Date published: 2025-9-9
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E-Selectin/CD62E/SELE Antibody (D-7) - Western Blotting - Image 377628
E-选择素抗体 (D-7): sc-137054. Jurkat (A), F9 (B), HUV-EC-C (C), AN3 CA (D), RT-4 (E) 和 M1 (F) 全细胞裂解液中 E-Selectin 表达的 Western 印迹分析.
E-选择素抗体 (D-7): sc-137054. Jurkat (A), F9 (B), HUV-EC-C (C), AN3 CA (D), RT-4 (E) 和 M1 (F) 全细胞裂解液中 E-Selectin 表达的 Western 印迹分析.

E-Selectin/CD62E/SELE 抗体 (D-7): sc-137054

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说明书
  • E-Selectin/CD62E/SELE 抗体 D-7 是小鼠单克隆 IgG2a κ，E-Selectin/CD62E/SELE抗体, 在35篇文献中引用，规格为200 µg/ml
  • 针对氨基酸311-610，在E-Selectin的human起源的C-terminus处进行映射
  • E-Selectin/CD62E/SELE 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 E-Selectin
  • 抗E-Selectin/CD62E/SELE抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素(sc-137054 B, 200 µg/ml)偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取E-Selectin/CD62E/SELE (D-7): sc-137054的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 E-Selectin/CD62E/SELE 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与E-Selectin/CD62E/SELE 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Selectin抗体，包括E-Selectin, L-Selectin和P-Selectin

E-选择素抗体（D-7）是一种IgG2a κ小鼠单克隆E-选择素抗体（也称为选择素E抗体或CD62E抗体），它通过WB、IP、IF、IHC（P）和ELISA技术检测小鼠、大鼠和人类的E-选择素蛋白。E-选择素抗体（D-7）既可以以非偶联抗E-选择素抗体的形式存在，也可以以多种偶联形式的抗E-选择素抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。选择素，也被称为CD62抗原，构成了一个参与介导细胞与白细胞相互作用的碳水化合物结合蛋白家族。L-选择素（也被称为LECAM-1或CD62L）在大多数B细胞和初始T细胞以及大多数单核细胞、中性粒细胞和嗜酸性粒细胞上表达。L-选择素与活化的内皮细胞表达的特定碳水化合物相互作用。P-选择素（也被称为GMP-140或CD62P）在活化的血小板和内皮细胞上表达，而E-选择素（也被称为ELMA-1或CD62E）在内皮细胞上表达，它们表现出重叠的配体特异性。E-选择素由细胞因子刺激的内皮细胞表达，并被认为通过介导细胞与血管内膜的粘附而在炎症部位负责血液白细胞的积累。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E-Selectin/CD62E/SELE 抗体 (D-7) 参考文献:

  1. 小鼠和人类 E 选择素上对白细胞粘附有重要作用的物种特异性和保守表位。  |  Hammel, M., et al. 2001. Exp Cell Res. 269: 266-74. PMID: 11570819
  2. 牙周炎患者的E-选择素和L-选择素多态性。  |  Houshmand, B., et al. 2009. J Periodontal Res. 44: 88-93. PMID: 18973547
  3. 选择素配体  |  Varki, A. 1994. Proc Natl Acad Sci U S A. 91: 7390-7. PMID: 7519775
  4. 由选择素-碳水化合物相互作用介导的白细胞迁移。  |  McEver, RP., et al. 1995. J Biol Chem. 270: 11025-8. PMID: 7538108
  5. L-选择素的细胞质结构域通过α-肌动蛋白与细胞骨架蛋白相互作用：受体在微绒毛中的定位不需要与α-肌动蛋白相互作用。  |  Pavalko, FM., et al. 1995. J Cell Biol. 129: 1155-64. PMID: 7538138
  6. 选择素：血管粘附分子。  |  Tedder, TF., et al. 1995. FASEB J. 9: 866-73. PMID: 7542213
  7. 选择素与碳水化合物的相互作用以及炎症反应的启动。  |  Lasky, LA. 1995. Annu Rev Biochem. 64: 113-39. PMID: 7574477
  8. 同时缺乏 P-和 E-选择素的小鼠易受感染，造血功能发生改变。  |  Frenette, PS., et al. 1996. Cell. 84: 563-74. PMID: 8598043
  9. 血小板介导的淋巴细胞向高内皮静脉输送。  |  Diacovo, TG., et al. 1996. Science. 273: 252-5. PMID: 8662511
  10. 两种选择蛋白汇聚在硫酸盐上。白细胞粘附。  |  Rosen, SD. and Bertozzi, CR. 1996. Curr Biol. 6: 261-4. PMID: 8805242
  11. 在实验性自身免疫性脑脊髓炎中，E-选择素和 P-选择素并不参与炎症细胞跨越血脑屏障的招募。  |  Engelhardt, B., et al. 1997. Blood. 90: 4459-72. PMID: 9373256
  12. 体外 T 细胞与 ICAM-1 缺失的内皮相互作用：ICAM-1 和 ICAM-2 在 T 细胞跨内皮迁移中的重要作用。  |  Reiss, Y., et al. 1998. Eur J Immunol. 28: 3086-99. PMID: 9808177

订购信息

产品名称产品编号规格价格数量收藏夹

E-Selectin/CD62E/SELE 抗体 (D-7)

sc-137054
200 µg/ml
$316.00

E-Selectin/CD62E/SELE (D-7): m-IgG Fc BP-HRP 套装

sc-528848
200 µg Ab; 10 µg BP
$354.00

E-Selectin/CD62E/SELE (D-7): m-IgGκ BP-HRP 套装

sc-521407
200 µg Ab, 40 µg BP
$354.00

E-Selectin/CD62E/SELE (D-7): m-IgG2a BP-HRP 套装

sc-547188
200 µg Ab; 10 µg BP
$354.00

E-Selectin/CD62E/SELE 抗体 (D-7) AC

sc-137054 AC
500 µg/ml, 25% agarose
$416.00

E-Selectin/CD62E/SELE 抗体 (D-7) HRP

sc-137054 HRP
200 µg/ml
$316.00

E-Selectin/CD62E/SELE 抗体 (D-7) FITC

sc-137054 FITC
200 µg/ml
$330.00

E-Selectin/CD62E/SELE 抗体 (D-7) PE

sc-137054 PE
200 µg/ml
$343.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 488

sc-137054 AF488
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 546

sc-137054 AF546
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 594

sc-137054 AF594
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 647

sc-137054 AF647
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 680

sc-137054 AF680
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) Alexa Fluor® 790

sc-137054 AF790
200 µg/ml
$357.00

E-Selectin/CD62E/SELE 抗体 (D-7) B

sc-137054 B
200 µg/ml
$320.00

what antigen retrieval pH should be used for FFPE sections?

Asked by: PSub
Thank you for your question. You can find our recommended protocol for IHC(P) staining of tissue samples under this link. We recommend heat treatment with citrate buffer for antigen retrieval, which is indicated in the protocol as well.
Answered by: Tech Support Europe
Date published: 2022-05-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 1 out of 5 by from Nessun segnale con materiale poco concentratoTestato su campioni di fettine di cervello di topo. Risultato negativo (nessun segnale). Usati 3 ug di proteine totali. Diluizione primario 1:500 in latte 5% PBS-T (0,1% Tween).
Date published: 2021-11-30
Rated 4 out of 5 by from E-selectin antibody (D-7)Antibody works well as expected. Can recommend this product.
Date published: 2019-04-29
Rated 5 out of 5 by from immunofluorescent stainingon immunofluorescent staining, this sc-137054 E-selectin antibody worked well
Date published: 2019-04-16
Rated 5 out of 5 by from positive signalThe result shows positive signal in Jurkat and Hela whole cell in WB analysis, clear band.
Date published: 2017-02-26
Rated 5 out of 5 by from good samplsWestern blot we obtained a right band in the right molecular weight 1：800 dilution for WB.
Date published: 2017-02-12
Rated 5 out of 5 by from Clear Band and Strong SignalE-Selectin antibody has good quality in Western blot. We got a clear band and strong signal.
Date published: 2017-02-05
Rated 5 out of 5 by from Top notch antibody!This monoclonal E-Selectin Antibody (D-7) represents the best choice as it is versatile and has been validated in a broad range of species and research applications.
Date published: 2017-01-26
Rated 5 out of 5 by from Based on the analysis of antibodies to the bank anBased on the analysis of antibodies to the bank and transfection lysate
Date published: 2017-01-12
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E-Selectin/CD62E/SELE抗体 (D-7) is rated 4.4 out of 5 by 12.
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