Date published: 2025-9-7

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CAP-18 抗体 (G-1): sc-166055

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说明书
  • CAP-18 抗体 G-1 是小鼠单克隆 IgG2a κ,CAP-18抗体, 在10篇文献中引用,规格为200 µg/ml
  • 针对氨基酸6-175,在CAP-18的rat起源的C-terminus处进行映射
  • CAP-18 抗体 (G-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse 和rat 来源的 CAP-18
  • 抗CAP-18抗体(G-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CAP-18 (G-1): sc-166055免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是CAP-18 Antibody (G-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CAP-18 Antibody (G-1) 打包提供(请参阅下面的订购信息)。

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関連項目

CAP-18抗体(G-1)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠和大鼠来源的CAP-18。CAP-18 (G-1) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CAP-18,也称为抗菌肽,在先天免疫反应中起着至关重要的作用,它作为一种抗菌剂,有助于预防感染和调节炎症。CAP-18主要存在于中性粒细胞、骨髓和睾丸中,在牛皮癣和皮炎等炎症性皮肤病中尤为重要,因为这些疾病中CAP-18的水平会升高。CAP-18含有抗菌肽LL-37,该肽能够形成两亲性α螺旋构象,使CAP-18能够与细菌脂多糖有效相互作用,并吸引免疫细胞到达感染部位。LL-37在炎症反应中的存在凸显了CAP-18在宿主防御机制中的重要性,这使得抗CAP-18抗体(G-1)成为研究人员研究抗菌肽及其在免疫反应中的作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CAP-18 抗体 (G-1) 参考文献:

  1. 人类 cathelicidin(hCAP-18)通过蛋白酶 3 在细胞外裂解加工成抗菌肽 LL-37。  |  Sørensen, OE., et al. 2001. Blood. 97: 3951-9. PMID: 11389039
  2. 人类抗菌肽 LL-37 中的一种猫肝菌素家族可诱导肥大细胞趋化。  |  Niyonsaba, F., et al. 2002. Immunology. 106: 20-6. PMID: 11972628
  3. 唾液腺和唾液中都表达有猫肽抗菌肽。  |  Murakami, M., et al. 2002. J Dent Res. 81: 845-50. PMID: 12454100
  4. 小鼠和人类新生儿皮肤表达的抗菌肽水平增加:适应性反应发育过程中的先天免疫。  |  Dorschner, RA., et al. 2003. Pediatr Res. 53: 566-72. PMID: 12612195
  5. 大鼠 cathelicidin rCRAMP 的系统发育, 加工和表达:先天性抗菌肽模型。  |  Termén, S., et al. 2003. Cell Mol Life Sci. 60: 536-49. PMID: 12737313
  6. cathelicidins--结构, 功能和进化。  |  Tomasinsig, L. and Zanetti, M. 2005. Curr Protein Pept Sci. 6: 23-34. PMID: 15638766
  7. 作为抗中性粒细胞胞浆抗体相关性脉管炎血清学标志物和潜在致病因素的猫嗜血杆菌素抗菌肽。  |  Gasim, A. 2014. Arthritis Res Ther. 16: 105. PMID: 25164257
  8. Cathelicidin抗菌肽在肝细胞癌中发挥抑癌作用  |  Huang, LH., et al. 2023. Int J Mol Sci. 24: PMID: 37958632
  9. 嗜中性粒细胞和神经元中Cathelicidin抗菌肽的表达可拮抗神经炎症。  |  Verma, SC., et al. 2024. J Clin Invest. 135: PMID: 39656548
  10. 一种在骨髓中表达的新型小鼠 cathelin 样蛋白。  |  Popsueva, AE., et al. 1996. FEBS Lett. 391: 5-8. PMID: 8706928
  11. 鉴定 CRAMP,一种在胚胎和成年小鼠中表达的 cathelin 相关抗菌肽。  |  Gallo, RL., et al. 1997. J Biol Chem. 272: 13088-93. PMID: 9148921
  12. 人类角质细胞在发生炎症时会诱导抗菌肽 LL-37 的编码基因表达。  |  Frohm, M., et al. 1997. J Biol Chem. 272: 15258-63. PMID: 9182550

订购信息

产品名称产品编号规格价格数量收藏夹

CAP-18 抗体 (G-1)

sc-166055
200 µg/ml
$316.00

CAP-18 (G-1): m-IgG Fc BP-HRP 套装

sc-528899
200 µg Ab; 10 µg BP
$354.00

CAP-18 (G-1): m-IgGκ BP-HRP 套装

sc-521497
200 µg Ab, 40 µg BP
$354.00

CAP-18 (G-1): m-IgG2a BP-HRP 套装

sc-547201
200 µg Ab; 10 µg BP
$354.00

CAP-18 抗体 (G-1) AC

sc-166055 AC
500 µg/ml, 25% agarose
$416.00

CAP-18 抗体 (G-1) HRP

sc-166055 HRP
200 µg/ml
$316.00

CAP-18 抗体 (G-1) FITC

sc-166055 FITC
200 µg/ml
$330.00

CAP-18 抗体 (G-1) PE

sc-166055 PE
200 µg/ml
$343.00

CAP-18 抗体 (G-1) Alexa Fluor® 488

sc-166055 AF488
200 µg/ml
$357.00

CAP-18 抗体 (G-1) Alexa Fluor® 546

sc-166055 AF546
200 µg/ml
$357.00

CAP-18 抗体 (G-1) Alexa Fluor® 594

sc-166055 AF594
200 µg/ml
$357.00

CAP-18 抗体 (G-1) Alexa Fluor® 647

sc-166055 AF647
200 µg/ml
$357.00

CAP-18 抗体 (G-1) Alexa Fluor® 680

sc-166055 AF680
200 µg/ml
$357.00

CAP-18 抗体 (G-1) Alexa Fluor® 790

sc-166055 AF790
200 µg/ml
$357.00

Is there an expected non specific band between 60-70 kDa? I have a very strong band in the area with faint or no bands near the 20-30 kDa range

Asked by: pciari
Thank you for your question. We do not expect a nonspecific band. Please contact our Technical Support department to troubleshoot the antibody. Please call (800)-457-3801 or email scbt@scbt.com
Answered by: BlakeJ
Date published: 2022-09-07

We intend to detect mCRAMP in mouse kidney tissue by western blot using this antibody. However, in the reference you suggested, CRAMP band is observed at 20KD for PMID#33468624 and #30094093, and at 28kDa for #32682918. What band is desirable to read when

Asked by: hongsang38
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hello. I would like to ask whether the CRAMP Antibody (G-1): sc-166055 recognizes C-terminal antimicrobial peptide released from precursor protein (ISRLAGLLRKGGEKIGEKLKKIGQKIKNFFQKLVPQPE). Thank you! Piotr

Asked by: pkol
Thank you for your question. CRAMP Antibody (G-1): sc-166055 has been raised against amino acids 6-175 mapping at the C-terminus of CRAMP of rat origin. Since this amino acid range corresponds to a longer sequence than the one you have provided, it is possible that the antibody will not bind your sequence. This is a monoclonal antibody, so it only has one distinct epitope within its epitope region. Since no epitope mapping has been performed as of yet, we cannot say whether or not the antibody would recognize the sequence you reference.
Answered by: Tech Support Europe
Date published: 2020-08-28

Can CRAMP (G-1): sc-166055 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CRAMP (G-1): sc-166055 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very clearWonderful results with CRAMP Antibody (G-1) monoclonal antibody, this is good
Date published: 2017-05-19
Rated 5 out of 5 by from strong positive bandStrong positive band observed with CRAMP Antibody (G-1) with little to no background
Date published: 2017-01-31
Rated 5 out of 5 by from Produced nice Western blot data of CRAMPProduced nice Western blot data of CRAMP expression in CSMLO whole cell lysate. -SCBT QC
Date published: 2014-09-10
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CAP-18 抗体 (G-1) is rated 5.0 out of 5 by 3.
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