Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CLIC1 Double Nickase Plasmid (h): sc-402391-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLIC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLIC1 Double Nickase Plasmid (h) and CLIC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLIC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLIC1 Antibody (F-9): sc-374202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIC1 Double Nickase Plasmid (h) | sc-402391-NIC | 20 µg | $410.00 | |||
CLIC1 Double Nickase Plasmid (h2) | sc-402391-NIC-2 | 20 µg | $410.00 |
CLIC1 encodes chloride intracellular channel 1, a metamorphic protein that can exist as a soluble cytosolic factor or associate with cellular membranes to influence chloride conductance, redox balance, and intracellular pH regulation. It has been linked to regulation of cell volume, cytoskeletal dynamics, and vesicular trafficking, and is frequently studied in the context of oxidative stress responses and inflammatory signaling. CLIC1 activity and expression have been reported to change during immune cell activation and cellular transformation, supporting investigation of its contributions to proliferation, migration, and survival programs. Dysregulated CLIC1 has been associated with multiple pathological contexts, including cancer biology and neuroinflammation, making it a useful target for dissecting stress-adaptive and ion homeostasis pathways.
CLIC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLIC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLIC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLIC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLIC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.