Date published: 2025-9-6
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CBP/KAT3A/CREBBP Antibody (C-1) - Western Blotting - Image 136668
CBP 抗体 (C-1): sc-7300. CBP 在 Jurkat (A) 和 A-431 (B) 核提取物中表达的 Western 印迹分析.
CBP 抗体 (C-1): sc-7300. CBP 在 Jurkat (A) 和 A-431 (B) 核提取物中表达的 Western 印迹分析.

CBP/KAT3A/CREBBP 抗体 (C-1): sc-7300

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说明书
  • CBP/KAT3A/CREBBP 抗体 C-1 是小鼠单克隆 IgG1 κ，CBP/KAT3A/CREBBP抗体, 在141篇文献中引用，规格为200 µg/ml
  • 特定于原点 human 的 2423-2442 氨基酸之间的表位映射，位于 C-terminus 的 CBP（与相应的鼠序列相同）
  • CBP/KAT3A/CREBBP 抗体 (C-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的CBP p265; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗CBP/KAT3A/CREBBP抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7300 X, 200 µg/0.1 ml)
  • 关于如何获取CBP/KAT3A/CREBBP (C-1): sc-7300的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 CBP/KAT3A/CREBBP 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与CBP/KAT3A/CREBBP 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CBP抗体（C-1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类来源的CBP蛋白，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）。CBP (C-1) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。CBP蛋白（也称为CREBBP或KAT3A）在调节基因表达以响应环状AMP信号方面发挥着关键作用，尤其是通过与cAMP调节增强子（CRE）相互作用。这种增强子是多个转录因子结合的关键调节元件，包括CREB，后者由蛋白激酶A的磷酸化激活。磷酸化CREB与CBP的结合对于靶基因的转录共激活至关重要，这凸显了CBP在调节细胞对激素信号的反应中的重要性。CBP与另一种转录共激活因子p300具有显著的同源性，这表明这两种蛋白质都属于一个保守家族，在基因调控中发挥着重要作用。CBP与各种转录因子的相互作用以及参与关键细胞过程，凸显了其在分子生物学中的重要性，以及对理解与基因表达失调相关的疾病的潜在意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CBP/KAT3A/CREBBP 抗体 (C-1) 参考文献:

  1. CREB结合蛋白调节多Q病果蝇模型中的重复不稳定性  |  Jung, J. and Bonini, N. 2007. Science. 315: 1857-9. PMID: 17332375
  2. Wnt/beta-catenin/CBP 信号维持小鼠胚胎干细胞的长期多能性。  |  Miyabayashi, T., et al. 2007. Proc Natl Acad Sci U S A. 104: 5668-73. PMID: 17372190
  3. CREB结合蛋白（CBP）/p300在呼吸道上皮肿瘤发生中的作用  |  Karamouzis, MV., et al. 2007. Cell Res. 17: 324-32. PMID: 17372613
  4. CBP/p300溴结构域抑制剂开发的最新进展。  |  Xiong, Y., et al. 2020. Curr Med Chem. 27: 5583-5598. PMID: 31364510
  5. p300/CBP是肝能量稳态和肝纤维化的关键营养传感器。  |  Waddell, AR., et al. 2021. Cancers (Basel). 13: PMID: 34201346
  6. P300/CBP通过间歇性缺氧调节HIF-1依赖性交感神经激活和高血压。  |  Tang, X., et al. 2023. Biomedicines. 11: PMID: 38002029
  7. 腺病毒 E1A 相关 300-kD 蛋白（p300）的分子克隆和功能分析揭示了一种具有转录适配器特性的蛋白质。  |  Eckner, R., et al. 1994. Genes Dev. 8: 869-84. PMID: 7523245
  8. E1A 肿瘤蛋白靶向的转录适配蛋白家族。  |  Arany, Z., et al. 1995. Nature. 374: 81-4. PMID: 7870178
  9. 腺病毒 E1A 相关蛋白 p300 作为转录共激活因子 CBP 的功能同源物。  |  Lundblad, JR., et al. 1995. Nature. 374: 85-8. PMID: 7870179
  10. E1A 相关 p300 和 CREB 相关 CBP 属于一个保守的辅助激活子家族。  |  Arany, Z., et al. 1994. Cell. 77: 799-800. PMID: 8004670
  11. 磷酸化的 CREB 能与核蛋白 CBP 特异性结合。  |  Chrivia, JC., et al. 1993. Nature. 365: 855-9. PMID: 8413673
  12. 核蛋白 CBP 是转录因子 CREB 的辅助激活因子。  |  Kwok, RP., et al. 1994. Nature. 370: 223-6. PMID: 7913207

订购信息

产品名称产品编号规格价格数量收藏夹

CBP/KAT3A/CREBBP 抗体 (C-1)

sc-7300
200 µg/ml
$316.00

CBP/KAT3A/CREBBP (C-1): m-IgGκ BP-HRP 套装

sc-551061
200 µg Ab; 40 µg BP
$354.00

CBP/KAT3A/CREBBP 抗体 (C-1) X

sc-7300 X
200 µg/0.1 ml
$316.00

CBP/KAT3A/CREBBP 抗体 (C-1) AC

sc-7300 AC
500 µg/ml, 25% agarose
$416.00

CBP/KAT3A/CREBBP 抗体 (C-1) HRP

sc-7300 HRP
200 µg/ml
$316.00

CBP/KAT3A/CREBBP 抗体 (C-1) FITC

sc-7300 FITC
200 µg/ml
$330.00

CBP/KAT3A/CREBBP 抗体 (C-1) PE

sc-7300 PE
200 µg/ml
$343.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 488

sc-7300 AF488
200 µg/ml
$357.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 546

sc-7300 AF546
200 µg/ml
$357.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 594

sc-7300 AF594
200 µg/ml
$357.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 647

sc-7300 AF647
200 µg/ml
$357.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 680

sc-7300 AF680
200 µg/ml
$357.00

CBP/KAT3A/CREBBP 抗体 (C-1) Alexa Fluor® 790

sc-7300 AF790
200 µg/ml
$357.00

CBP/KAT3A/CREBBP (C-1) 中和勝肽

sc-7300 P
100 µg/0.5 ml
$68.00

Do you have a directly conjugated isotype control for this antibody. I bought sc-7300 AF594 and need a proper control to show that the positive signal is not a non-specific binding

Asked by: zhangll
Thank you for your question. The appropriate isotype control for AF594-conjugated sc-7300 is normal mouse IgG1 Alexa Fluor® 594: sc-516620.
Answered by: Tech Support Europe
Date published: 2020-09-07

I recently purchased this antibody. However, I need to know if this antibody cross-reacts with the closely related p300 protein (KAT3B aka EP300) in human cells. A rapid and accurate response would be greatly appreciated. Thanks, Isaac

Asked by: IHilton
Thank you for your question. This mouse monoclonal antibody, CBP (C-1): sc-7300, is specific for CBP (CREB-binding protein). We have not specifically tested this clone for potential cross-reactivity with KAT3B aka EP300, however this antibody has been validated for specificity using our human siRNA for knockdown against the human gene target, CBP siRNA (h): sc-29244.
Answered by: Technical Support
Date published: 2019-06-24

antibody Cat#sc-7300: how do I get in possession of the 10ug sample as advertised on your website? Thanks

Asked by: Seia
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, CBP Antibody (C-1): sc-7300. Please contact our Technical Service Department and we would be happy to arrange a complimentary shipment for you. You may call us toll free at (800) 457-3801 option 2, or email scbt@scbt.com, or use our live chat!
Answered by: Technical Support
Date published: 2017-01-25

Is there any data showing this antibody is specific to CBP?

Asked by: jenni
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to CBP.
Answered by: Technical Support 13
Date published: 2020-08-10
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Rated 5 out of 5 by from Looks visible and greatThis antibody works well with transfer buffer that included 0.1%SDS and only 10% methanol. Use for incubation at 1:500
Date published: 2018-12-12
Rated 5 out of 5 by from Works in ChIP analysisIn ChIP analysis, tThis antibody could detect forskolin-dependent binding of CBP to c-Fos promoter in HEK293FT cells.
Date published: 2018-10-29
Rated 1 out of 5 by from not working for me in IFAI was unfortunately not successful in getting a signal with 96 well plate probed with recombinant CBP tagged protein O/N at 4C using a fluorescent secondary antibody for 1hr. Antibodies were concentrated as high as 1:50 in PBS-BSA and incubated for 1-2hrs at RT. I used an anti-CBP from a different vendor that gave me a specific signal. This was also tried on fixed cell lines expressing CBP on the surface with negative results as well.
Date published: 2018-10-24
Rated 5 out of 5 by from Good Product 2The antibody worked well at 1:500 dilution using mouse brain tissue extract.
Date published: 2018-08-29
Rated 2 out of 5 by from なんとも言えない過剰発現したものには当たりましたが、内在性のものはdetectできませんでした。当たりが弱い気はしました。
Date published: 2018-07-03
Rated 2 out of 5 by from not satisfied for IFI tested this AB in IF (range 1/20 to 1/100) both in cells and brain E16 mouse the signal was really low (PFA fixation in both case)
Date published: 2018-03-22
Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 3 out of 5 by from It works for western blotting.It detected a faint band with the expected size in nuclear extracts of immortalized HFFs, but not in total protein extracts (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date published: 2018-02-23
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CBP/KAT3A/CREBBP抗体 (C-1) is rated 4.3 out of 5 by 37.
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