Date published: 2025-9-7
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CTGF Antibody (E-5) - Western Blotting - Image 372613
CTGF 抗体 (E-5): sc-365970. HeLa (A) 和 A549 (B) 全细胞裂解液中 CTGF 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.
CTGF 抗体 (E-5): sc-365970. HeLa (A) 和 A549 (B) 全细胞裂解液中 CTGF 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.

CTGF 抗体 (E-5): sc-365970

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寫評論提問

说明书
  • CTGF 抗体 E-5 是小鼠单克隆 IgG2a κ，CTGF抗体, 在82篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CTGF的内部区域内的氨基酸170-207之间
  • CTGF 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CTGF; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗CTGF抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CTGF (E-5): sc-365970的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是CTGF Antibody (E-5) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与CTGF Antibody (E-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CTGF抗体（E-5）是一种IgG2a κ小鼠单克隆CTGF抗体（也称为CCN2抗体或IGFBP-8抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的CTGF蛋白。CTGF抗体（E-5）既可以是非偶联的抗CTGF抗体形式，也可以是多种偶联形式的抗CTGF抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。结缔组织生长因子（CTGF，也称为肥大性软骨细胞特异性基因产物24或Hcs24）是CCN家族即时早期蛋白的成员之一，参与细胞增殖、迁移和基质生成。CTGF是一种富含半胱氨酸的肽，由内皮细胞、成纤维细胞、平滑肌细胞和肌成纤维细胞分泌。其在多种人和动物纤维化疾病中的表达增加。具体而言，已观察到CTGF在人增生性和纤维生成性肾脏疾病中表达强烈上调。此外，CTGF是血管平滑肌细胞（VSMC）的生长因子，可能在体外促进VSMC的生长和迁移中发挥类似作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CTGF 抗体 (E-5) 参考文献:

  1. 结缔组织生长因子（CTGF）可刺激体外血管平滑肌细胞的生长和迁移。  |  Fan, WH., et al. 2000. Eur J Cell Biol. 79: 915-23. PMID: 11152282
  2. 实验性增生性肾小球肾炎期间结缔组织生长因子的表达动力学。  |  Ito, Y., et al. 2001. J Am Soc Nephrol. 12: 472-484. PMID: 11181795
  3. 小儿肌纤维母细胞瘤中结缔组织生长因子的表达。  |  Kasaragod, AB., et al. 2001. Pediatr Dev Pathol. 4: 37-45. PMID: 11200489
  4. 结缔组织生长因子/肥大软骨细胞特异性基因产物24的过表达会降低成年小鼠的骨密度并诱发侏儒症。  |  Nakanishi, T., et al. 2001. Biochem Biophys Res Commun. 281: 678-81. PMID: 11237711
  5. 大鼠垂体前叶骨质素和结缔组织生长因子转录本的表达与调控  |  Ehrchen, J., et al. 2001. J Endocrinol. 169: 87-96. PMID: 11250650
  6. Nov, CYR61 和 CTGF 基因在人类肝细胞癌中的表达。  |  Hirasaki, S., et al. 2001. Hepatol Res. 19: 294-305. PMID: 11251312

订购信息

产品名称产品编号规格价格数量收藏夹

CTGF 抗体 (E-5)

sc-365970
200 µg/ml
$316.00

CTGF (E-5): m-IgG Fc BP-HRP 套装

sc-529530
200 µg Ab; 10 µg BP
$354.00

CTGF (E-5): m-IgGκ BP-HRP 套装

sc-522447
200 µg Ab, 40 µg BP
$354.00

CTGF 抗体 (E-5) AC

sc-365970 AC
500 µg/ml, 25% agarose
$416.00

CTGF 抗体 (E-5) HRP

sc-365970 HRP
200 µg/ml
$316.00

CTGF 抗体 (E-5) FITC

sc-365970 FITC
200 µg/ml
$330.00

CTGF 抗体 (E-5) PE

sc-365970 PE
200 µg/ml
$343.00

CTGF 抗体 (E-5) Alexa Fluor® 488

sc-365970 AF488
200 µg/ml
$357.00

CTGF 抗体 (E-5) Alexa Fluor® 546

sc-365970 AF546
200 µg/ml
$357.00

CTGF 抗体 (E-5) Alexa Fluor® 594

sc-365970 AF594
200 µg/ml
$357.00

CTGF 抗体 (E-5) Alexa Fluor® 647

sc-365970 AF647
200 µg/ml
$357.00

CTGF 抗体 (E-5) Alexa Fluor® 680

sc-365970 AF680
200 µg/ml
$357.00

CTGF 抗体 (E-5) Alexa Fluor® 790

sc-365970 AF790
200 µg/ml
$357.00

CTGF (E-5) 中和勝肽

sc-365970 P
100 µg/0.5 ml
$68.00

What is the formulation buffer of the antibody? Does it contain sodium azide?

Asked by: vanessaw
Thank you for your question. CTGF Antibody (E-5): sc-365970 is supplied as 200 µg antibody in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2021-03-22

Hi I'm using your antibody - is it meant to be nuclear or cytoplasmic or nuclear-cytoplasmic in staining? and secondly does it stain neuronal tissue/cells?

Asked by: stephp
Thank you for your question. CTGF is detectable in the extracellular matrix. CTGF Antibody (E-5): sc-365970 is recommended for detection of CTGF of mouse, rat and human origin by WB, IP, IF and ELISA in any tissue that expresses the protein. It is also reactive with additional species, including and equine, canine and bovine. There are currently no publications showing staining of neuronal tissues or cells that we are aware of. Please contact our technical service for further information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Why double band is detected (about 38 and 42 kDa) using anti-ctgf?

Asked by: Diogo
Thank you for your question. We have not observed a double band when using this mouse monoclonal antibody, CTGF (E-5): sc-365970, for Western blotting. There is representative Western blot data available on our website. The predicted molecular weight for CTGF is 38 kDa. Please contact our Technical Service Department if you would like help troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-12

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 5 out of 5 by from CTGFデータシートに記載されてアプリケーションで問題なく、CTGF蛋白質のバンドが検出できました。良い抗体です。
Date published: 2025-04-19
Rated 5 out of 5 by from Goof for western blotting.I used this CTGF antibody using mouse lung tissue and worked well!!
Date published: 2024-09-29
Rated 5 out of 5 by from Great for Western blotNice prominent band for vascular smooth muscle cells!
Date published: 2019-02-19
Rated 4 out of 5 by from Tested positiveWe tested this on lysates from several cell lines together with anti-CTGF antibody and some of them worked positively as expected.
Date published: 2018-06-03
Rated 5 out of 5 by from Great stain, specificI stained paraffin samples at 1:100 and got great results.
Date published: 2018-03-08
Rated 3 out of 5 by from A good antibodyI applied a sample of CTGF, I think it's OK. The antibody concentration is 1:500. The only problem is that it locates at exactly the same position with GAPDH. And by far all the bands I got is of the same trend with GAPDH(I use tubulin instead for CTGF). So I'm not sure whether it is CTGF bands or just non-specific bands on GAPDH location.
Date published: 2018-02-26
Rated 1 out of 5 by from Kind of terribleUsed this antibody at 1:1000 dilution on a western blot with 100 ug of protein from tissue. Really could not see any band at all.
Date published: 2018-02-21
Rated 4 out of 5 by from good signalThe antibody was tested in western blot on rat tissue samples and gave a good signal. However, double bands are detected.
Date published: 2017-12-13
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CTGF 抗体 (E-5) is rated 4.4 out of 5 by 31.
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