Date published: 2025-9-8

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CD177 抗体 (C-1): sc-374291

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说明书
  • CD177抗体(C-1)是小鼠单克隆IgG2a (kappa轻链),规格为200 µg/ml
  • 针对human起源的CD177内部区域内的氨基酸27-247进行
  • CD177 抗体 (C-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 CD177
  • 抗CD177抗体(C-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD177 (C-1): sc-374291免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 CD177 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD177 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

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    関連項目

    CD177抗体(C-1)是一种IgG2a κ型小鼠单克隆CD177抗体,可通过WB、IP、IF、IHC(P)和ELISA技术检测人源CD177。CD177抗体(C-1)既可以是非偶联的抗CD177抗体形式,也可以是多种偶联形式的抗CD177抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真性红细胞增多症(PV)是一种导致所有髓系细胞系过度增生的肿瘤性干细胞疾病,患者会过度表达细胞表面抗原CD177,也称为NB1。产量增加,尤其是红细胞的增加,会增加全血粘度,导致血管闭塞和缺血。与健康个体或其他红细胞增多相关疾病患者相比,PV患者CD177表达显著上调,因此分析该蛋白对于PV的诊断和机制研究具有重要意义。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    订购信息

    产品名称产品编号规格价格数量收藏夹

    CD177 抗体 (C-1)

    sc-374291
    200 µg/ml
    $316.00

    CD177 (C-1): m-IgG Fc BP-HRP 套装

    sc-529712
    200 µg Ab; 10 µg BP
    $354.00

    CD177 (C-1): m-IgGκ BP-HRP 套装

    sc-522688
    200 µg Ab, 40 µg BP
    $354.00

    CD177 抗体 (C-1) AC

    sc-374291 AC
    500 µg/ml, 25% agarose
    $416.00

    CD177 抗体 (C-1) HRP

    sc-374291 HRP
    200 µg/ml
    $316.00

    CD177 抗体 (C-1) FITC

    sc-374291 FITC
    200 µg/ml
    $330.00

    CD177 抗体 (C-1) PE

    sc-374291 PE
    200 µg/ml
    $343.00

    CD177 抗体 (C-1) Alexa Fluor® 488

    sc-374291 AF488
    200 µg/ml
    $357.00

    CD177 抗体 (C-1) Alexa Fluor® 546

    sc-374291 AF546
    200 µg/ml
    $357.00

    CD177 抗体 (C-1) Alexa Fluor® 594

    sc-374291 AF594
    200 µg/ml
    $357.00

    CD177 抗体 (C-1) Alexa Fluor® 647

    sc-374291 AF647
    200 µg/ml
    $357.00

    CD177 抗体 (C-1) Alexa Fluor® 680

    sc-374291 AF680
    200 µg/ml
    $357.00

    CD177 抗体 (C-1) Alexa Fluor® 790

    sc-374291 AF790
    200 µg/ml
    $357.00

    Is C-1 the name of clone, such as 1171a or Y127? Can I test this antibody to detect surface mouse CD177 on HEK293T cells that overexpresses mouse CD177? I already tested the expression of mCD177 using other anti-mouse CD177 antibody.

    Asked by: Anonymous
    Thank you for your question. C-1 is the clone name of this antibody. The CD177 Antibody (C-1): sc-374291 is only recommended for detection of CD177 of human origin by WB, IP, IF, IHC(P) and ELISA. It would not recognize murine CD177. A mouse-specific anti-CD177 antibody such as CD177 Antibody (C-5): sc-376329 would be suitable instead.
    Answered by: Technical Support Europe
    Date published: 2020-10-16

    Can CD177 (C-1): sc-374291 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: AbPolly
    Thank you for your question. Yes, CD177 (C-1): sc-374291 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 5 out of 5 by from Satisfactory Western Blot data of CD177 expressionSatisfactory Western Blot data of CD177 expression in human PBL whole cell lysate. -SCBT QC
    Date published: 2015-02-26
    Rated 5 out of 5 by from Nice Western Blot data of CD177 expressionNice Western Blot data of CD177 expression in PMA treated human PBL whole cell lysate. -SCBT QC
    Date published: 2013-10-19
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    CD177 抗体 (C-1) is rated 5.0 out of 5 by 2.
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