Date published: 2025-9-7
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Endoglin/CD105 Antibody (P3D1) - Western Blotting - Image 12046
Endoglin 抗体 (P3D1): sc-18838. 小鼠胚胎组织提取物中 Endoglin 表达的 Western 印迹分析.
Endoglin 抗体 (P3D1): sc-18838. 小鼠胚胎组织提取物中 Endoglin 表达的 Western 印迹分析.

Endoglin/CD105 抗体 (P3D1): sc-18838

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说明书
  • Endoglin/CD105 抗体 P3D1 是小鼠单克隆 IgG2a κ，Endoglin/CD105抗体, 在37篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Endoglin
  • Endoglin/CD105 抗体 (P3D1) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 TGF-Receptor III complex (known also as Endoglin or CD105) of Endoglin
  • 抗Endoglin/CD105抗体（P3D1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Endoglin/CD105 (P3D1): sc-18838的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Endoglin/CD105 抗体 (P3D1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

内皮素抗体（P3D1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类的内皮素蛋白。Endoglin 单克隆抗体（P3D1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。Endoglin 也称为 CD105，在血管生成中起着至关重要的作用，是血管正常形成所必需的。内皮素主要位于血管内皮细胞表面，与转化生长因子-β（TGF-β）超家族配体相互作用，促进其信号传导通路，从而调节细胞增殖、迁移和分化。内皮素在血管生物学中的重要性体现在其与遗传性出血性毛细血管扩张症（HHT）的关系上，这是一种以异常血管形成导致过度出血和其他血管并发症为特征的遗传性疾病。内皮素基因突变会破坏内皮素的功能，导致HHT1的病理生理学，从而凸显出这种蛋白质在维持血管完整性和功能方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Endoglin/CD105 抗体 (P3D1) 参考文献:

  1. 遗传性出血性毛细血管扩张症中激活素受体样激酶-1基因的新型错义突变和框架转换突变。突变简讯第 164 期。在线。  |  Klaus, DJ., et al. 1998. Hum Mutat. 12: 137. PMID: 10694922
  2. 荷属安的列斯群岛遗传性出血性毛细血管扩张症家族中两种常见的内胚叶蛋白突变：创始人效应的证据。  |  Gallione, CJ., et al. 2000. Hum Genet. 107: 40-4. PMID: 10982033
  3. 遗传性出血性毛细血管扩张症的遗传和分子发病机制。  |  Azuma, H. 2000. J Med Invest. 47: 81-90. PMID: 11019486
  4. 内切蛋白缺陷小鼠--研究遗传性出血性毛细血管扩张症的独特模型  |  Bourdeau, A., et al. 2000. Trends Cardiovasc Med. 10: 279-85. PMID: 11343967
  5. 影响转化生长因子-β1水平的修饰基因在遗传性出血性毛细血管扩张症内皮素杂合子小鼠血管缺陷发育中的潜在作用。  |  Bourdeau, A., et al. 2001. Am J Pathol. 158: 2011-20. PMID: 11395379
  6. MADH4/BMPR1A突变阴性幼年息肉病患者的ENG突变。  |  Howe, JR., et al. 2007. Clin Genet. 71: 91-2. PMID: 17204053
  7. 内皮素：心血管健康的关键介质。  |  Kapur, NK., et al. 2013. Vasc Health Risk Manag. 9: 195-206. PMID: 23662065
  8. 可溶性内切蛋白通过与αⅡbβ3整合素相互作用减少血栓形成和血小板聚集  |  Rossi, E., et al. 2023. J Thromb Haemost. 21: 1943-1956. PMID: 36990159
  9. 内皮素和可溶性内皮素在肝窦内皮功能障碍中的体内作用。  |  Eissazadeh, S., et al. 2024. Biochim Biophys Acta Mol Basis Dis. 1870: 166990. PMID: 38110128
  10. 小鼠活化素受体样激酶-1（ALK-1）同源物的克隆和特征描述。  |  Wu, X., et al. 1995. Biochem Biophys Res Commun. 216: 78-83. PMID: 7488127
  11. 人类黑色素瘤中转化生长因子 beta1 和 beta3 结合蛋白--内切蛋白（CD105）的表达和结构特征。  |  Altomonte, M., et al. 1996. Br J Cancer. 74: 1586-91. PMID: 8932339
  12. 遗传性出血性毛细血管扩张症中内胚叶蛋白基因的突变和表达分析发现了空等位基因。  |  Gallione, CJ., et al. 1998. Hum Mutat. 11: 286-94. PMID: 9554745

订购信息

产品名称产品编号规格价格数量收藏夹

Endoglin/CD105 抗体 (P3D1)

sc-18838
200 µg/ml
$316.00

Endoglin/CD105 抗体 (P3D1) AC

sc-18838 AC
500 µg/ml, 25% agarose
$416.00

Endoglin/CD105 抗体 (P3D1) HRP

sc-18838 HRP
200 µg/ml
$316.00

Endoglin/CD105 抗体 (P3D1) FITC

sc-18838 FITC
200 µg/ml
$330.00

Endoglin/CD105 抗体 (P3D1) PE

sc-18838 PE
200 µg/ml
$343.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 488

sc-18838 AF488
200 µg/ml
$357.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 546

sc-18838 AF546
200 µg/ml
$357.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 594

sc-18838 AF594
200 µg/ml
$357.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 647

sc-18838 AF647
200 µg/ml
$357.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 680

sc-18838 AF680
200 µg/ml
$357.00

Endoglin/CD105 抗体 (P3D1) Alexa Fluor® 790

sc-18838 AF790
200 µg/ml
$357.00

What is the volume per unit?

Asked by: BeaB
Thank you for your question. The volume is 1ml.
Answered by: BlakeJ
Date published: 2024-06-12

Has anyone tested this against Rat cells since it is recommended to work in rats too?

Asked by: Neha Rana
Thank you for your question. Yes, this antibody is rat reactive and it is recommended and warrantied for that use.
Answered by: Tech Service
Date published: 2019-04-16

Has anyone checked this antibody against Rat mesenchymal stem cells ?

Asked by: Neha Rana
Thank you for your question. Unfortunately we are not aware of any use specifically on rat mesenchymal stem cells, but we do know the antibody is rat reactive.
Answered by: Tech Service
Date published: 2019-04-16

What is the emission wavelength of this antibody(sc-18838 AF647)?

Asked by: yuzhizhi
Thank you for your question. The excitation for AF647 is 652nm and the emission is 668nm.
Answered by: Tech Service
Date published: 2019-01-17

I've tested a different protocol for IHC-P, but tonsil tissue as the positive control is not stained. my concern is about storage condition, I stored it in -20 for 2 months, can these storage conditions be problematic?

Asked by: SAHAR
Thank you for your question. Most of our antibodies are provided in PBS with gelatin and sodium azide. When the antibody is frozen, it precipitates out of the solution due to the presence of gelatin and may denature. We recommend storing all antibodies contained in PBS with gelatin at 4 °C. At this temperature, they are usually viable for a number of years.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: cjMara
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-02-02
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Rated 5 out of 5 by from Works great for ICC in human BMMSCs and ADMSCsBought this two months ago and it has worked perfectly with our human mesenchymal stem cells. It worked with dilutions from 1:50-1:200
Date published: 2020-03-10
Rated 1 out of 5 by from 蛍光免疫染色4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いましたが特異的なシグナルは得られませんでした。
Date published: 2018-11-26
Rated 4 out of 5 by from gooduse dilution 1: 150 over night for immunofluoresce
Date published: 2018-04-10
Rated 4 out of 5 by from goodwe use sc-18838 to detect stem cells in human intervertebral disc tissues at a concentration of 1:50, some cells were labeled.
Date published: 2017-12-14
Rated 4 out of 5 by from work goodwe've got a positve results in IHC after applicate it into hPDLCs , but the results is not what we needed
Date published: 2017-05-31
Rated 5 out of 5 by from positivePositive signal in WB in mouse embryo tissue, very stable antibody
Date published: 2017-05-07
Rated 5 out of 5 by from great featuresI bought the antibody a mouth ago,and the product shows good quality for western blot with human colon cancer cells detection,used this several times and get very clean bands
Date published: 2017-05-02
Rated 5 out of 5 by from perfect resultWe used this antibody to detect 1:800 cell lines from WB, the antibody have a good stability and specificity.
Date published: 2017-04-07
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Endoglin/CD105 抗体 (P3D1) is rated 4.5 out of 5 by 15.
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