Date published: 2025-9-7
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CD63 Antibody (MX-49.129.5) - Western Blotting - Image 391364
CD63 抗体 (MX-49. 129. 5) Alexa Fluor® 488: sc-5275 AF488. 直接荧光西部印迹分析 CD63 在 CCD-1064Sk 全细胞裂解液中的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
CD63 抗体 (MX-49. 129. 5) Alexa Fluor® 488: sc-5275 AF488. 直接荧光西部印迹分析 CD63 在 CCD-1064Sk 全细胞裂解液中的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

CD63 抗体 (MX-49.129.5): sc-5275

4.5(56)
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说明书
  • CD63 抗体 MX-49.129.5 是小鼠单克隆 IgG1 κ，CD63抗体, 在612篇文献中引用，规格为200 µg/ml
  • 针对全长CD63，human起源
  • CD63 抗体 (MX-49.129.5) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 CD63
  • 抗CD63抗体（MX-49.129.5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取CD63 (MX-49.129.5): sc-5275的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD63 抗体 (MX-49.129.5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD63 抗体 (MX-49.129.5) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

CD63抗体（MX-49.129.5）是一种针对全长人类CD63蛋白开发的小鼠单克隆IgG1 kappa轻链抗体。CD63单克隆抗体（MX-49.129.5）可可靠地检测多种物种（包括小鼠、大鼠和人类）的CD63，推荐用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）等应用。CD63，也被称为LAMP-3、黑色素瘤相关抗原ME491和TSPAN30，是跨膜蛋白家族的重要成员，通过与特定整合素的相互作用，促进细胞粘附、迁移和信号转导等关键过程。在功能上，CD63在吞噬活动和溶酶体与吞噬体的融合中发挥着重要作用，而吞噬体与吞噬体的融合对于细胞内降解和回收机制至关重要。CD63在活化的血小板、单核细胞和巨噬细胞上显著表达，在粒细胞、T细胞和B细胞上也有较低水平的表达，这表明它广泛参与免疫反应。血小板活化时，CD63会动态地转移到细胞膜上，这凸显了其在细胞信号传导和免疫功能中的重要性。作为TM4超家族白细胞糖蛋白的成员，CD63与CD9、CD37和CD53等蛋白质具有相同的结构特征，它们都具有四个跨膜结构域。值得注意的是，CD63的缺陷与Hermansky-Pudlak综合征有关，这强调了其在正常生理过程中的关键作用。CD63 (MX-49.129.5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，为研究人员提供了满足不同实验需求的多功能选择。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD63 抗体 (MX-49.129.5) 参考文献:

  1. 适配复合物 AP-3 在将野生型和突变型 CD63 靶向溶酶体方面的作用  |  Rous, BA., et al. 2002. Mol Biol Cell. 13: 1071-82. PMID: 11907283
  2. CD63 在 CCR5 介导的 1 型人类免疫缺陷病毒感染巨噬细胞中的潜在作用。  |  von Lindern, JJ., et al. 2003. J Virol. 77: 3624-33. PMID: 12610138
  3. 四泛素 CD63 可增强 H, K-ATPase beta-亚基的内化。  |  Duffield, A., et al. 2003. Proc Natl Acad Sci U S A. 100: 15560-5. PMID: 14660791
  4. 多肽 YY 和神经肽 Y 通过调控 CD63, 基质金属蛋白酶-3 和 Cdc42 的活性，诱导小肠细胞表达绒毛蛋白, 降低粘附性并增强迁移。  |  Lee, M., et al. 2005. J Biol Chem. 280: 125-36. PMID: 15504731
  5. CD63 作为与活化相关的 T 细胞成本刺激元件。  |  Pfistershammer, K., et al. 2004. J Immunol. 173: 6000-8. PMID: 15528334
  6. CD63 可调节固定纤维蛋白原上血小板的扩散和酪氨酸磷酸化。  |  Israels, SJ. and McMillan-Ward, EM. 2005. Thromb Haemost. 93: 311-8. PMID: 15711748
  7. 大鼠肥大细胞抗原 AD1（与人类 CD63 或黑色素瘤抗原 ME491 同源）在培养的其他细胞中也有表达。  |  Nishikata, H., et al. 1992. J Immunol. 149: 862-70. PMID: 1634775
  8. 结构独特的新型白细胞表面糖蛋白家族，包括 CD9, CD37, CD53 和 CD63。  |  Horejsí, V. and Vlcek, C. 1991. FEBS Lett. 288: 1-4. PMID: 1879540
  9. CD63通过IRE-IRP系统受铁调节，对于细胞外囊泡分泌铁蛋白非常重要。  |  Pols, MS. and Klumperman, J. 2009. Exp Cell Res. 315: 1584-92. PMID: 18930046
  10. CD63/Pltgp40：与阶段特异性黑色素瘤相关抗原 ME491 相同的血小板活化抗原。  |  Azorsa, DO., et al. 1991. Blood. 78: 280-4. PMID: 2070066
  11. 差异蛋白质组学不支持CD9、CD81或CD63在蛋白质分选到细胞外囊泡中的普遍作用。  |  Song, Z., et al. 2020. Molecules. 25: PMID: 33261145
  12. TIMP1、CD63和β1-整合素的特性及其在癌症中的相互作用对功能的影响。  |  Yanatori, I., et al. 2021. Blood. 138: 1490-1503. PMID: 34265052

订购信息

产品名称产品编号规格价格数量收藏夹

CD63 抗体 (MX-49.129.5)

sc-5275
200 µg/ml
$316.00

CD63 (MX-49.129.5): m-IgG Fc BP-HRP 套装

sc-526477
200 µg Ab; 10 µg BP
$354.00

CD63 (MX-49.129.5): m-IgG1 BP-HRP 套装

sc-531850
200 µg Ab; 20 µg BP
$354.00

CD63 抗体 (MX-49.129.5) AC

sc-5275 AC
500 µg/ml, 25% agarose
$416.00

CD63 抗体 (MX-49.129.5) HRP

sc-5275 HRP
200 µg/ml
$316.00

CD63 抗体 (MX-49.129.5) FITC

sc-5275 FITC
200 µg/ml
$330.00

CD63 抗体 (MX-49.129.5) PE

sc-5275 PE
200 µg/ml
$343.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 488

sc-5275 AF488
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 546

sc-5275 AF546
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 594

sc-5275 AF594
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 647

sc-5275 AF647
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 680

sc-5275 AF680
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 790

sc-5275 AF790
200 µg/ml
$357.00

CD63 抗体 (MX-49.129.5) TRITC

sc-5275 TRITC
200 µg/ml
$320.00

CD63 抗体 (MX-49.129.5) Alexa Fluor® 405

sc-5275 AF405
200 µg/ml
$357.00

A number of reviewers mentioned good western detection of CD63 from non-reducing exosomes. Does the antibody work only on non-reducing CD63? What would be the purpose of running non-reducing exosomes on SDS-PAGE? Thanks!

Asked by: Harley
Thank you for your question CD63 (MX-49.129.5) antibody can work for reducing and non-reducing conditions . https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-11-16

I am researching secreted exosomes, generated from intra-luminal vesicles present in endolysosomes. Would the epitope of this antibody be available to this antibody in this sample?

Asked by: Izaak CW
Thank you for your question. CD63 Antibody (MX-49.129.5): sc-5275 was raised against full length CD63 of human origin. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is not known.
Answered by: Tech Support Europe
Date published: 2023-05-25

Can this antibody be used for flow cytometry? And if possible, what is the optimal concentration for this technique?

Asked by: Alan Hipólito Juárez Sola
Thank you for your question. Anti-CD63 Antibody (MX-49.129.5) is recommended for detection of CD63 of mouse, rat and human origin by flow cytometry. We recommen to use 1 μg per 1 x 106 cells.
Answered by: Tech Support Europe
Date published: 2022-09-02

¿Este anticuerpo puede utilizarse para citometría de flujo? y de ser posible ¿Cuál es la concentración optima para dicha tecnica?

Asked by: Alan Hipólito Juárez Sola
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

Hello, I would like to use this antibody to detect CD63 in fetal bovine serum. Is there any data available showing the corss reactivity with bovine CD63? Thank you!

Asked by: Vida
Thank you for your question. BLAST analysis shows an 85% sequence homology between the full length sequence of human CD63 and bovine CD63, which is lower than our minimum percentage for species reactivity recommendation. Unfortunately, we do not characterize the exact binding site of this monoclonal antibody and we have not tested it with bovine samples so it is uncertain that the antibody will react with the bovine target. If you have additional questions, please contact Technical Support by calling 1800-457-3801 option 2, by emailing scbt@scbt.com or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Blake J
Date published: 2021-07-19

Does this antibody (CD63 Antibody (MX-49.129.5) recognize a cytosolic or luminal/extracellular domain of CD63? i.e has it been epitope mapped?

Asked by: Wyatt
Thank you for your question. CD63 Antibody (MX-49.129.5): sc-5275 has been raised against full length CD63 of human origin. So far, no epitope mapping has been performed for this antibody, so we cannot say whether it binds the cytosolic or extracellular domain of its target protein.
Answered by: Tech Support Europe
Date published: 2020-07-13

I want to check CD63 in chicken. I want to know this product (sc-5275) is working to chicken or not. Thank you.

Asked by: Yeojin
Thank you for your question. We are not aware of any data or product citations using this antibody for detection of CD63 in chicken.
Answered by: Technical Support
Date published: 2020-04-22

Hemos probado otros anticuerpos anti-CD63 que no reconocen bien CD63 de rata o ratón. Es posible recibir una muestra de prueba para comprobar que este anticuerpo (sc-5275) nos sirve para IF y WB en células, extractos y exosomas de rata y ratón?

Asked by: JavierDG
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-01-31
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Rated 5 out of 5 by from Worked well on human adipose MSCs i.fluorescenceImmunofluorescence Staining of Human adipose MSCs. Done with 1:50 dilution in PBS-t 0.1% incubation with 4%NBCS overnight at 4 degrees. Detected with Alexafluor at 1:1000. Confocal image 100x Mag-oil immersion
Date published: 2025-03-07
Rated 3 out of 5 by from discreto funzionamentoHo utilizzato questo anticorpo per valutarne l'espressione nella specie: cane e ho ottenuto un discreto risultato. Dovrò comunque ottimizzare la concentrazione dell'anticorpo
Date published: 2023-07-14
Rated 5 out of 5 by from Westernblottingうまくいきました。東明サイエンス経由で、サンプルをお願いしたところすぐに対応していただき ありがとうございました。Westernblottingにて良いデータがでましたので、 画像を添付いたします。
Date published: 2023-06-13
Rated 5 out of 5 by from WesternblotAntibody works well (1:200 dilution) in westernblot to detect CD63 in airway epithelial exosomes
Date published: 2023-02-09
Rated 5 out of 5 by from Great for exosomesThis antibody worked really well on non-reducing exosome samples.
Date published: 2022-05-10
Rated 5 out of 5 by from For exosome marker from rat plasmaWe have used this antibody as a marker for exosome from rat plasma. It works very good for western blotting in unreduction-SDS-PAGE.
Date published: 2021-07-01
Rated 5 out of 5 by from greatThis antibody have very specific binding to exosome.
Date published: 2020-03-21
Rated 5 out of 5 by from Works very well in Western Blot for exosomesWe routinely use the antibody at 1:2000 dilution for our Western Blot to detect exosomes. The antibody gives a very strong and reliable signal, I can strongly recommend it.
Date published: 2020-03-12
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CD63 抗体 (MX-49.129.5) is rated 4.5 out of 5 by 56.
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