Date published: 2025-9-9
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
E-cadherin Antibody (67A4) - Western Blotting - Image 348906
E-cadherin 抗体 (67A4): sc-21791. A431 全细胞裂解液中 E-cadherin 表达的 Western 印迹分析.
E-cadherin 抗体 (67A4): sc-21791. A431 全细胞裂解液中 E-cadherin 表达的 Western 印迹分析.

E-cadherin 抗体 (67A4): sc-21791

3.8(31)
寫評論提問

说明书
  • E-cadherin抗体(67A4)是小鼠单克隆IgG1 κ, 在215篇文献中引用，规格为200 µg/ml
  • 针对human乳腺癌细胞系T471
  • E-cadherin 抗体 (67A4) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 E-cadherin
  • 抗E-cadherin抗体（67A4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取E-cadherin (67A4): sc-21791的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是E-cadherin Antibody (67A4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与E-cadherin Antibody (67A4) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cadherin抗体，包括cadherin-16, cadherin-19, cadherin-23, E-cadherin, EY-cadherin, K-cadherin, LI-cadherin, M-cadherin, N-cadherin, OB-cadherin, P-cadherin, pan-cadherin, R-cadherin, T-cadherin和VE-cadherin

E-钙黏蛋白抗体（67A4）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类E-钙黏蛋白。抗E-钙粘蛋白抗体（67A4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。E-钙粘蛋白是钙粘蛋白家族的一员，在细胞粘附中起着至关重要的作用，对于维持组织完整性和促进发育过程中的形态发生过程至关重要。E-钙黏蛋白主要定位于细胞膜，形成连接相邻细胞的黏附连接，有助于组织结构的稳定性。这种膜定位使E-钙黏蛋白能够调节细胞间牢固的黏附，这对于上皮层的形成和防止癌细胞的侵袭行为是必不可少的。E-钙黏蛋白与细胞质蛋白（如β-catenin）的相互作用对于调节细胞增殖和分化的信号通路至关重要，这凸显了其在正常生理和疾病状态下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

E-cadherin 抗体 (67A4)

sc-21791
200 µg/ml
$316.00

E-cadherin (67A4): m-IgG Fc BP-HRP 套装

sc-528370
200 µg Ab; 10 µg BP
$354.00

E-cadherin (67A4): m-IgGκ BP-HRP 套装

sc-520732
200 µg Ab, 40 µg BP
$354.00

E-cadherin (67A4): m-IgG1 BP-HRP 套装

sc-542898
200 µg Ab; 20 µg BP
$354.00

E-cadherin 抗体 (67A4) AC

sc-21791 AC
500 µg/ml, 25% agarose
$416.00

E-cadherin 抗体 (67A4) HRP

sc-21791 HRP
200 µg/ml
$316.00

E-cadherin 抗体 (67A4) FITC

sc-21791 FITC
200 µg/ml
$330.00

E-cadherin 抗体 (67A4) PE

sc-21791 PE
200 µg/ml
$343.00

E-cadherin 抗体 (67A4) Alexa Fluor® 488

sc-21791 AF488
200 µg/ml
$357.00

E-cadherin 抗体 (67A4) Alexa Fluor® 546

sc-21791 AF546
200 µg/ml
$357.00

E-cadherin 抗体 (67A4) Alexa Fluor® 594

sc-21791 AF594
200 µg/ml
$357.00

E-cadherin 抗体 (67A4) Alexa Fluor® 647

sc-21791 AF647
200 µg/ml
$357.00

E-cadherin 抗体 (67A4) Alexa Fluor® 680

sc-21791 AF680
200 µg/ml
$357.00

E-cadherin 抗体 (67A4) Alexa Fluor® 790

sc-21791 AF790
200 µg/ml
$357.00

Dear Santa Cruz, I am sending this message to ask if this antibody has been tested on mouse samples i.e. mouse reactivity. In addition, I read a lot of reviews with unsatisfactory results. Is there a reason? Thank you for getting back to me, Patrick

Asked by: patrikaouad
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-25

Dear Sir/Madame, I am writing this letter because I would like to try your product with the next specification: E-cadherin Antibody (67A4) Alexa Fluor® 647 sc-21791 AF647 200 µg/ml. Would you be so kind as to send me a trial size? Best regards, Babi

Asked by: Babi
Thank you for your question. We do not offer sample sizes of the conjugated primary antibodies. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this unconjugated monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-12

Is it possible to get free sample for testing before I order the antibody?

Asked by: Sammy123
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2024-05-27

Dear All Would it be possible to have sample of this antibody to test? Thank you

Asked by: fafv
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of this mouse monoclonal, sc-21791: E-cadherin (67A4). Please contact a Technical Service Representative to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-16

Can I use the E-cadherin Antibody (67A4) for detection in bovine tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_21791, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 118ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Westerblot/ImmunofluorescenceAntibody works very well in Westerblot and immunofluorescence (1:200, or 1:100 dilution, respectively) in airway epithelial cell lysate, or airway epithelial cell monolayers, respectively.
Date published: 2023-02-06
Rated 4 out of 5 by from It works!This antibody gave a strong and clean signal when I used it at a 1:200 diluition after o.n. incubation with 80 μg of proteins.
Date published: 2022-03-18
Rated 1 out of 5 by from Not goodI used the primary antibody 1:50 by IF on epithelial renal cells. Unfortunately, the signal was not detectable.
Date published: 2022-02-08
Rated 1 out of 5 by from Anticorpo non funzionantePrimary antibodies E-Cadherin (67A4) sc-21791 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membrane of PVDF has been incubated overnight at 4°C and then it has been probed for 1h at RT with Santa Cruz secondary antibodies diluted 1:5000 in Milk 1%. We have tested this antibody on two different sets of samples: in six cancer cell lines and in human activated PBMCs. Moreover, even if we tested the antibody at higher concentration, we only obtained a higher background but not an increase in the specific protein signal; therefore we show only the blots obtained after the incubation with antibody prepared as described above. In conclusion: • E-Cadherin did not work at all, neither after a long exposure. We do not have tools to verify the specificity for this antibody too. All the membranes have been probed with a normalizer and each gel has been stained with Coomassie Brillant Blue, shown on the right side of each panel, in order to demonstrate that the samples ran well and that the proteins are present on the SDS-PAGE.
Date published: 2019-11-26
Rated 5 out of 5 by from Greatit is useful to our purpose and efficiency.
Date published: 2019-10-17
Rated 1 out of 5 by from Not working at allThis antibody does not work in even 1:100 concentration neither in WB nor IF
Date published: 2018-11-30
Rated 1 out of 5 by from FACSThis antibody does not work on HFFF cells in flow cytometry.
Date published: 2018-11-01
Rated 1 out of 5 by from Not workingDoes not work in western and IF of breast cancer cell lines.
Date published: 2018-10-12
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_21791, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
E-cadherin 抗体 (67A4) is rated 3.8 out of 5 by 31.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_21791, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 110ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。