Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Carbonyl reductase 1 Double Nickase Plasmid (h): sc-402755-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Carbonyl reductase 1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Carbonyl reductase 1 Double Nickase Plasmid (h) and Carbonyl reductase 1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CBR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Carbonyl reductase 1 Antibody (B-11): sc-390554
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Carbonyl reductase 1 Double Nickase Plasmid (h) | sc-402755-NIC | 20 µg | $410.00 | |||
Carbonyl reductase 1 Double Nickase Plasmid (h2) | sc-402755-NIC-2 | 20 µg | $410.00 |
Human CBR1 encodes carbonyl reductase 1, a cytosolic NADPH-dependent oxidoreductase that catalyzes the reduction of reactive aldehydes and ketones to corresponding alcohols. This enzyme contributes to cellular redox homeostasis and detoxification by processing endogenous carbonyl species generated during oxidative stress and lipid peroxidation, as well as diverse xenobiotic substrates. Through these activities, CBR1 intersects with metabolic stress responses and influences cellular sensitivity to carbonyl burden. Altered CBR1 expression or activity has been examined in contexts of inflammation, metabolic dysregulation, and tumor biology where carbonyl stress and drug metabolism pathways can affect phenotype.
Carbonyl reductase 1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CBR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CBR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CBR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CBR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.