Date published: 2025-9-7
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choactase Antibody (E-7) - Western Blotting - Image 312945
雏菊酶抗体 (E-7): sc-55557. Western 印迹分析 SK-N-SH (A), HeLa (B), IMR-32 (C), Jurkat (D), Ramos (E) 和 HEK293 (F) 全细胞裂解液中 choactase 的表达.
雏菊酶抗体 (E-7): sc-55557. Western 印迹分析 SK-N-SH (A), HeLa (B), IMR-32 (C), Jurkat (D), Ramos (E) 和 HEK293 (F) 全细胞裂解液中 choactase 的表达.

choactase 抗体 (E-7): sc-55557

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说明书
  • choactase 抗体 E-7 是小鼠单克隆 IgG1 κ，choactase抗体, 在20篇文献中引用，规格为200 µg/ml
  • 抗-choactase 抗体 (E-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 all isoforms of choactase
  • choactase抗体（E-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取choactase (E-7): sc-55557的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 choactase 抗体 (E-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与choactase 抗体 (E-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

酪氨酸酶抗体（E-7）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的酪氨酸酶蛋白。抗乳酸内酯酶抗体 (E-7) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。胆碱乙酰转移酶又称胆碱酯酶，在胆碱能神经元合成乙酰胆碱的过程中发挥着关键作用，而乙酰胆碱是神经传递和认知功能所必需的。酪氨酸酶（E-7）抗体的靶标蛋白主要位于细胞质中，但长异构体也存在于细胞核中，这表明其功能作用因定位而异。多种雏菊糖苷酶 mRNA（如 R-、N1、N2-、S- 和 M-型）的存在可产生不同的酶异构体，从而影响乙酰胆碱合成的调节。酪氨酸酶的表达与神经退行性疾病密切相关，因为 NFkB 通路水平的降低和活性的增加与衰老和阿尔茨海默病有关。这表明，调节choactase的表达对维持胆碱能功能至关重要，而了解细胞定位和同工酶的分布可为认知功能衰退的治疗策略提供启示。丝氨酸 440 处的磷酸化已被确定为一种关键的翻译后修饰，可增强choactase 的催化活性，这进一步强调了它在神经元健康和信号传导中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

choactase 抗体 (E-7) 参考文献:

  1. 人胆碱乙酰转移酶 82-kDa 形式的核定位。  |  Resendes, MC., et al. 1999. J Biol Chem. 274: 19417-21. PMID: 10383456
  2. 重组人胆碱乙酰转移酶的表达, 纯化和表征：酶的磷酸化调节催化活性。  |  Dobransky, T., et al. 2000. Biochem J. 349: 141-51. PMID: 10861222
  3. 核因子 kappaB/p49 是 PC12 细胞中神经生长因子诱导的胆碱乙酰转移酶启动子活性的负调控因子。  |  Toliver-Kinsky, T., et al. 2000. J Neurochem. 75: 2241-51. PMID: 11080175
  4. 蛋白激酶 C 对 69 kDa 人胆碱乙酰转移酶丝氨酸 440 磷酸化的功能表征。  |  Dobransky, T., et al. 2001. J Biol Chem. 276: 22244-50. PMID: 11303024
  5. 人胆碱乙酰转移酶的互补 DNA 可在培养细胞中诱导出两种分子量不同的酶。  |  Oda, Y., et al. 1992. Brain Res Mol Brain Res. 16: 287-94. PMID: 1337937
  6. 阿尔茨海默病中梅纳核基底核中胆碱乙酰转移酶mRNA表达的降低：原位杂交研究。  |  Strada, O., et al. 1992. Proc Natl Acad Sci U S A. 89: 9549-53. PMID: 1409664
  7. 使用静电纺丝聚-L-乳酸/明胶支架将神经干细胞分化成运动神经元。  |  Binan, L., et al. 2014. Biomaterials. 35: 664-74. PMID: 24161168
  8. 免疫组织化学显示豚鼠自主神经节中胆碱乙酰转移酶免疫反应性前神经纤维。  |  Lindh, B., et al. 1986. Proc Natl Acad Sci U S A. 83: 5316-20. PMID: 3460095
  9. 链脲佐菌素诱导的啮齿动物 1 型和 2 型糖尿病：研究糖尿病心脏自主神经病变的模型。  |  Akinlade, OM., et al. 2021. Afr Health Sci. 21: 719-727. PMID: 34795728
  10. 猪胆碱乙酰转移酶的cDNA克隆和完整序列：体外翻译相应的RNA可产生活性蛋白。  |  Berrard, S., et al. 1987. Proc Natl Acad Sci U S A. 84: 9280-4. PMID: 3480542
  11. 运动神经元和神经肌肉接头中四种神经活性物质的合成酶：光学和电子显微镜免疫细胞化学。  |  Chan-Palay, V., et al. 1982. Proc Natl Acad Sci U S A. 79: 6717-21. PMID: 6128735
  12. 具有不同 5'-region 的人胆碱乙酰转移酶 mRNA 会产生一个 69-kDa 的主要翻译产物。  |  Misawa, H., et al. 1997. Brain Res Mol Brain Res. 44: 323-33. PMID: 9073174

订购信息

产品名称产品编号规格价格数量收藏夹

choactase 抗体 (E-7)

sc-55557
200 µg/ml
$316.00

choactase (E-7): m-IgG Fc BP-HRP 套装

sc-526863
200 µg Ab; 10 µg BP
$354.00

choactase (E-7): m-IgG1 BP-HRP 套装

sc-532236
200 µg Ab; 20 µg BP
$354.00

choactase 抗体 (E-7) AC

sc-55557 AC
500 µg/ml, 25% agarose
$416.00

choactase 抗体 (E-7) HRP

sc-55557 HRP
200 µg/ml
$316.00

choactase 抗体 (E-7) FITC

sc-55557 FITC
200 µg/ml
$330.00

choactase 抗体 (E-7) PE

sc-55557 PE
200 µg/ml
$343.00

choactase 抗体 (E-7) Alexa Fluor® 488

sc-55557 AF488
200 µg/ml
$357.00

choactase 抗体 (E-7) Alexa Fluor® 546

sc-55557 AF546
200 µg/ml
$357.00

choactase 抗体 (E-7) Alexa Fluor® 594

sc-55557 AF594
200 µg/ml
$357.00

choactase 抗体 (E-7) Alexa Fluor® 647

sc-55557 AF647
200 µg/ml
$357.00

choactase 抗体 (E-7) Alexa Fluor® 680

sc-55557 AF680
200 µg/ml
$357.00

choactase 抗体 (E-7) Alexa Fluor® 790

sc-55557 AF790
200 µg/ml
$357.00

Mr. Santa Cruz: We are working in your new monoclonal choactase antibody E-7: sc-55557 in ovarian tissue but we have found several bands that not fit to ChAT predictable molecular weight. So, we are interested to know if you have the blocking peptide.

Asked by: Jackie30
Thank you for your question. We do not offer a blocking peptide for this antibody as it was not made against a peptide. Please contact your local distributor for assistance optimizing this antibody, they will be happy to help you! You can find their contact information here: https://www.scbt.com/scbt/resources/distributors.
Answered by: Tech Service
Date published: 2018-01-17

I am using choactase (E-7): sc-55557 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from This antibody works for westernWe use this antibody for WB of mouse mammary epithelial cells. We got a good band.
Date published: 2022-06-06
Rated 3 out of 5 by from Decent data with high backgroundSome non-specific bands in WB and high background. Incubation O/N at 4 degrees diluted 1:1000 in 5% non-fat dry milk in TBS-T.
Date published: 2021-07-19
Rated 4 out of 5 by from IF works well on rat frozen liver sectionsRat liver frozen sections were subjected to immunofluorescence using choactase (E-7) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Positive signals in portal tracts were clear without any antigen retrieval.
Date published: 2018-03-14
Rated 5 out of 5 by from works for western blotWe had very good results using this antibody 1:5000 with whole cell lysates of HT-22 cells in western blot.
Date published: 2017-11-15
Rated 4 out of 5 by from it's okworks for immunofluorescence on mouse brain samples.
Date published: 2017-06-01
Rated 5 out of 5 by from works for westernWe use this often in WB the best antibodies out there, we got a good band.
Date published: 2017-05-20
Rated 5 out of 5 by from Versatile Antibody for Human SamplesThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-02-09
Rated 4 out of 5 by from Bands seen in WB using SKBands seen in WB using SK-N-SH, HeLa, Jurkat, Ramos and HEK293 whole cell lysates. -SCBT QC
Date published: 2015-07-01
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choactase 抗体 (E-7) is rated 4.2 out of 5 by 13.
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