 38289221
  3. 杏仁基底外侧EphB2的过表达对诱导大鼠内脏痛敏化至关重要。  |  Duan, GB., et al. 2024. CNS Neurosci Ther. 30: e14611. PMID: 38353051
  4. 全面的泛癌症分析表明，EPHB2 是预测预后和免疫疗法反应的新型生物标记物。  |  Xu, S., et al. 2024. BMC Cancer. 24: 1064. PMID: 39198775
  5. ELF-1的鉴定和克隆，ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  6. EPH相关受体蛋白酪氨酸激酶Htk配体的分子克隆。  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
  7. 鉴定 Eph 受体酪氨酸激酶家族新成员 Ehk-3 的全长和截短形式。  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
  8. 鉴定编码 MDK1（一种在小鼠神经系统中表达的新型受体酪氨酸激酶）变体的替代剪接 mRNA。  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
  9. 受体酪氨酸激酶 hek 和 elk 的配体：分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
  10. 五种人类 EPH 样受体蛋白-酪氨酸激酶的 cDNA 克隆和组织分布。  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
  11. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404
  12. 并膜酪氨酸残基将神经细胞中的 Eph 家族受体 EphB2/Nuk 与特定 SH2 结构域蛋白结合起来。  |  Holland, SJ., et al. 1997. EMBO J. 16: 3877-88. PMID: 9233798

订购信息

产品名称产品编号规格价格数量收藏夹

EphB2 抗体 (2D12C6)

sc-130068
200 µg/ml
$316.00

EphB2 (2D12C6): m-IgGκ BP-HRP 套装

sc-521206
200 µg Ab, 40 µg BP
$354.00

EphB2 (2D12C6): m-IgG2b BP-HRP 套装

sc-548872
200 µg Ab; 10 µg BP
$354.00

EphB2 抗体 (2D12C6) AC

sc-130068 AC
500 µg/ml, 25% agarose
$416.00

EphB2 抗体 (2D12C6) HRP

sc-130068 HRP
200 µg/ml
$316.00

EphB2 抗体 (2D12C6) FITC

sc-130068 FITC
200 µg/ml
$330.00

EphB2 抗体 (2D12C6) PE

sc-130068 PE
200 µg/ml
$343.00

EphB2 抗体 (2D12C6) Alexa Fluor® 488

sc-130068 AF488
200 µg/ml
$357.00

EphB2 抗体 (2D12C6) Alexa Fluor® 546

sc-130068 AF546
200 µg/ml
$357.00

EphB2 抗体 (2D12C6) Alexa Fluor® 594

sc-130068 AF594
200 µg/ml
$357.00

EphB2 抗体 (2D12C6) Alexa Fluor® 647

sc-130068 AF647
200 µg/ml
$357.00

EphB2 抗体 (2D12C6) Alexa Fluor® 680

sc-130068 AF680
200 µg/ml
$357.00

EphB2 抗体 (2D12C6) Alexa Fluor® 790

sc-130068 AF790
200 µg/ml
$357.00

お世話になります。EphB2 (2D12C6): sc-130068のサンプルを頂くことは可能でしょうか。

Asked by: Ayumu
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp ， www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/ 質問頂き、誠にありがとうございます。サンプル品の申請は日本のディーラーにお尋ねお願いします。ウェブサイト: www.cosmobio.co.jp ， www.iwai-chem.co.jp 又はwww.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2024-11-01

We used EphB2 antibody for western blot in mouse tissue sample. I expected 130kDa EphB2 band, but ~90kDa band is much stronger than 130kDa, even load 40ug of protein are loaded. Also, 130kDa band is too weak to detect. Could I get some advice about it?

Asked by: aaaaaaa
Thank you for your question. It would be helpful if you could email us directly at asia@scbio.cn. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2021-12-07

Does the Anti-EphB2 Antibody (2D12C6): sc-130068 recognize an extracellular epitope of EphB2? Does it bind to EphB2 in the membrane without permeabilization?

Asked by: JimPonce
Thank you for your question. Anti-EphB2 Antibody (2D12C6): sc-130068 was raised against a recombinant protein corresponding to amino acids 17-200 of EphB2 of human origin, which corresponds to the extracellular domain of the protein.
Answered by: Tech Support Europe
Date published: 2021-10-22

データシート上にあるウエスタンブロッティングで、バンドが2本あるんですがそれぞれのバンドはどの部分を示していますか？(切断された部位など) また、実際にウエスタンブロッティングを行った結果、約70~80kDaの部分のバンドは検出したのですが、130kDaのバンドが検出されませんでした。何か原因があるのでしょうか。

Asked by: FujitaK
Thank you for your question. Please contact our Technical Service Department at asia@scbio.cn, or your local distributors for support.
Answered by: Technical Support 12
Date published: 2021-07-07

请问这个抗体识别的是EphB2的胞外段吗？我做免疫荧光，想染胞膜，不想胞浆着色

Asked by: yangli33818
Thank you for your inquiry. EphB2 Antibody (2D12C6): sc-130068 is raised against a recombinant protein corresponding to amino acids 17-200 of EphB2 of human origin. Amino acids 19 – 543 is located at extracellular.And we can provide FREE 10 µg sample of EphB2 (2D12C6): sc-130068. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。EphB2 (2D12C6): sc-130068抗原是人重组EphB2蛋白的17-200氨基酸序列。该蛋白的19-543位于胞外域。另外我们提供10µg 的试用装，可以联系我们亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的中文支持。
Answered by: Technical Support 9
Date published: 2021-08-21

Are there any issues with using sc-130068: EphB2 (2D12C6) mouse monoclonal primary antibody on mouse samples?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-130068 conjugates.
Answered by: Technical Support
Date published: 2019-12-25
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Rated 1 out of 5 by from NO BANDSI tried this antibody in human prostate cancer metastatic cell line LNCaP even at 1:300 dilution but I did not detect any bands.
Date published: 2020-11-04
Rated 5 out of 5 by from Good staining by IFShows strong staining in A549 lung cancer cells by immunofluorescence.
Date published: 2019-06-26
Rated 4 out of 5 by from Nice resultWorked quite well. Western blot (1:300 dillution).
Date published: 2017-10-31
Rated 5 out of 5 by from Great immunofluorescenceUsed the EphB2 antibody for some Immunofluorescence experiments. Very please with results -- robust labeling and expected receptor localization
Date published: 2017-08-16
Rated 5 out of 5 by from EphB2 in brain ratsI tried the monoclonal antibody for EphB2 (sc130068). The antibody worked in rats. It is very specific to identify the difference cleavages of this protein in difference parts of neuron.
Date published: 2017-02-16
Rated 3 out of 5 by from Positive detection of the truncated humanPositive detection of the truncated human recombinant EphB6 protein and of over-expressed EphB6 protein in transfected CHO-K1 cells. -SCBT QC
Date published: 2014-06-06
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EphB2 抗体 (2D12C6) is rated 3.8 out of 5 by 6.
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