Date published: 2025-9-6
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Cdc27 Antibody (C-4) - Western Blotting - Image 358548
Cdc27 抗体 (C-4): sc-13154. MOLT-4 (A), K-562 (B) 和 HeLa (C) 全细胞裂解液中 Cdc27 表达的 Western 印迹分析.
Cdc27 抗体 (C-4): sc-13154. MOLT-4 (A), K-562 (B) 和 HeLa (C) 全细胞裂解液中 Cdc27 表达的 Western 印迹分析.

Cdc27 抗体 (C-4): sc-13154

4.8(8)
寫評論提問

说明书
  • Cdc27抗体(C-4)是小鼠单克隆IgG2a κ, 在15篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Cdc27的氨基酸1-300
  • 抗-Cdc27 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 Cdc27
  • 抗Cdc27抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdc27 (C-4): sc-13154的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Cdc27 抗体 (C-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc27 抗体 (C-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc27抗体（C-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类来源的Cdc27，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。Cdc27 单克隆抗体（C-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc27是细胞周期调控的关键因素，是负责细胞周期后期促进复合物（APC）的重要组成部分，该复合物负责细胞周期后期促进复合物（APC）的泛素化作用和随后细胞周期蛋白B的降解。这种降解对于细胞分裂过程中从中期到后期的过渡至关重要，可确保染色体正确分离并保持基因组稳定。Cdc27和APC的失调会导致细胞无序增殖，并可能引发多种癌症，这凸显了Cdc27在细胞周期调节中的重要性，以及其作为治疗靶点的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc27 抗体 (C-4) 参考文献:

  1. 下调 CDC27 可通过 p21Cip1/Waf1 的积累抑制结直肠癌细胞的增殖。  |  Qiu, L., et al. 2016. Cell Death Dis. 7: e2074. PMID: 26821069
  2. PCBP1/HNRNP E1 通过转录调控 CDC27 保护染色体完整性  |  Link, LA., et al. 2016. Mol Cancer Res. 14: 634-46. PMID: 27102006
  3. CDC27 通过扭曲诱导上皮-间质转化促进胃癌细胞增殖、侵袭和转移  |  Xin, Y., et al. 2018. Cell Physiol Biochem. 50: 501-511. PMID: 30308498
  4. CDC27 在 T 细胞淋巴细胞淋巴瘤中促进肿瘤进展并影响 PD-L1 表达  |  Song, Y., et al. 2020. Front Oncol. 10: 488. PMID: 32391258
  5. CDC27 在癌症中的重要性：分子病理学和临床方面。  |  Kazemi-Sefat, GE., et al. 2021. Cancer Cell Int. 21: 160. PMID: 33750395
  6. CDC27-ODC1 轴促进神经母细胞瘤的转移, 加速铁凋亡并预测不良预后  |  Qiu, L., et al. 2022. Front Oncol. 12: 774458. PMID: 35242701
  7. 一个新基因 CDC27 可导致系统性红斑狼疮并与疾病活动有关。  |  Shang, S., et al. 2022. Front Immunol. 13: 876963. PMID: 35418986
  8. LncRNA TUG1通过上调CDC27和激活PI3K/Akt/mTOR通路促进肺纤维化进展。  |  Qi, F., et al. 2023. Epigenetics. 18: 2195305. PMID: 36994860
  9. 功能和遗传分析揭示了 CDC27 在半面畸形中的作用  |  Song, W., et al. 2024. Int J Mol Sci. 25: PMID: 38731925
  10. 作为急性白血病潜在生物标志物的 CDC27 基因表达模式  |  Pouriafar, Y., et al. 2024. Mol Biol Rep. 51: 865. PMID: 39073611

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc27 抗体 (C-4)

sc-13154
200 µg/ml
$316.00

Cdc27 (C-4): m-IgG Fc BP-HRP 套装

sc-528274
200 µg Ab; 10 µg BP
$354.00

Cdc27 (C-4): m-IgGκ BP-HRP 套装

sc-520613
200 µg Ab, 40 µg BP
$354.00

Cdc27 抗体 (C-4) AC

sc-13154 AC
500 µg/ml, 25% agarose
$416.00

Cdc27 抗体 (C-4) HRP

sc-13154 HRP
200 µg/ml
$316.00

Cdc27 抗体 (C-4) FITC

sc-13154 FITC
200 µg/ml
$330.00

Cdc27 抗体 (C-4) PE

sc-13154 PE
200 µg/ml
$343.00

Cdc27 抗体 (C-4) Alexa Fluor® 488

sc-13154 AF488
200 µg/ml
$357.00

Cdc27 抗体 (C-4) Alexa Fluor® 546

sc-13154 AF546
200 µg/ml
$357.00

Cdc27 抗体 (C-4) Alexa Fluor® 594

sc-13154 AF594
200 µg/ml
$357.00

Cdc27 抗体 (C-4) Alexa Fluor® 647

sc-13154 AF647
200 µg/ml
$357.00

Cdc27 抗体 (C-4) Alexa Fluor® 680

sc-13154 AF680
200 µg/ml
$357.00

Cdc27 抗体 (C-4) Alexa Fluor® 790

sc-13154 AF790
200 µg/ml
$357.00

Can Cdc27 (C-4): sc-13154 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Cdc27 (C-4): sc-13154 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from good productThis is a good product, pretty clean with only one non-specific band on U2OS cell line WB.
Date published: 2017-12-26
Rated 5 out of 5 by from Excellent ResultI used Cdc27 for my WB. It gave me excellent result. I got very nice strong bands.
Date published: 2017-09-29
Rated 5 out of 5 by from satisfactorilyWe have data demonstrating that Cdc16 and Cdc27 are components of APC, and that ubiquitination of cyclin B leads to the degradation of the cyclin B / Cdk complex. It is very gratifying that the results of our experiments have been satisfactorily achieved
Date published: 2017-02-02
Rated 5 out of 5 by from Good results obtained by immunofluorescenceGood results obtained by immunofluorescence when staining for mouse testis. -SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from This antibody was found to be suitable forThis antibody was found to be suitable for use in flow cytometry applications using fixed and permeabolized K-562 cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Great antibody for human and rat immunoprecipitation, published using human and mouse cerebellar branules, HeLa and HEK293 cell lines. -SCBT Publication Review
Date published: 2015-03-01
Rated 5 out of 5 by from Strong band at correct molecular weight observedStrong band at correct molecular weight observed by Western Blot with K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-02
Rated 4 out of 5 by from Good nuclear staining of surface epithelialGood nuclear staining of surface epithelial cells of formalin fixed paraffin embedded (PPFE) human esophagus tissue. -SCBT QC
Date published: 2014-11-13
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Cdc27 抗体 (C-4) is rated 4.8 out of 5 by 8.
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