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Egr-2 抗体 (1G5): sc-293195

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说明书
  • Egr-2 抗体 1G5 是小鼠单克隆 IgG2b κ,Egr-2抗体, 在2篇文献中引用,规格为100 µg/ml
  • 针对部分重组蛋白,其氨基酸序列在Egr-2的human起源的217-293范围内
  • 推荐用于 mouse, rat 和 human 来源的Egr-2 WB, IP 和 ELISA检测
  • 关于如何获取Egr-2 (1G5): sc-293195免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是Egr-2 Antibody (1G5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Egr-2 Antibody (1G5) 打包提供(请参阅下面的订购信息)。

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関連項目

Egr-2抗体(1G5)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Egr-2蛋白。抗Egr-2抗体(1G5)以非结合形式提供。Egr-2蛋白作为序列特异性DNA结合转录因子发挥着关键作用,主要参与细胞生长和分化过程中的基因表达调控。Egr-2与HoxA4等目标基因启动子区域中的特定DNA位点结合,主要定位于细胞核中,影响发育过程。Egr-2与先天性少突胶质细胞神经病(CHN)和德杰林-索塔综合征(DSS)有关,突出了其重要性。这两种疾病均表现为严重的神经功能缺陷和神经传导速度缓慢,原因是EGR2基因突变。人类Egr-2和鼠类Egr-1的锌指区域高度保守,同源性高达92%,这凸显了Egr-2在转录调控中的进化意义。Egr-2单克隆抗体(1G5)是研究人员研究这些神经病变分子机制以及Egr蛋白在细胞功能中更广泛影响的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Egr-2 抗体 (1G5) 参考文献:

  1. 与 Dejerine-Sottas 综合征表型相关的早期生长反应 2 基因的新型错义突变。  |  Timmerman, V., et al. 1999. Neurology. 52: 1827-32. PMID: 10371530
  2. 表达外源 PTEN 的子宫内膜癌细胞的生长和基因表达谱分析。  |  Matsushima-Nishiu, M., et al. 2001. Cancer Res. 61: 3741-9. PMID: 11325847
  3. 遗传性神经病中的 EGR2 基因突变对髓鞘基因表达具有显性抑制作用。  |  Nagarajan, R., et al. 2001. Neuron. 30: 355-68. PMID: 11394999
  4. 参与 PTEN 信号通路的两个基因 BPOZ 和 EGR2 的生长抑制作用。  |  Unoki, M. and Nakamura, Y. 2001. Oncogene. 20: 4457-65. PMID: 11494141
  5. EGR2 突变 R359W 可导致一系列 Dejerine-Sottas 神经病。  |  Boerkoel, CF., et al. 2001. Neurogenetics. 3: 153-7. PMID: 11523566
  6. Charcot-Marie-Tooth disease 1C 中的假定蛋白降解基因 LITAF/SIMPLE 发生突变。  |  Street, VA., et al. 2003. Neurology. 60: 22-6. PMID: 12525712
  7. Egr-2 和 Egr-3 是 T 细胞活化的负调控因子。  |  Safford, M., et al. 2005. Nat Immunol. 6: 472-80. PMID: 15834410
  8. 一个 Charcot-Marie-Tooth 病家族中 EGR2 R359W 和 GJB1 V136A 的两个错义突变。  |  Chung, KW., et al. 2005. Neurogenetics. 6: 159-63. PMID: 15947997
  9. 小鼠锌指基因 Krox-20 的结构, 染色体位置和表达:多种基因产物以及与原癌基因 c-fos 的核心调控。  |  Chavrier, P., et al. 1989. Mol Cell Biol. 9: 787-97. PMID: 2496302
  10. 编码具有 '锌结合指 '结构蛋白质的人类早期生长应答基因 EGR2 的分子克隆, 测序和绘图。  |  Joseph, LJ., et al. 1988. Proc Natl Acad Sci U S A. 85: 7164-8. PMID: 3140236
  11. 通过EGR2转录调节因子进行I型干扰素信号传导可增强CAR T细胞内在功能障碍。  |  Jung, IY., et al. 2023. Cancer Discov. 13: 1636-1655. PMID: 37011008
  12. 转录因子EGR2控制中枢神经系统中TH17细胞的归巢和致病性。  |  Gao, Y., et al. 2023. Nat Immunol. 24: 1331-1344. PMID: 37443284

订购信息

产品名称产品编号规格价格数量收藏夹

Egr-2 抗体 (1G5)

sc-293195
100 µg/ml
$316.00

Egr-2 (1G5): m-IgG2b BP-HRP 套装

sc-548545
100 µg Ab; 10 µg BP
$354.00

Can it be used for immunofluoresence?thank you.

Asked by: Fazlin
Thank you for your question. This Egr-2 antibody has not been tested for immunofluorescence and can not be recommended or warrantied for this use.
Answered by: Tech Service
Date published: 2019-10-23

What is the recommended storage condition for Egr-2 (1G5): sc-293195?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4°C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with human monocytes after 66-168 hrGM-CSF/IL4 driven differentiation to dendritic cells in published reasearch -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from NOT SPECIFIC SIGNALWe used this antibody 1: 100 on control iPSC-derived motor neurons fixed with PFA 4% to perform immunofluorescence assay. The signal, which should be nuclear, is observed in the cytoplasm (and absent into the nucleus).
Date published: 2022-05-04
Rated 5 out of 5 by from 很不错很开心呢,谢谢商家,下次还会光顾的。飞航感谢。产品很好产品很好产品很好产品很好产品很好产品很好产品很好
Date published: 2018-12-11
Rated 1 out of 5 by from No signal on western blot of MCF7 cell extractNo signal on western blot of MCF7 whole cell extract.
Date published: 2018-01-27
Rated 4 out of 5 by from Crisp WB bandThis antibody yielded crisp, neat bands in Western blotting with human autopsy tissue (neural). The bands, however, were higher (~75kDa) than anticipated (~50kDa).
Date published: 2017-10-16
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Egr-2抗体 (1G5) is rated 3.2 out of 5 by 5.
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