Date published: 2025-9-7
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Doublecortin/DCX Antibody (E-6) - Immunofluorescence - Image 157373
双皮质素抗体 (E-6): sc-271390. 小鼠海马 (福尔马林固定, 石蜡), 用 sc-271390；1:50 与 AlexaFluor-568 (红色) 过夜染色. 绿色-活性 Caspase-3, 蓝色-DAPI. 由雪松-西奈医学中心的 Svetlana Zonis 博士友情提供.
双皮质素抗体 (E-6): sc-271390. 小鼠海马 (福尔马林固定, 石蜡), 用 sc-271390；1:50 与 AlexaFluor-568 (红色) 过夜染色. 绿色-活性 Caspase-3, 蓝色-DAPI. 由雪松-西奈医学中心的 Svetlana Zonis 博士友情提供.

Doublecortin/DCX 抗体 (E-6): sc-271390

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说明书
  • Doublecortin/DCX 抗体 E-6 是小鼠单克隆 IgG1 κ，Doublecortin/DCX抗体, 在141篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列81-365位于human物种的Doublecortin的C-terminus
  • Doublecortin/DCX 抗体 (E-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Doublecortin
  • 抗Doublecortin/DCX抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Doublecortin/DCX (E-6): sc-271390的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Doublecortin/DCX 抗体 (E-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Doublecortin/DCX 抗体 (E-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Doublecortin抗体（E-6）是一种IgG1 κ小鼠单克隆Doublecortin抗体（也称为DCX抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的Doublecortin蛋白。Doublecortin抗体（E-6）既可以是非偶联的抗Doublecortin抗体形式，也可以是多种偶联形式的抗Doublecortin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。无脑畸形（光滑脑）是一种脑发育异常，其特征是不完全的神经元迁移和光滑的脑表面，导致严重的智力迟钝。遗传分析鉴定出两种在某些无脑畸形病例中发生突变的蛋白质，称为无脑畸形-1蛋白（LIS1）和doublecortin。LIS1与异三聚体G蛋白的β亚基具有序列同源性。doublecortin包含一个Abl磷酸化位点，与预测的激酶蛋白具有一些序列同源性。这两种蛋白质在发育中的大脑中高度表达，表明它们可能参与对脑发育至关重要的信号转导通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Doublecortin/DCX 抗体 (E-6) 参考文献:

  1. 双皮质素在识别神经丝蛋白细胞质域中的磷酸化-FIGQY酪氨酸方面的新活性。  |  Kizhatil, K., et al. 2002. J Neurosci. 22: 7948-58. PMID: 12223548
  2. 发育中大鼠视网膜中双皮质素的差异表达和细胞定位。  |  Lee, EJ., et al. 2003. Eur J Neurosci. 17: 1542-8. PMID: 12752371
  3. 双皮质蛋白在中枢和周围神经系统肿瘤以及人类非神经组织中的表达。  |  Bernreuther, C., et al. 2006. Acta Neuropathol. 111: 247-54. PMID: 16520969
  4. 双皮质素在调节微管细胞骨架方面的独特作用。  |  Moores, CA., et al. 2006. EMBO J. 25: 4448-57. PMID: 16957770
  5. 在大鼠视网膜的水平细胞中，双皮质蛋白的表达持续到成年期。  |  Wakabayashi, T., et al. 2008. Neurosci Lett. 442: 249-52. PMID: 18647639
  6. 人类双皮质素C-和N-末端结构域与特异性抗体的复合物的晶体结构。  |  Burger, D., et al. 2016. J Biol Chem. 291: 16292-306. PMID: 27226599
  7. 分离出含有类似 G 蛋白 beta-亚基重复序列的米勒-迪克麻痹基因。  |  Reiner, O., et al. 1993. Nature. 364: 717-21. PMID: 8355785
  8. 带有 WD 重复序列的蛋白质的折叠：将 WD 重复序列超家族的六个成员与 G 蛋白 beta 亚基进行比较。  |  Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
  9. 血小板活化因子乙酰水解酶的表达和活性表明，神经元迁移与血小板活化因子之间存在联系。  |  Albrecht, U., et al. 1996. Dev Biol. 180: 579-93. PMID: 8954729
  10. 神经元迁移所需的新型中枢神经系统基因，与 X 连锁皮层下异位症和无脑畸形综合征有关。  |  des Portes, V., et al. 1998. Cell. 92: 51-61. PMID: 9489699
  11. 双皮质素是一种脑特异性基因，在人类 X 连锁无脑畸形和双皮质综合征中发生突变，编码一种假定的信号蛋白。  |  Gleeson, JG., et al. 1998. Cell. 92: 63-72. PMID: 9489700
  12. LISsen up！  |  Walsh, CA. 1998. Nat Genet. 19: 307-8. PMID: 9697681

订购信息

产品名称产品编号规格价格数量收藏夹

Doublecortin/DCX 抗体 (E-6)

sc-271390
200 µg/ml
$316.00

Doublecortin/DCX (E-6): m-IgG Fc BP-HRP 套装

sc-527240
200 µg Ab; 10 µg BP
$354.00

Doublecortin/DCX (E-6): m-IgG1 BP-HRP 套装

sc-532613
200 µg Ab; 20 µg BP
$354.00

Doublecortin/DCX 抗体 (E-6) AC

sc-271390 AC
500 µg/ml, 25% agarose
$416.00

Doublecortin/DCX 抗体 (E-6) HRP

sc-271390 HRP
200 µg/ml
$316.00

Doublecortin/DCX 抗体 (E-6) FITC

sc-271390 FITC
200 µg/ml
$330.00

Doublecortin/DCX 抗体 (E-6) PE

sc-271390 PE
200 µg/ml
$343.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 488

sc-271390 AF488
200 µg/ml
$357.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 546

sc-271390 AF546
200 µg/ml
$357.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 594

sc-271390 AF594
200 µg/ml
$357.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 647

sc-271390 AF647
200 µg/ml
$357.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 680

sc-271390 AF680
200 µg/ml
$357.00

Doublecortin/DCX 抗体 (E-6) Alexa Fluor® 790

sc-271390 AF790
200 µg/ml
$357.00

I've used DCX antibody E-6 and C-18 both. I've checked the DCX signal like branch shape in C-18 antibody, but I couldn't on E-6. so, i want to see some more pictures of DCX signal in brain. may I ?

Asked by: YAMKOOKIE
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-11-01

Do I need to use a "MOM" kit if using this product on mouse brain tissue?

Asked by: lee lee
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-13

Is this antobody suitable for vibratome sections (not frozen)? Thank you !

Asked by: mmidroit
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

There was a mistake on the recipient and they put the Doublecortin Antibody (E-6) sc-271390 at -20 degrees for 3 hours. Does this affect the quality of the antibody or should it be fine?

Asked by: Anonymous
Thank you for your question. We do not recommend freezing our antibodies because they contain a small percentage of gelatin in the buffer as a stabilizing protein. Upon freezing, this can compromise the quality of the antibodies. However, if it was only frozen for 3 hours then we would still recommend testing the antibody because it may not have been compromised in such a short time.
Answered by: Technical Support
Date published: 2020-07-16

Why in the western blot image for mouse postnatal brain sample we see so many bands for doublecortin?

Asked by: Karishma
Thank you for your question, and for pointing attention to this image. Doublecortin is a hightly phosphorylated protein and in some samples it will detect post-translational modifications if they are present in the sample. We will take a look at this, it's a really strong and specific antibody for this target.
Answered by: Tech Service
Date published: 2020-02-17

Hello, please does this antibody react with avian species? Am working on Neurogenesis on the QUAIL brain. I saw that the older antibody goat anti-DCX C-18: SC-8066 react with avain species. DOES the goat anti-DCX (E-6): sc-271390 reacts with avians

Asked by: asamcy
Thank you for your question. Unfortunately we have not tested on bird samples, so it is not recommended or warrantied for that use. That said, we do have a publication in avian samples (chick, PMID: 25086319), so it very well may work fine for Quail samples.
Answered by: Tech Service
Date published: 2019-08-12

I wish to use this antibody (Doublecortin Antibody (E-6): sc-271390) against avian species. Can I get the information regarding the peptide sequence against which this antibody is designed?

Asked by: Pooja1
Thank you for your question. Unfortunately the exact epitope of this antibody has not been mapped, so I do not have that information. We do have a previous publication in avian samples (PMID: 25086319), maybe that is helpful for you.
Answered by: Tech Service
Date published: 2019-04-18

I am going to use Doublecortin (sc-271390 ) for IHC. my queries are 1. Will this be suitable for both IHC (paraformaldehyde fixed frozen sections) and Western blotting? 2. Could you suggest me a compatible secondary for this primary?

Asked by: Senthil
Thank you for your question. Yes, this antibody is recommended for both IHC on frozen sections and Western blot, so it is a great choice. I would recommend our new Mouse IgG binding protein for both WB and IHC, you can find them here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry These are optimzed for these applications and we have had excellent results with them. We would be happy to send you samples of both the primary and the secondary reagents if you would like to try them! Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com for any sample requests or questions.
Answered by: Tech Service
Date published: 2017-05-29
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Rated 4 out of 5 by from きれいに染色できましたラットの脳を用い、免疫組織化学染色 (IHC(P)) を行いました。 きれいに染色できたため今後も使用を検討したいと思います。
Date published: 2024-07-06
Rated 4 out of 5 by from Some lotes doesn't work!!!I bought a vial and it did work (lot C0118). A sample of the same cat. number given by a colleague worked fantastic. Just a comment on the picture you show, the red signal is mainly autofluorescence of blood. Attached is an image we obtained with the sample our colleague kindly gave us.
Date published: 2023-08-28
Rated 5 out of 5 by from Excellent customer feedback.Express delivery in a week period. Proper handling. Thank you.
Date published: 2022-07-28
Rated 4 out of 5 by from きれいサンプル使用しました。蛍光免疫染色で細胞の染色がきれいにできました。また使用を検討したいと思います。
Date published: 2021-08-30
Rated 3 out of 5 by from It workedI used this antibody for mice brain IHC. It worked but the signal was not so srong.
Date published: 2021-06-01
Rated 4 out of 5 by from Nice signal for Western blotThis antibody works nicely on Western blot samples from rat hippocampus. A clear double-band can be detected between 37 and 50 kDa, corresponding to the expected molecular weight of the protein. Antibody dilution: 1:500 in PBST with 5% nonfat dry milk.
Date published: 2021-02-17
Rated 4 out of 5 by from Für Kryoschnitte geeignetGute Färbeergebnisse, allerdings musste eine 1:20 Verdünnung eigesetzt werden. Klon E-5 brachte keine Ergebnisse.
Date published: 2020-10-15
Rated 4 out of 5 by from Immunohistochemistryで使用しました。マウスの脳切片を使用して、Immunohistochemistryで検出を行いました。感度よく検出できたと思います。
Date published: 2020-08-05
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Doublecortin/DCX 抗体 (E-6) is rated 4.3 out of 5 by 39.
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