Date published: 2025-9-7

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connexin 43 抗体 (F-7): sc-271837

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说明书
  • connexin 43 抗体 F-7 是小鼠单克隆 IgG2a κ,connexin 43抗体, 在57篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的connexin 43的C-terminus的氨基酸357-381之间
  • connexin 43 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 connexin 43; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗connexin 43抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取connexin 43 (F-7): sc-271837免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRPm-IgGκ BP-HRP 是 connexin 43 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与connexin 43 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

connexin 43抗体(F-7)是一种IgG2a κ小鼠单克隆connexin 43抗体(也称为GJA1抗体、间隙连接蛋白α1抗体或CX43抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的connexin 43蛋白。connexin 43抗体(F-7)既可作为非偶联抗connexin 43抗体,也可作为多种偶联形式的抗connexin 43抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。连接蛋白是一组形成六聚体以构成连接子的间隙连接蛋白。连接子簇形成间隙连接,通过该连接,低分子量蛋白质可以从细胞扩散到细胞。具有恶性表型的几种哺乳动物细胞表现出连接蛋白表达和间隙连接通讯的减少。在Src转化的细胞中,间隙连接通讯减少,这似乎与connexin 43的酪氨酸磷酸化有关。活化的c-Src在Tyr 265上磷酸化connexin 43的C端尾部,导致两种蛋白之间的稳定相互作用,从而导致间隙连接通讯的抑制。除了酪氨酸磷酸化外,还已显示在没有Src激酶的情况下,connexin 43在丝氨酸上被磷酸化,并且在表达Src激酶的细胞(如c-Src和/或pp60v-Src)中,connexin 43在丝氨酸和酪氨酸上都被磷酸化。在人类血管内皮细胞中,connexin 43在有丝分裂期间发生翻译后修饰。connexin 43的有丝分裂特异性磷酸化与间隙连接细胞间通讯的短暂丧失和connexin 43的重新分布相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

connexin 43 抗体 (F-7)

sc-271837
200 µg/ml
$316.00

connexin 43 (F-7): m-IgGκ BP-HRP 套装

sc-522076
200 µg Ab, 40 µg BP
$354.00

connexin 43 (F-7): m-IgG2a BP-HRP 套装

sc-546108
200 µg Ab; 10 µg BP
$354.00

connexin 43 抗体 (F-7) AC

sc-271837 AC
500 µg/ml, 25% agarose
$416.00

connexin 43 抗体 (F-7) HRP

sc-271837 HRP
200 µg/ml
$316.00

connexin 43 抗体 (F-7) FITC

sc-271837 FITC
200 µg/ml
$330.00

connexin 43 抗体 (F-7) PE

sc-271837 PE
200 µg/ml
$343.00

connexin 43 抗体 (F-7) Alexa Fluor® 488

sc-271837 AF488
200 µg/ml
$357.00

connexin 43 抗体 (F-7) Alexa Fluor® 546

sc-271837 AF546
200 µg/ml
$357.00

connexin 43 抗体 (F-7) Alexa Fluor® 594

sc-271837 AF594
200 µg/ml
$357.00

connexin 43 抗体 (F-7) Alexa Fluor® 647

sc-271837 AF647
200 µg/ml
$357.00

connexin 43 抗体 (F-7) Alexa Fluor® 680

sc-271837 AF680
200 µg/ml
$357.00

connexin 43 抗体 (F-7) Alexa Fluor® 790

sc-271837 AF790
200 µg/ml
$357.00

connexin 43 (F-7) 中和勝肽

sc-271837 P
100 µg/0.5 ml
$68.00

Hello, I am a researcher in turkey in selcuk un. in konya city. I have bought a antibody (sc-271837, lot: ı1417). I will use this antibody for western blot. in the which solution and concentrations should ı dilüate this antibody? Pleace fast answer me

Asked by: kemal
Thank you for your question. We recommend blocking and incubating both primary and secondary antibody in 5% nonfat milk + TBST, and the recommended starting dilution is 1:200 for Western blotting. A link to our complete recommended protocol for Western blotting is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2019-01-14

What is host of this antibody (connexin 43 Antibody (F-7): sc-271837)

Asked by: kemal
Thank you for your question. connexin 43 Antibody (F-7): sc-271837 is a mouse monoclonal IgG2a antibody.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

is there an SDS for this product

Asked by: TechRC
Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support 10
Date published: 2017-09-21

I am hoping to use this connexin 43 (F-7): sc-271837 antibody to stain paraffin embedded tissue sections. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
This connexin 43 antibody has been validated for use in IHC with paraffin-embedded sections and we defintely recommend performing antigen retrieval. Best results are seen using with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2024-08-22
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Rated 5 out of 5 by from Good antibody1:200 diluted for wb of rat heart protein,the band is strong and special.
Date published: 2020-02-02
Rated 5 out of 5 by from Works for mouse testis IHCWe got a sample of this antibody and it worked nicely for IHC on testis sections fixed in Bouins (1:400 dilution).
Date published: 2019-09-06
Rated 5 out of 5 by from With nice single bandI bought this antibody two weeks ago and it works in my rat kidney cells.
Date published: 2019-01-30
Rated 5 out of 5 by from Great for IFWorks very well with rat tissue cryosections fixed in ice-cold methanol; little or no background (dilution tested = 1:100).
Date published: 2018-02-20
Rated 1 out of 5 by from Immunohistochemistry frozen & free floatingI tested the antibody for an immunohistochemistry on mouse embryo E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/50, Permeabilization solution: PBS 1X- Triton 0,2%(& 0,4%) - Goat serum 10%. It did not work.
Date published: 2017-10-27
Rated 3 out of 5 by from It works on human and pig tissues for immunofluoreI did test this Ab on human SFBLs and pig tissues and it works well.
Date published: 2017-10-26
Rated 5 out of 5 by from good for wbI dilute the antibody at 1:1000 and incubate at 4℃ overnight.
Date published: 2017-09-18
Rated 5 out of 5 by from Work Well on WBWe used this antibody for WB.A clear brand can be found
Date published: 2017-08-03
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connexin 43 抗体 (F-7) is rated 4.4 out of 5 by 17.
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