Date published: 2025-9-6
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CD38 Antibody (H-11) - Western Blotting - Image 154717
CD38 抗体 (H-11): sc-374650. CCRF-CEM (A) 和 DU 145 (B) 全细胞裂解液中 CD38 表达的 Western 印迹分析.
CD38 抗体 (H-11): sc-374650. CCRF-CEM (A) 和 DU 145 (B) 全细胞裂解液中 CD38 表达的 Western 印迹分析.

CD38 抗体 (H-11): sc-374650

4.5(16)
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说明书
  • CD38 抗体 H-11 是小鼠单克隆 IgG1 κ，CD38抗体, 在23篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD38的氨基酸1-170
  • CD38 抗体 (H-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CD38
  • 抗CD38抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD38 (H-11): sc-374650的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD38 抗体 (H-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD38 抗体 (H-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CD38抗体（H-11）是一种IgG1κ小鼠单克隆CD38抗体（也被称为ADPRC 1抗体、cADPr水解酶1抗体或环ADP-核糖水解酶1抗体），它通过WB、IP、IF、IHC(P)和ELISA技术检测小鼠、大鼠和人类的CD38蛋白。CD38抗体（H-11）既可以以非偶联抗CD38抗体的形式存在，也可以以多种偶联形式的抗CD38抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD38是一种II型整体膜糖蛋白，存在于早期B和T细胞谱系以及活化的B和T细胞上，但在大多数成熟的静息外周淋巴细胞中缺失。CD38也存在于胸腺细胞、前B细胞、生发中心B细胞、有丝分裂原活化的T细胞、单核细胞和Ig分泌型浆细胞上。在T淋巴细胞中，CD38起NAD糖苷水解酶的作用。在造血细胞上，CD38诱导成熟T和B细胞的活化、增殖和分化，并介导髓系和淋巴系祖细胞的凋亡。除了作为信号受体外，CD38还是一种能够产生几种动员钙的代谢物的酶，包括环腺苷二磷酸核糖（cADPR）。CD38还在维持优先有助于维持免疫耐受的不变NK T（iNKT）细胞亚群的存活中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD38 抗体 (H-11)

sc-374650
200 µg/ml
$316.00

CD38 (H-11): m-IgG Fc BP-HRP 套装

sc-527497
200 µg Ab; 10 µg BP
$354.00

CD38 (H-11): m-IgG1 BP-HRP 套装

sc-532870
200 µg Ab; 20 µg BP
$354.00

CD38 抗体 (H-11) AC

sc-374650 AC
500 µg/ml, 25% agarose
$416.00

CD38 抗体 (H-11) HRP

sc-374650 HRP
200 µg/ml
$316.00

CD38 抗体 (H-11) FITC

sc-374650 FITC
200 µg/ml
$330.00

CD38 抗体 (H-11) PE

sc-374650 PE
200 µg/ml
$343.00

CD38 抗体 (H-11) Alexa Fluor® 488

sc-374650 AF488
200 µg/ml
$357.00

CD38 抗体 (H-11) Alexa Fluor® 546

sc-374650 AF546
200 µg/ml
$357.00

CD38 抗体 (H-11) Alexa Fluor® 594

sc-374650 AF594
200 µg/ml
$357.00

CD38 抗体 (H-11) Alexa Fluor® 647

sc-374650 AF647
200 µg/ml
$357.00

CD38 抗体 (H-11) Alexa Fluor® 680

sc-374650 AF680
200 µg/ml
$357.00

CD38 抗体 (H-11) Alexa Fluor® 790

sc-374650 AF790
200 µg/ml
$357.00

Hi there, do you offer small sample of CD38 Antibody (H-11), sc-374650 to purchase, say 20 ul? We would like to do some quick tests? Thanks.

Asked by: Jiaheng
Thank you for your question. With the purchase of a primary antibody you can receive antibody samples . Samples are 10ug/50ul. To request a quote please contact technical service at scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-05-13

Can this antibody be used for flow cytometry?

Asked by: vino
Thank you for your question. CD38 Antibody (H-11): sc-374650 is also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for FCM applications.
Answered by: Tech Support Europe
Date published: 2022-09-27

Can this antibody be used in immunofluorescence?

Asked by: Paul Zhou
Thank you for your question. This antibody is guaranteed to work by immunofluorescence when using it under recommended conditions and sample types.
Answered by: Technical Support
Date published: 2022-09-27

Can this antibody be used for IHC/IF in mouse tissue?

Asked by: Darlan
Thank for your tissue. The CD38 (H-11)is recommended for detection of CD38 of mouse origin by WB, IP, IF, IHC(P) and ELISA. Please contact technical service if you need additional information.
Answered by: SCBT Heidelberg Support
Date published: 2022-09-27

Can this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, this utrophin antibody sc-374650 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-18

Does this antibody cross-react with mouse CD38? RAW267.1 cells are of mouse origin, you show a Blot of mouse CD38.

Asked by: Neel
Thank you for inquiring with us. We do support our CD38 (H-11) antibody, sc-374650, for detection of mouse CD38 based on positive Western Blot data with RAW267.1 whole cell lysate.
Answered by: Technical Service
Date published: 2016-10-13
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Rated 1 out of 5 by from 非特異的バンドが多く、ターゲットが分かりづらい１ヶ月前にサンプルを提供してもらい、マウスの肝臓ライセートでWBを試しました。nonspecific bandが多く、Targetの38kDバンドが薄くてはっきりしません。なぜか50kDに濃いバンドが出ます。細胞の発現量にもよるのでしょうが、上手くいきませんでした。
Date published: 2019-08-27
Rated 5 out of 5 by from Really great antibody...... highly recommend itI tested the antibody on western blot using mouse lysates. Gave a single sharp band at the predicted molecular weight.
Date published: 2018-05-28
Rated 1 out of 5 by from can not detect specific band by WBit can not detect a specific band by WB in our lab.
Date published: 2017-09-18
Rated 5 out of 5 by from IF is goodFluorescence effect is very good , small kind of activity is very good,and continue in the future
Date published: 2017-07-23
Rated 5 out of 5 by from positive resultThe WB result is positive use mosue ovary tissue extracts (loaded 50ug),1:500 diluted the antiboy,the result gave the strong band at 45kda
Date published: 2017-07-08
Rated 5 out of 5 by from strong bandobserved strong band by Western Blot using human platelet extracts, no background .
Date published: 2017-04-08
Rated 5 out of 5 by from western blotI used it to do western blot, It have signal in mouse testis, Reliable!
Date published: 2017-03-05
Rated 5 out of 5 by from St5ong WB and IHCVery clear, strong antibody for western blot applications; also great IHC staining data
Date published: 2017-02-01
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CD38 抗体 (H-11) is rated 4.5 out of 5 by 16.
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