Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Csk Antibody (E-3) - Western Blotting - Image 296247
Csk 抗体 (E-3): sc-166560. Jurkat (A), AML-193 (B), EOC 20 (C), BC3H1 (D) 和 CTLL-2 (E) 全细胞裂解液中 Csk 表达的 Western 印迹分析.
Csk 抗体 (E-3): sc-166560. Jurkat (A), AML-193 (B), EOC 20 (C), BC3H1 (D) 和 CTLL-2 (E) 全细胞裂解液中 Csk 表达的 Western 印迹分析.

Csk 抗体 (E-3): sc-166560

4.7(7)
寫評論提問

说明书
  • Csk 抗体 E-3 是小鼠单克隆 IgG1 κ，Csk抗体, 在6篇文献中引用，规格为200 µg/ml
  • 与氨基酸序列C-terminus（h）对应的多肽序列（Csk的human起源）
  • Csk 抗体 (E-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Csk p50; 也和以下物种反应，包括： 和 canine and porcine
  • 抗Csk抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Csk (E-3): sc-166560的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Csk 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Csk 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Csk抗体（E-3）是一种IgG1 κ小鼠单克隆Csk抗体（也称为CSK非受体酪氨酸激酶抗体、酪氨酸蛋白激酶CSK抗体、蛋白质酪氨酸激酶CYL抗体、C-Src酪氨酸激酶抗体或C-Src激酶抗体），该抗体可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人的Csk蛋白。Csk抗体（E-3）既可以是非偶联的抗Csk抗体形式，也可以是多种偶联形式的抗Csk抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Src基因家族的所有酪氨酸激酶成员都以羧基末端结构域的酪氨酸为特征，该酪氨酸在酶的非活性形式中高度磷酸化，在酶活性时磷酸化程度则低得多。对于Src p60，Y527就是这种酪氨酸；然而，当Y527被苯丙氨酸取代时，c-Src的突变体发生转化，并显示出比其正常对应物高5-10倍的激酶活性。Csk已被鉴定为Src相关酪氨酸激酶，具有SH2和SH3结构域以及催化结构域，但缺少SH3结构域氨基末端以及羧基末端调节序列的序列。Csk在Y527上磷酸化Src，并通过羧基末端调节位点的酪氨酸磷酸化下调Lyn、Fyn和Lck。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Csk 抗体 (E-3) 参考文献:

  1. 克隆出一种蛋白酪氨酸激酶的互补 DNA，这种激酶能特异性地使 p60c-src 的一个负调控位点磷酸化。  |  Nada, S., et al. 1991. Nature. 351: 69-72. PMID: 1709258
  2. 一种参与调节 pp60c-src 功能的蛋白酪氨酸激酶。  |  Okada, M. and Nakagawa, H. 1989. J Biol Chem. 264: 20886-93. PMID: 2480346
  3. Csk 在肠上皮屏障功能和预防结肠炎中的作用。  |  Sun, C., et al. 2018. Biochem Biophys Res Commun. 504: 109-114. PMID: 30173891
  4. Crk 介导 Csk-Hippo 信号传导，独立于 Yap 酪氨酸磷酸化，诱导细胞挤出。  |  Hannan, A., et al. 2024. bioRxiv.. PMID: 39005335
  5. 人类 CSK 基因座的特征。  |  Bräuninger, A., et al. 1993. Oncogene. 8: 1365-9. PMID: 7683131
  6. Src 调节的时间和方式。  |  Cooper, JA. and Howell, B. 1993. Cell. 73: 1051-4. PMID: 7685656
  7. csk 基因编码 Src 家族酪氨酸激酶的负调控因子，该基因的缺失会导致小鼠神经管缺陷和胚胎死亡。  |  Imamoto, A. and Soriano, P. 1993. Cell. 73: 1117-24. PMID: 7685657
  8. Csk 对 c-Src 活性的抑制需要 Src 的 SH2 和 SH3 结构域。  |  Superti-Furga, G., et al. 1993. EMBO J. 12: 2625-34. PMID: 7687537
  9. 酪氨酸蛋白激酶 p50csk 对 T 细胞受体信号的负调控。  |  Chow, LM., et al. 1993. Nature. 365: 156-60. PMID: 8371758
  10. 缺乏 Csk 的小鼠胚胎中 Src 家族激酶的持续激活。  |  Nada, S., et al. 1993. Cell. 73: 1125-35. PMID: 8513497

订购信息

产品名称产品编号规格价格数量收藏夹

Csk 抗体 (E-3)

sc-166560
200 µg/ml
$316.00

Csk (E-3): m-IgG Fc BP-HRP 套装

sc-528994
200 µg Ab; 10 µg BP
$354.00

Csk (E-3): m-IgGκ BP-HRP 套装

sc-521656
200 µg Ab, 40 µg BP
$354.00

Csk (E-3): m-IgG1 BP-HRP 套装

sc-543255
200 µg Ab; 20 µg BP
$354.00

Csk 抗体 (E-3) AC

sc-166560 AC
500 µg/ml, 25% agarose
$416.00

Csk 抗体 (E-3) HRP

sc-166560 HRP
200 µg/ml
$316.00

Csk 抗体 (E-3) FITC

sc-166560 FITC
200 µg/ml
$330.00

Csk 抗体 (E-3) PE

sc-166560 PE
200 µg/ml
$343.00

Csk 抗体 (E-3) Alexa Fluor® 488

sc-166560 AF488
200 µg/ml
$357.00

Csk 抗体 (E-3) Alexa Fluor® 546

sc-166560 AF546
200 µg/ml
$357.00

Csk 抗体 (E-3) Alexa Fluor® 594

sc-166560 AF594
200 µg/ml
$357.00

Csk 抗体 (E-3) Alexa Fluor® 647

sc-166560 AF647
200 µg/ml
$357.00

Csk 抗体 (E-3) Alexa Fluor® 680

sc-166560 AF680
200 µg/ml
$357.00

Csk 抗体 (E-3) Alexa Fluor® 790

sc-166560 AF790
200 µg/ml
$357.00

Csk (E-3) 中和勝肽

sc-166560 P
100 µg/0.5 ml
$68.00

Does the anti-Csk (E-3): sc-166560 work well for Immunoprecipitation of Csk from mouse cells? Is it as good as the rab. polycl. sc-286. How long do you incubate the lysate with the antibody, and how do you collect the complex protein-antibody: Protein G? 

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-07

What application is the blocking peptide sc-166560 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2022-01-07
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_166560, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Be sure to optimize protocols.Works well with IF and whole cell lysates for westerns, but we've been having difficulty with subcellular fractions. This may be due to different protocol conditions on our part as this has happened with other antibodies from different companies. Just need to figure out what works for this protein.
Date published: 2019-04-24
Rated 5 out of 5 by from Good!There is a good signal when it was used for Western blot
Date published: 2018-11-02
Rated 5 out of 5 by from good featuresI applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 4 out of 5 by from Csk antibodyThe Csk antibody is working fine for Western blot.
Date published: 2018-01-05
Rated 5 out of 5 by from Good for IP western blotI used this antibody for my IP WB on human samples, and it gave me strong band
Date published: 2017-03-30
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using Jurkat, AML-193, EOC 20, BC3H1 and CTLL-2 whole cell lysates. -SCBT QC
Date published: 2015-06-07
Rated 5 out of 5 by from Robust band of interest observed in WB inRobust band of interest observed in WB in human and mouse Csk transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-11-06
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_166560, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
Csk 抗体 (E-3) is rated 4.7 out of 5 by 7.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_166560, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。