casein kinase IIα′ Double Nickase Plasmid (h): sc-401148-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- casein kinase IIα′ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- casein kinase IIα′ Double Nickase Plasmid (h) and casein kinase IIα′ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSNK2A2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase IIα′ Double Nickase Plasmid (h) | sc-401148-NIC | 20 µg | $410.00 | |||
casein kinase IIα′ Double Nickase Plasmid (h2) | sc-401148-NIC-2 | 20 µg | $410.00 |
CSNK2A2 encodes the casein kinase II alpha′ catalytic subunit, a constitutively active serine/threonine kinase that partners with regulatory beta subunits to form the CK2 holoenzyme. CK2 phosphorylates a broad set of substrates involved in cell-cycle progression, DNA damage signaling, chromatin regulation, and ribosome biogenesis, and it intersects with pathways such as Wnt/β-catenin, NF-κB, and PI3K/AKT to tune proliferation and stress responses. Through these functions, CSNK2A2 contributes to the maintenance of proteostasis and transcriptional programs that are frequently dysregulated in cancer and other disorders characterized by altered phosphorylation networks. Perturbation of CK2 activity is also used to study kinase-dependent control of apoptosis, differentiation, and inflammatory signaling in diverse human cell models.
casein kinase IIα′ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSNK2A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSNK2A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSNK2A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSNK2A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.