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CCNB1/cyclin B1 抗体 (V152): sc-53236

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说明书
  • CCNB1/cyclin B1抗体(V152)是小鼠单克隆IgG1 κ, 在11篇文献中引用,规格为200 µg/ml
  • 针对His标签重组蛋白hamster cyclin B1的
  • 推荐用于 mouse, rat, human 和 hamster 来源的cyclin B1 WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CCNB1/cyclin B1 (GNS1): sc-245,可查看有标记的 CCNB1/cyclin B1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CCNB1/cyclin B1 Antibody (V152) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与CCNB1/cyclin B1 Antibody (V152) 打包提供(请参阅下面的订购信息)。
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関連項目

在真核细胞中,有丝分裂是由一种被称为促成熟因子、M期特异性组蛋白激酶或M期激酶的蛋白激酶激活后开始的。这种蛋白激酶由催化亚基(Cdc2)、调节亚基(cyclin B)和低分子量亚基(p13-Suc 1)组成。Cdc/cyclin酶受到多种水平的控制,其中由酪氨酸磷酸化调节催化亚基的调控是最好理解的。酪氨酸磷酸化抑制Cdc2/cyclin B酶;在细胞分裂开始时发生的酪氨酸去磷酸化直接激活了预MPF复合物。证据表明,B型cyclin不仅对M期调节亚基的Cdc2蛋白激酶有作用,而且还激活内源性酪氨酸磷酸酶Cdc25A和Cdc25B,其中Cdc2是生理底物。这种效应的特异性表现为cyclin A或cyclin D1无法显示任何对Cdc25A或Cdc25B的刺激。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CCNB1/cyclin B1 抗体 (V152) 参考文献:

  1. 细胞色素B1和其他细胞色素作为肿瘤抗原在癌症免疫监测和免疫治疗中的作用。  |  Egloff, AM., et al. 2006. Cancer Res. 66: 6-9. PMID: 16397206
  2. 细胞周期蛋白A2调节核膜破裂和细胞周期蛋白B1的核内积累。  |  Gong, D., et al. 2007. Curr Biol. 17: 85-91. PMID: 17208191
  3. 中心体分离后,细胞周期蛋白 B1-Cdk1 继续激活,控制有丝分裂进程。  |  Lindqvist, A., et al. 2007. PLoS Biol. 5: e123. PMID: 17472438
  4. 细胞质多腺苷酸化酶结合蛋白调节星形胶质细胞中细胞周期蛋白 B1 的表达  |  Kim, KC., et al. 2011. J Neurosci. 31: 12118-28. PMID: 21865454
  5. 细胞周期蛋白B1在胰腺癌中的预后价值。  |  Zhou, L., et al. 2014. Med Oncol. 31: 107. PMID: 25106528
  6. MASTL 通过加强不依赖于 Cdc20 的细胞周期蛋白 B1 与 APC/C 的结合,促进细胞周期蛋白 B1 的破坏。  |  Voets, E. and Wolthuis, R. 2015. Biol Open. 4: 484-95. PMID: 25750436
  7. CYCLIN-B1/2和-D1在协调大脑皮层祖细胞自我更新和血统承诺方面起着相反的作用。  |  Hagey, DW., et al. 2020. Nat Commun. 11: 2898. PMID: 32518258
  8. 氧化应激诱导的 CDK1-Cyclin B1 计划外活动损害 ER 线粒体介导的生物能量代谢  |  Chang, JG., et al. 2021. Cells. 10: PMID: 34064109
  9. 巨噬细胞通过TNF-α-PI3K-Cdk1/Cyclin B1轴维持乳腺干细胞活性和乳腺稳态。  |  Zhou, Y., et al. 2023. NPJ Regen Med. 8: 23. PMID: 37130846
  10. 对人类肿瘤中细胞周期蛋白B1(CCNB1)的致癌作用进行全癌分析。  |  Dai, P., et al. 2023. Sci Rep. 13: 16226. PMID: 37758792

订购信息

产品名称产品编号规格价格数量收藏夹

CCNB1/cyclin B1 抗体 (V152)

sc-53236
200 µg/ml
$322.00

CCNB1/cyclin B1 (V152): m-IgG Fc BP-HRP 套装

sc-536913
200 µg Ab; 10 µg BP
$361.00

CCNB1/cyclin B1 (V152): m-IgGκ BP-HRP 套装

sc-534086
200 µg Ab; 40 µg BP
$361.00

CCNB1/cyclin B1 (V152): m-IgG1 BP-HRP 套装

sc-544894
200 µg Ab; 20 µg BP
$361.00

CCNB1/cyclin B1 抗体 (V152) FITC

sc-53236 FITC
200 µg/ml
$336.00

CCNB1/cyclin B1 抗体 (V152) PE

sc-53236 PE
200 µg/ml
$349.00

Can cyclin B1 (V152): sc-53236 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin B1 (V152): sc-53236 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by 33wen from Perfect experimentThis antibody is great, specific, clean and strong results by WB
Date published: 2017-08-25
Rated 5 out of 5 by 134bei from Good antibody for IFGood antibody for IF staining in human colon tissue
Date published: 2017-04-18
Rated 4 out of 5 by QZ from Reasonably specific and sensitiveReasonably specific and sensitive, good results in WB with HeLa, PMA treated HeLa, K-562, PMA treated K-562, Jurkat and PMA treated Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by TS from Good antibody for WesternGood antibody for Western, publication with epithelial ovarian cancer cell lines OVCA420, OVCA432, OVCA433, SKOV3, and HEY. -SCBT Publication Review
Date published: 2014-12-14
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CCNB1/cyclin B1 抗体 (V152) is rated 4.5 out of 5 by 4.
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