Date published: 2025-9-7
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CUL-4 Antibody (H-11) - Western Blotting - Image 356696
CUL-4 抗体 (H-11): sc-377188. PC-12 (A), HeLa (B), NIH/3T3 (C), HL-60 (D) 和 Jurkat (E) 全细胞裂解液中 CUL-4 表达的 Western 印迹分析.
CUL-4 抗体 (H-11): sc-377188. PC-12 (A), HeLa (B), NIH/3T3 (C), HL-60 (D) 和 Jurkat (E) 全细胞裂解液中 CUL-4 表达的 Western 印迹分析.

CUL-4 抗体 (H-11): sc-377188

4.6(5)
寫評論提問

说明书
  • CUL-4 抗体 H-11 是小鼠单克隆 IgG1 κ，CUL-4抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列536-597位于human物种的CUL-4 (cullin-4)的C-terminus附近
  • CUL-4 抗体 (H-11) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的CUL-4A and CUL-4B; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗CUL-4抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CUL-4 (H-11): sc-377188的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CUL-4 Antibody (H-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CUL-4 Antibody (H-11) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

附加CUL抗体，包括CUL-1, CUL-2, CUL-3, CUL-5, CUL-7和CUL-4

CUL-4抗体（H-11）是一种IgG1 κ小鼠单克隆CUL-4抗体（也称为Cullin 4抗体或CUL4抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CUL-4蛋白。CUL-4抗体（H-11）既有非偶联型抗CUL-4抗体形式，也有多种偶联型抗CUL-4抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Cullin蛋白构成了一个独特的介导物家族，参与泛素（Ub）介导的蛋白质水解的选择性靶向。Cul-1是酵母中Cdc53的哺乳动物同源物，是指定为SCF的E3泛素连接酶复合物的组成部分。SCF（Skp1/Cul-1/F-box蛋白复合物）由Skp1与Cul-1和F-box蛋白（如Skp2）结合而成，后者决定了复合物的底物特异性。Cul-1介导的泛素化导致细胞周期蛋白cyclin D、p21和cyclin E的降解。另一种cullin，CUL-3促进cyclin E的降解，而不依赖于SCF活性，而CUL-2与肿瘤抑制蛋白VHL和elongin B结合形成VBC复合物，其结构类似于SCF连接酶。CUL-4与类泛素蛋白Nedd-8结合时，也会发生蛋白质水解，其中CUL-4也作为多功能E3复合物的活性成分发挥作用。CUL-5，或血管升压素激活的钙动员蛋白（VACM-1）也包含在cullin家族中，因为它与CUL-1具有大量的序列同源性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CUL-4 抗体 (H-11) 参考文献:

  1. 一环定江山的 E3 泛素连接酶超家族。  |  Tyers, M. and Willems, AR. 1999. Science. 284: 601, 603-4. PMID: 10328744
  2. Cullin-3靶向泛素化细胞周期蛋白E，控制哺乳动物细胞的S期。  |  Singer, JD., et al. 1999. Genes Dev. 13: 2375-87. PMID: 10500095
  3. 鉴定作为活性 E3 泛素连接酶复合物一部分的 von Hippel-lindau 肿瘤抑制蛋白。  |  Iwai, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 12436-41. PMID: 10535940
  4. CUL-4 泛素连接酶通过限制 DNA 复制许可来维持基因组的稳定性。  |  Zhong, W., et al. 2003. Nature. 423: 885-9. PMID: 12815436
  5. 草履虫的复制许可因子 CDT-1 是 CUL-4/DDB-1 复合物降解的靶标。  |  Kim, Y. and Kipreos, ET. 2007. Mol Cell Biol. 27: 1394-406. PMID: 17145765
  6. 秀丽隐杆线虫CUL-4基因的人类同源基因的过度表达与淋巴结阴性乳腺癌的不良预后有关。  |  Schindl, M., et al. 2007. Anticancer Res. 27: 949-52. PMID: 17465225
  7. 秀丽隐杆线虫CUL-4蛋白过表达的人类同源物与上皮性卵巢肿瘤的恶性潜能和癌症的不良预后有关。  |  Birner, P., et al. 2012. J Clin Pathol. 65: 507-11. PMID: 22447918
  8. CUL-4抑制可恢复遗传性痉挛性截瘫模型中的痉挛蛋白水平并减少缺陷。  |  Sardina, F., et al. 2024. Brain. 147: 3534-3546. PMID: 38551087
  9. cul-1是秀丽隐杆线虫细胞周期退出的必要条件，并确定了一个新的基因家族。  |  Kipreos, ET., et al. 1996. Cell. 85: 829-39. PMID: 8681378
  10. 位于染色体 11q22-23 上的 Cullin 基因家族成员人类 VACM-1 的鉴定和表达分析。  |  Byrd, PJ., et al. 1997. Genome Res. 7: 71-5. PMID: 9037604
  11. 在原发性乳腺癌中，草履虫 cul-4 基因的人类同源物被扩增和过表达。  |  Chen, LC., et al. 1998. Cancer Res. 58: 3677-83. PMID: 9721878
  12. 人类 CUL-1 与 SKP1/SKP2 复合物结合，并调节 p21（CIP1/WAF1）和细胞周期蛋白 D 蛋白。  |  Yu, ZK., et al. 1998. Proc Natl Acad Sci U S A. 95: 11324-9. PMID: 9736735

订购信息

产品名称产品编号规格价格数量收藏夹

CUL-4 抗体 (H-11)

sc-377188
200 µg/ml
$316.00

CUL-4 (H-11): m-IgG Fc BP-HRP 套装

sc-529987
200 µg Ab; 10 µg BP
$354.00

CUL-4 (H-11): m-IgGκ BP-HRP 套装

sc-523227
200 µg Ab, 40 µg BP
$354.00

CUL-4 (H-11): m-IgG1 BP-HRP 套装

sc-543804
200 µg Ab; 20 µg BP
$354.00

CUL-4 抗体 (H-11) AC

sc-377188 AC
500 µg/ml, 25% agarose
$416.00

CUL-4 抗体 (H-11) HRP

sc-377188 HRP
200 µg/ml
$316.00

CUL-4 抗体 (H-11) FITC

sc-377188 FITC
200 µg/ml
$330.00

CUL-4 抗体 (H-11) PE

sc-377188 PE
200 µg/ml
$343.00

CUL-4 抗体 (H-11) Alexa Fluor® 488

sc-377188 AF488
200 µg/ml
$357.00

CUL-4 抗体 (H-11) Alexa Fluor® 546

sc-377188 AF546
200 µg/ml
$357.00

CUL-4 抗体 (H-11) Alexa Fluor® 594

sc-377188 AF594
200 µg/ml
$357.00

CUL-4 抗体 (H-11) Alexa Fluor® 647

sc-377188 AF647
200 µg/ml
$357.00

CUL-4 抗体 (H-11) Alexa Fluor® 680

sc-377188 AF680
200 µg/ml
$357.00

CUL-4 抗体 (H-11) Alexa Fluor® 790

sc-377188 AF790
200 µg/ml
$357.00

does it work well by IHC?

Asked by: czczcz
Thank you for your question. Anti-CUL-4 Antibody (H-11): sc-377188 is recommended for detection of CUL-4A and CUL-4B of mouse, rat, human and origin by WB, IP, IF and ELISA. We do not recommend it for IHC applications.
Answered by: Tech Support Europe
Date published: 2022-02-17

Can you distinguish between CUL4A and CUL4B with this antibody? The old C-19 was able to. Thank you

Asked by: aliceusa
Thank you for your question. This antibody was raised against amino acids 536-597 mapping near the C-terminus of CUL-4 (cullin-4) of human origin. It can detect both CUL-4A and CUL-4B and I would not expect it to distinguish between the two.
Answered by: Tech Service
Date published: 2020-06-02

What is the recommended starting dilution for Western blot for sc-377188: CUL-4 (H-11) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from very nice wbNice Western Blot data of CUL4 expression in HeLa whole cell lysate (25 μg protein lysate; ab dilution 1:500; incubation at 4°C overnight)
Date published: 2021-07-23
Rated 4 out of 5 by from Fantastic Antibody!!The antibody works well at 1:500 dilution on Western Blot.
Date published: 2018-01-18
Rated 5 out of 5 by from excellent thingA free produt for me before 20 days, western blot was performed by using human kidney cancer cell, and it worked very well.
Date published: 2017-07-11
Rated 5 out of 5 by from a very good antibodyThis antibody works well for WB at 1:500 dilution. I gave it five -star rating because it outperforms another antibody I purchased from a different source. .
Date published: 2017-07-05
Rated 5 out of 5 by from Produced positive Western blot data of CULProduced positive Western blot data of CUL-4 expression in SK-MEL-28 and A-375 whole cell lysates. -SCBT QC
Date published: 2014-08-09
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CUL-4 抗体 (H-11) is rated 4.6 out of 5 by 5.
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