Doublecortin/DCX Antibody (E-5): sc-390645

Doublecortin Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Doublecortin, a pivotal microtubule-associated protein essential for neuronal migration and brain development, across multiple species including mouse, rat, human, equine, canine, bovine, and porcine. Anti-Doublecortin antibody (E-5) targets a precise epitope mapping between amino acids 46-79 at the N-terminus of human Doublecortin, ensuring accurate recognition and minimal cross-reactivity. Doublecortin, also called DC, DCX, DBCN, LISX, SCLH, and XLIS, has a structure characterized by two tandem Doublecortin (DCX) domains, each responsible for binding to microtubules and stabilizing their polymerization. These structural domains are crucial for maintaining the dynamic instability of microtubules, which is essential for proper migration of neurons during cerebral cortex formation. Doublecortin′s ability to interact with tubulin dimers and enhance microtubule dynamics is fundamental for structural integrity and flexibility required for neuronal processes and synaptic plasticity. Disruptions in Doublecortin′s structural integrity, often resulting from genetic mutations, are directly linked to neurodevelopmental disorders such as lissencephaly, characterized by impaired neuronal migration and severe brain malformations. Doublecortin monoclonal antibody (E-5) is optimized for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), and is available in both non-conjugated form and multiple conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. By enabling precise detection and analysis of Doublecortin′s structural domains and their interactions with the cytoskeleton, Doublecortin (E-5) antibody facilitates advanced research in neuroscience, developmental biology, and molecular mechanisms underlying neurological disorders. Anti-Doublecortin antibody (E-5) is an invaluable tool for scientists aiming to elucidate the intricate processes of brain development and the pathophysiology of related diseases, thereby contributing to the development of targeted therapeutic strategies.

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Doublecortin/DCX Antibody (E-5) References:

1. Doublecortin, a stabilizer of microtubules.  |  Horesh, D., et al. 1999. Hum Mol Genet. 8: 1599-610. PMID: 10441322
2. Doublecortin interacts with mu subunits of clathrin adaptor complexes in the developing nervous system.  |  Friocourt, G., et al. 2001. Mol Cell Neurosci. 18: 307-19. PMID: 11591131
3. The DCX-domain tandems of doublecortin and doublecortin-like kinase.  |  Kim, MH., et al. 2003. Nat Struct Biol. 10: 324-33. PMID: 12692530
4. Doublecortin functions at the extremities of growing neuronal processes.  |  Friocourt, G., et al. 2003. Cereb Cortex. 13: 620-6. PMID: 12764037
5. The DC-module of doublecortin: dynamics, domain boundaries, and functional implications.  |  Cierpicki, T., et al. 2006. Proteins. 64: 874-82. PMID: 16835924
6. The doublecortin gene family and disorders of neuronal structure.  |  Dijkmans, TF., et al. 2010. Cent Nerv Syst Agents Med Chem. 10: 32-46. PMID: 20236041
7. Domain swap in the C-terminal ubiquitin-like domain of human doublecortin.  |  Rufer, AC., et al. 2018. Acta Crystallogr D Struct Biol. 74: 450-462. PMID: 29717716
8. Lack of MeCP2 leads to region-specific increase of doublecortin in the olfactory system.  |  Martínez-Rodríguez, E., et al. 2019. Brain Struct Funct. 224: 1647-1658. PMID: 30923887
9. Expression and chromosomal localization of KIAA0369, a putative kinase structurally related to Doublecortin.  |  Omori, Y., et al. 1998. J Hum Genet. 43: 169-77. PMID: 9747029
10. Cloning and developmental expression of the murine homolog of doublecortin.  |  Matsuo, N., et al. 1998. Biochem Biophys Res Commun. 252: 571-6. PMID: 9837748

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Doublecortin/DCX Antibody (E-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Doublecortin/DCX Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Doublecortin/DCX Antibody (E-5) Specifications

Alternate Product Name: Doublecortin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 46-79

Epitope Target: Doublecortin

Epitope Species: human

Product Reactivity/Detection: Doublecortin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Doublecortin/DCX Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Doublecortin/DCX antibody is Doublecortin/DCX Antibody (E-6): sc-271390.