Date published: 2025-9-6

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DNA pol α 抗体 (D-4): sc-373884

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说明书
  • DNA pol α抗体(D-4)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的DNA pol α的N-terminus附近的氨基酸3-31之间
  • 推荐用于 mouse, rat 和 human 来源的DNA pol α WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是DNA pol α Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DNA pol α Antibody (D-4) 打包提供(请参阅下面的订购信息)。

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関連項目

DNA的复制、重组和修复是维持基因组稳定性所必需的过程,这些过程需要核酸外切酶的参与。在DNA复制过程中,这些酶参与冈崎片段的加工;而在DNA修复过程中,它们的功能是切除受损的DNA片段并纠正重组错配。这些核酸外切酶包括DNA聚合酶家族。DNA聚合酶α、β、δ和ε参与DNA的复制和修复。DNA聚合酶δ和DNA聚合酶ε是多亚基酶,其中DNA聚合酶δ由两个亚基组成:p125,它与滑动DNA夹蛋白PCNA相互作用;另一个是p50。核编码的DNA聚合酶γ是线粒体DNA复制所需的唯一DNA聚合酶。DNA聚合酶ζ在各种组织中普遍表达,并介导损伤诱导突变的细胞机制。DNA聚合酶θ是一种与ATP结合的DNA聚合酶-解旋酶,并参与链间交联的修复。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNA pol α 抗体 (D-4) 参考文献:

  1. 小鼠DNA聚合酶α-引物酶复合物的分子结构。  |  Mizuno, T., et al. 1999. Mol Cell Biol. 19: 7886-96. PMID: 10523676
  2. 玉米DNA聚合酶α由PCNA相关细胞周期蛋白/细胞周期蛋白依赖性激酶复合物磷酸化:苯乙胺的影响。  |  Gómez Roig, E. and Vázquez-Ramos, JM. 2003. J Plant Physiol. 160: 983-90. PMID: 14593798
  3. DNA聚合酶α的错误由DNA聚合酶δ纠正的证据。  |  Pavlov, YI., et al. 2006. Curr Biol. 16: 202-7. PMID: 16431373
  4. Mcm10 和 And-1/CTF4 将 DNA 聚合酶 alpha 募集到染色质,以启动 DNA 复制。  |  Zhu, W., et al. 2007. Genes Dev. 21: 2288-99. PMID: 17761813
  5. DNA聚合酶α与复制SV40核蛋白复合物有关。  |  Otto, B. and Fanning, E. 1978. Nucleic Acids Res. 5: 1715-28. PMID: 208060
  6. DNA聚合酶α/引物酶辅助蛋白的纯化和特性。  |  Goulian, M., et al. 1990. J Biol Chem. 265: 13221-30. PMID: 2165497
  7. HeLa DNA聚合酶α暴露于蛋白激酶C会影响其催化特性。  |  Krauss, SW., et al. 1987. J Biol Chem. 262: 3432-5. PMID: 3818650
  8. 通过用特定的刺激辅助因子C1C2重组DNA聚合酶α来制备DNA聚合酶αX C1C2。  |  Pritchard, CG. and DePamphilis, ML. 1983. J Biol Chem. 258: 9801-9. PMID: 6885771
  9. 重组 DNA 聚合酶-α-primase 在体外复制 DNA。  |  Stadlbauer, F., et al. 1994. Eur J Biochem. 222: 781-93. PMID: 8026492
  10. 细胞周期中DNA聚合酶α分布的超微结构。  |  Lattanzi, G., et al. 1998. J Histochem Cytochem. 46: 1435-42. PMID: 9815285

订购信息

产品名称产品编号规格价格数量收藏夹

DNA pol α 抗体 (D-4)

sc-373884
200 µg/ml
$316.00

DNA pol α (D-4): m-IgG Fc BP-HRP 套装

sc-537880
200 µg Ab; 10 µg BP
$354.00

DNA pol α (D-4): m-IgGκ BP-HRP 套装

sc-535327
200 µg Ab; 40 µg BP
$354.00

DNA pol α (D-4): m-IgG1 BP-HRP 套装

sc-545425
200 µg Ab; 20 µg BP
$354.00

DNA pol α (D-4) 中和勝肽

sc-373884 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373884 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size inin Jurkat nuclear extract. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from GreatGreat, strong band in WB in HeLa, Jurkat and K-562 nuclear extracts. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear stainingExceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2014-06-26
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DNA pol α 抗体 (D-4) is rated 5.0 out of 5 by 3.
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