Date published: 2025-9-8

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E-cadherin 抗体 (DECMA-1): sc-59778

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说明书
  • E-cadherin 抗体 DECMA-1 是大鼠单克隆 IgG1, 在65篇文献中引用,提供 200 µg/ml
  • 针对 mouse 起源的胚胎癌细胞系 PCC4 Aza RI
  • 抗-E-cadherin 抗体 (DECMA-1) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human 和canine 来源的 E-cadherin
  • 抗E-cadherin抗体(DECMA-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取E-cadherin (DECMA-1): sc-59778免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成E-cadherin 抗体 (DECMA-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

E-cadherin抗体(DECMA-1)是一种大鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠、人类和犬的样品中的E-cadherin。抗E-钙粘蛋白抗体(DECMA-1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。E-钙黏蛋白在细胞粘附中起着至关重要的作用,是细胞间相互作用的关键媒介,对于维持组织完整性和促进形态发生过程至关重要。E-钙黏蛋白主要位于上皮细胞的粘附连接处,与邻近的E-钙黏蛋白分子形成同源相互作用,从而促进细胞间的牢固粘附。E-钙黏蛋白的正常定位和功能对于维持上皮组织结构至关重要,失调通常与包括癌症转移在内的各种病理状况有关。包括E-cadherin在内的经典钙黏蛋白具有大的胞外结构域,其中包含多个同源重复序列,这些序列有助于黏附特性。E-cadherin的胞内结构域与细胞质蛋白(如β-catenin)相互作用,将E-cadherin与肌动蛋白细胞骨架联系起来,并在调节细胞行为的信号通路中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

E-cadherin 抗体 (DECMA-1)

sc-59778
200 µg/ml
$316.00

E-cadherin 抗体 (DECMA-1) AC

sc-59778 AC
500 µg/ml, 25% agarose
$416.00

E-cadherin 抗体 (DECMA-1) HRP

sc-59778 HRP
200 µg/ml
$316.00

E-cadherin 抗体 (DECMA-1) FITC

sc-59778 FITC
200 µg/ml
$330.00

E-cadherin 抗体 (DECMA-1) PE

sc-59778 PE
200 µg/ml
$343.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 488

sc-59778 AF488
200 µg/ml
$357.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 546

sc-59778 AF546
200 µg/ml
$357.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 594

sc-59778 AF594
200 µg/ml
$357.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 647

sc-59778 AF647
200 µg/ml
$357.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 680

sc-59778 AF680
200 µg/ml
$357.00

E-cadherin 抗体 (DECMA-1) Alexa Fluor® 790

sc-59778 AF790
200 µg/ml
$357.00

Would you have references of this particular antibody used in functional assay to inhibit human E-cadherin in vitro ?

Asked by: JeromeGR
Thank you for your question. Our antibodies are designed for and tested in detection assays. We do not have data on their blocking properties.
Answered by: Tech Support Europe
Date published: 2025-01-17

Where does this antibody bind on e cadherin protein? 

Asked by: Elenachris
Thank you for your question. Anti-E-cadherin Antibody (DECMA-1): sc-59778 was raised against embryonal carcinoma cell line PCC4 Aza RI of mouse origin. At this point no epitope mapping has been performed for this antibody yet, so its exact binding site on its target protein is not known.
Answered by: Tech Support Europe
Date published: 2021-10-26

I need to use E-cadherin (DECMA-1): sc-59778 to detect E-cadherin in canine samples by Western blot. Do you know if this mouse monoclonal antibody will work?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from Works well for IF on mouse lung frozen sectionsThis antibody is cheaper and comparable in signal specificity and intensity to the rat anti-E-cadherin antibody (clone ECCD; catalog # 13-1900; $450 for 0.1mg) sold by Thermo Fisher/Invitrogen. Unfortunately, similar to the Invitrogen antibody, this antibody doesn't work for immunofluorescence on paraffin wax sections. I'll be using this antibody from now on.
Date published: 2024-01-20
Rated 4 out of 5 by from Good for WBI got as a free sample from the company. It works well for WB.
Date published: 2021-02-11
Rated 1 out of 5 by from 免疫組織化学では機能しないE-cadherinは精巣の未分化型精祖細胞に発現するとされているが、この抗体を使ってパラフィン切片を蛍光染色してもE-cadherinを検出できなかった。
Date published: 2021-01-29
Rated 5 out of 5 by from Good por IFGreat antibody for IF on MCDK cells. Dilution 1:100. Very specific signal in the plasma membrane
Date published: 2018-10-11
Rated 4 out of 5 by from Great productI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of 5 by from Great western blot antibodyThis antibody works great on whole cell lysate prepared from mouse cecum, it detects the 120kDa band (full length of E-cadherin) and also a fragment that is ~80kDa (could be the processed E-cadherin present in the sample)
Date published: 2017-11-22
Rated 5 out of 5 by from Good for IFWorked well for IF in AML12 cells, but not in MCF7
Date published: 2017-04-12
Rated 5 out of 5 by from Excellent antibodyOutstanding ihc staining results; also works well for wb applications
Date published: 2017-01-02
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E-cadherin 抗体 (DECMA-1) is rated 4.5 out of 5 by 14.
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