CD74抗体 (2X31) 推荐用于 mouse, rat 和 human 来源的CD74 WB, IP, IF, IHC(P) 和 FCM检测

CD74 抗体 (2X31): sc-70781

CD74抗体 (2X31) is rated 3.0 out of 5 by 2.
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说明书

    • CD74抗体(2X31)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
    • raised against SU-DHL-4 lymphoma cells
    • 推荐用于 mouse, rat 和 human 来源的CD74 WB, IP, IF, IHC(P) 和 FCM检测
    • 看 CD74 (LN-2): sc-6262,可查看有标记的 CD74 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790

May I know which site/ epitope of CD74 that CD74 (2X31): sc70781 recognises/ binds? I am curious about whether this antibody can detect all forms of CD74 (as I created various CD74 mutants).

Asked by: Lynn93
Thank you for the question. This CD74 specific antibody was raised against SU-DHL-4 lymphoma cells. The exact binding epitope has not been determined exactly, yet. If you are working with CD74 mutants you might also want to check other available CD74 antibodies. We offer two antibodies raised against specific peptides/ rec. protein fragments: sc-47742 was raised against aas 12-28 (h) sc-166047 was raised against aas 7-296 (h) https://www.scbt.com/scbt/browse/cd74-Antik%C3%B6rper/_/N-6thh37
Answered by: Technical Support Europe
Date published: 2018-01-11

Can CD74 (2X31): sc-70781 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, CD74 (2X31): sc-70781 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 3 out of 5 by from Robust bands seen in WB using NAMALWA Robust bands seen in WB using NAMALWA, IB4, J774.A1 and BJAB whole cell lysates. -SCBT QC
Date published: 2015-05-24
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2013-06-01
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