Date published: 2025-9-6
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Cdc6 Antibody (180.2) - Western Blotting - Image 378863
Cdc6 抗体 (180. 2): sc-9964. HeLa 核提取物 (A), HeLa (B), Jurkat (C) 和 K-562 (D) 全细胞裂解液中 Cdc6 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Cdc6 抗体 (180. 2): sc-9964. HeLa 核提取物 (A), HeLa (B), Jurkat (C) 和 K-562 (D) 全细胞裂解液中 Cdc6 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Cdc6 抗体 (180.2): sc-9964

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说明书
  • Cdc6 抗体 180.2 是小鼠单克隆 IgG1 κ，Cdc6抗体, 在139篇文献中引用，规格为200 µg/ml
  • 针对全长Cdc6，human起源
  • Cdc6 抗体 (180.2) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Cdc6
  • 抗Cdc6抗体（180.2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdc6 (180.2): sc-9964的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Cdc6 抗体 (180.2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc6 抗体 (180.2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc6抗体（180.2）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光和免疫组织化学检测小鼠、大鼠和人源的Cdc6。Cdc6 (180.2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc6在DNA复制的启动中起着至关重要的作用，而DNA复制对于细胞分裂和细胞周期的正常进行至关重要。通过确保DNA复制在正确的时间发生，Cdc6有助于维持基因组的稳定性，并防止可能导致细胞功能异常或疾病的错误。Cdc6在癌症研究中尤为重要，因为Cdc6的失调会导致细胞无序增殖。Cdc6是酿酒酵母Cdc6的人类同源物，它与Cdc37等多种蛋白相互作用，有助于形成细胞周期蛋白D1/细胞周期蛋白依赖性激酶4复合物，从而凸显其在细胞周期调控中的不可或缺的作用。此外，Cdc6还与泛素连接酶（如Cdc34、Cdc27和Cdc16）相互作用，这些酶参与细胞周期蛋白的降解，进一步强调了其在细胞周期事件中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc6 抗体 (180.2) 参考文献:

  1. 嗜酸热原虫中的 Cdc6 蛋白刺激 MCM 螺旋酶的活性。  |  Haugland, GT., et al. 2006. Nucleic Acids Res. 34: 6337-44. PMID: 17108356
  2. 有丝分裂期 Cdc6 通过一种部分不依赖于 Cdc28 的机制稳定无相位促进复合体底物，这种稳定作用会因 Cdc55 的缺失而受到抑制。  |  Boronat, S. and Campbell, JL. 2007. Mol Cell Biol. 27: 1158-71. PMID: 17130241
  3. cdc25 是一种特异性酪氨酸磷酸酶，可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  4. 酵母(Saccharomyces cerevisiae)cdc突变体中人工染色体的有丝分裂传递。  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
  5. CDC6 是一种预后生物标志物，与胶质瘤的免疫浸润相关。  |  Wang, F., et al. 2022. Mol Cancer. 21: 153. PMID: 35879762
  6. GATA2-CDC6 轴调节雄激素受体阻断剂诱导的前列腺癌衰老。  |  Mourkioti, I., et al. 2023. J Exp Clin Cancer Res. 42: 187. PMID: 37507762
  7. OTUD6A对CDC6的去泛素化促进了肿瘤进展和化疗抗性  |  Cui, J., et al. 2024. Mol Cancer. 23: 86. PMID: 38685067
  8. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  9. 含有 CDC27 和 CDC16 的 20S 复合物可催化泛素与细胞周期蛋白 B 的有丝分裂特异性连接。  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
  10. 通过酵母互补克隆出 CDC34 细胞周期基因的人类同源物。  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
  11. 哺乳动物的 p50Cdc37 是 Hsp90 的蛋白激酶靶向亚基，能与 Cdk4 结合并使其稳定。  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
  12. 一种与酵母 Cdc6p 相关的人类蛋白质。  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc6 抗体 (180.2)

sc-9964
200 µg/ml
$316.00

Cdc6 (180.2): m-IgG Fc BP-HRP 套装

sc-528239
200 µg Ab; 10 µg BP
$354.00

Cdc6 (180.2): m-IgGκ BP-HRP 套装

sc-520565
200 µg Ab, 40 µg BP
$354.00

Cdc6 (180.2): m-IgG1 BP-HRP 套装

sc-542831
200 µg Ab; 20 µg BP
$354.00

Cdc6 抗体 (180.2) AC

sc-9964 AC
500 µg/ml, 25% agarose
$416.00

Cdc6 抗体 (180.2) HRP

sc-9964 HRP
200 µg/ml
$316.00

Cdc6 抗体 (180.2) FITC

sc-9964 FITC
200 µg/ml
$330.00

Cdc6 抗体 (180.2) PE

sc-9964 PE
200 µg/ml
$343.00

Cdc6 抗体 (180.2) Alexa Fluor® 488

sc-9964 AF488
200 µg/ml
$357.00

Cdc6 抗体 (180.2) Alexa Fluor® 546

sc-9964 AF546
200 µg/ml
$357.00

Cdc6 抗体 (180.2) Alexa Fluor® 594

sc-9964 AF594
200 µg/ml
$357.00

Cdc6 抗体 (180.2) Alexa Fluor® 647

sc-9964 AF647
200 µg/ml
$357.00

Cdc6 抗体 (180.2) Alexa Fluor® 680

sc-9964 AF680
200 µg/ml
$357.00

Cdc6 抗体 (180.2) Alexa Fluor® 790

sc-9964 AF790
200 µg/ml
$357.00

Cdc6 抗体 (180.2) TRITC

sc-9964 TRITC
200 µg/ml
$320.00

Thanks. I'm working on paraffin-embedded mouse livers. The tissue has proven troublesome in the past (i.e. antibodies working just fine on other paraffin tissues not working at all). Any particular suggestion (i.e. harsher retrieval conditions)? 

Asked by: alberto grn
Thank you for your question. We do not have a specific protocol for mouse liver, but here is a link to our suggested protocol: https://www.scbt.com/resources/protocols/immunoperoxidase-staining We recommend sodium citrate with heat as the primary method, but you can also try any of the other methods in the protocol.
Answered by: Tech Service
Date published: 2019-10-23

Is the antibody suitable for immunofluorescence from paraffin-embedded tissues? If so, what antigen retrieval protocol do you recommend?  Thanks in advance.

Asked by: alberto grn
Thank you for your question. Yes, this antibody, sc-9964, Cdc6 (180.2) is recommended for IF/IHC on paraffin embedded tissue sections. We recommend a starting dilution of 1:50, and suggest antigen retrieval using sodium citrate with heat. You can find the recommended protocol here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-10-22

The antibody sc9964 recognize which part of Cdc6, what is the meaning of 180.2, thank you !

Asked by: LiuLiuM
Thank you for your question. The exact epitope recognized by this monoclonal antibody has not been determined. And 180.2 is the clone designation for this antibody.
Answered by: Technical Support
Date published: 2019-01-26

How can Cdc6 Antibody (180.2): sc-9964 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available phycoerythrin (sc-9964 PE), fluorescein (sc-9964 FITC), Alexa Fluor® 488 (sc-9964 AF488) or Alexa Fluor® 647 (sc-9964 AF647).
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Good antibodyWe used this antibody in WB and got good signal, no complaints.
Date published: 2018-02-19
Rated 5 out of 5 by from Very Specific Antibody!Worked great on cell lysates! Detected signal in untreated and was upregulated in treated cells.
Date published: 2018-01-31
Rated 4 out of 5 by from Good for westernDetected a predicted size band in mouse tissue samples
Date published: 2017-09-29
Rated 5 out of 5 by from good for WBi recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:1000 dilution),the band is strong
Date published: 2017-08-15
Rated 4 out of 5 by from Faint but distinct bands on WBProduced faint but distinct bands on WB. used the ab at 1:1000 dilution
Date published: 2017-07-03
Rated 5 out of 5 by from Good resultsWe use this product the WB dilution of 1:500 had a strong signal.
Date published: 2017-04-10
Rated 5 out of 5 by from Wow !! OutstandingFor all applications including western, IHC and IF best cdc6 antibody
Date published: 2017-01-07
Rated 5 out of 5 by from Great for stainingEqually specific results in IHC(P) and in immunofluorescenc.
Date published: 2016-12-23
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Cdc6 抗体 (180.2) is rated 4.9 out of 5 by 14.
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