Date published: 2025-9-7

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cathepsin L 抗体 (33/2): sc-32320

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说明书
  • cathepsin L 抗体 33/2 是小鼠单克隆 IgG1 κ,cathepsin L抗体, 在21篇文献中引用,规格为200 µg/ml
  • 针对human原cathepsin L
  • cathepsin L 抗体 (33/2) 推荐用于WB, IP, IF 和 IHC(P),检测human,以及在较小程度上,检测mouse 和 rat来源的cathepsin L and procathepsin L
  • 抗cathepsin L抗体(33/2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cathepsin L (33/2): sc-32320免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 cathepsin L 抗体 (33/2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cathepsin L 抗体 (33/2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Cathepsin L 抗体(33/2)是一种小鼠单克隆 IgG1 kappa 轻链抗体,用于检测 cathepsin L 蛋白,也称为 CTSL、主要排泄蛋白(MEP)或 CATL。抗组织蛋白酶 L 抗体 (33/2) 针对人类前组织蛋白酶 L 产生,在人类样本中与组织蛋白酶 L 及其前酶形式均表现出强烈的反应性,并与小鼠和大鼠物种存在交叉反应,因此非常适合用于蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组织化学 (IHCP)。Cathepsin L是一种重要的溶酶体半胱氨酸蛋白酶,参与胶原蛋白、弹性蛋白和α-1蛋白酶抑制剂等蛋白质的细胞内降解,在细胞代谢和蛋白质分解代谢中发挥着重要作用。这种蛋白酶对于维持细胞内环境至关重要,并参与多种生理过程,包括组织重塑和免疫反应。 组织蛋白酶 L (33/2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物,为不同的检测方法提供了灵活性。作为蛋白酶C1家族的一员,组织蛋白酶L以二聚体形式存在,由来自单个前体蛋白的二硫键连接的重链和轻链组成,从而确保研究中可靠且一致的性能。无论是研究蛋白质降解途径、细胞代谢还是蛋白酶在疾病中的作用,组织蛋白酶L单克隆抗体(33/2)都能提供推进科学发现所需的特异性和多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cathepsin L 抗体 (33/2) 参考文献:

  1. 骨骼发育过程中胰蛋白酶的表达  |  Söderström, M., et al. 1999. Biochim Biophys Acta. 1446: 35-46. PMID: 10395917
  2. 人 cathepsin L 启动子的克隆和表征。  |  Bakhshi, R., et al. 2001. Gene. 275: 93-101. PMID: 11574156
  3. 人类 cathepsin L mRNA 的剪接变体显示出不同的表达率。  |  Abudula, A., et al. 2001. Biol Chem. 382: 1583-91. PMID: 11767948
  4. 新型人类凝血酶 L 剪接变体的鉴定和特征描述。  |  Arora, S. and Chauhan, SS. 2002. Gene. 293: 123-31. PMID: 12137950
  5. 鉴定白细胞介素-8 转换酶为 cathepsin L。  |  Ohashi, K., et al. 2003. Biochim Biophys Acta. 1649: 30-9. PMID: 12818188
  6. 手术肺癌中 cathepsin B 和 cathepsin L 的表达, 增殖活性和临床意义。  |  Kayser, K., et al. 2003. Anticancer Res. 23: 2767-72. PMID: 12926111
  7. 骨骼肌中猫蛋白酶 L 基因表达受损与 2 型糖尿病有关。  |  Huang, X., et al. 2003. Diabetes. 52: 2411-8. PMID: 12941783
  8. 人和小鼠前凝血酶 L 的完整核苷酸和推导氨基酸序列。  |  Joseph, LJ., et al. 1988. J Clin Invest. 81: 1621-9. PMID: 2835398
  9. 大鼠 cathepsin L 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin L 抗体 (33/2)

sc-32320
200 µg/ml
$316.00

cathepsin L (33/2): m-IgG Fc BP-HRP 套装

sc-526678
200 µg Ab; 10 µg BP
$354.00

cathepsin L (33/2): m-IgG1 BP-HRP 套装

sc-532051
200 µg Ab; 20 µg BP
$354.00

cathepsin L 抗体 (33/2) AC

sc-32320 AC
500 µg/ml, 25% agarose
$416.00

cathepsin L 抗体 (33/2) HRP

sc-32320 HRP
200 µg/ml
$316.00

cathepsin L 抗体 (33/2) FITC

sc-32320 FITC
200 µg/ml
$330.00

cathepsin L 抗体 (33/2) PE

sc-32320 PE
200 µg/ml
$343.00

cathepsin L 抗体 (33/2) Alexa Fluor® 488

sc-32320 AF488
200 µg/ml
$357.00

cathepsin L 抗体 (33/2) Alexa Fluor® 546

sc-32320 AF546
200 µg/ml
$357.00

cathepsin L 抗体 (33/2) Alexa Fluor® 594

sc-32320 AF594
200 µg/ml
$357.00

cathepsin L 抗体 (33/2) Alexa Fluor® 647

sc-32320 AF647
200 µg/ml
$357.00

cathepsin L 抗体 (33/2) Alexa Fluor® 680

sc-32320 AF680
200 µg/ml
$357.00

cathepsin L 抗体 (33/2) Alexa Fluor® 790

sc-32320 AF790
200 µg/ml
$357.00

What is the recommended titer to use when staining on fixed cells

Asked by: Paige
Thank you for your question.We recommend a starting dilution of 1:50-1:500.
Answered by: BlakeJ
Date published: 2024-09-03

I am using cathepsin L (33/2): sc-32320 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very goodThe Ab was tested for WB, IF and IHC, worked well.
Date published: 2021-11-29
Rated 5 out of 5 by from Excellent IF staining in VeroE6 cellsGreat staining in VeroE6 cells (1:200, methanol fixation) by IF
Date published: 2021-05-04
Rated 4 out of 5 by from good antibodyThis antibody delivered very specific location and moderate signal
Date published: 2018-02-14
Rated 3 out of 5 by from WBThis antibody worked ok for me with 1:200 and ECL prime
Date published: 2017-05-30
Rated 1 out of 5 by from keine Ergebnisse in IHCVor ein paar Wochen Testmenge bestellt. Antikörper hat überhaupt nicht funktioniert. aber Kontrollslides schon. Leider nur Zeitverschwendung.
Date published: 2017-03-08
Rated 5 out of 5 by from Well rounded antibodyGreat antibody all around, really strong for Western blot, IHC and for IF. Smart pick!
Date published: 2017-02-08
Rated 4 out of 5 by from Worked well for immunohistochemical investigationWorked well for immunohistochemical investigation - used 1:1000 dilution on fomalin fixed paraffin embedded temporal lobe sections (human). -SCBT Publication Review
Date published: 2015-05-17
Rated 4 out of 5 by from Clear band (mature form) observed in westernClear band (mature form) observed in western blot analysis of A549 whole cell lysates. -SCBT QC
Date published: 2015-05-02
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cathepsin L抗体 (33/2) is rated 3.9 out of 5 by 11.
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