Date published: 2025-9-7

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c-Jun 抗体 (G-4): sc-74543

4.5(44)
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说明书
  • c-Jun 抗体 G-4 是小鼠单克隆 IgG1 κ,c-Jun抗体, 在146篇文献中引用,规格为200 µg/ml
  • 免疫human物种的c-Jun的氨基酸1-79
  • c-Jun 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 c-Jun p39
  • 抗c-Jun抗体(G-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74543 X, 200 µg/0.1 ml)
  • 关于如何获取c-Jun (G-4): sc-74543免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是c-Jun Antibody (G-4) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与c-Jun Antibody (G-4) 打包提供(请参阅下面的订购信息)。

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関連項目

c-Jun抗体(G-4)是一种IgG1 κ小鼠单克隆c-Jun抗体(也称为JUN抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的c-Jun蛋白。c-Jun抗体(G-4)既有非偶联抗c-Jun抗体形式,也有多种偶联形式的抗c-Jun抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。属于Jun和Fos癌基因家族的基因编码核蛋白,这些核蛋白与许多转录复合物相关。c-Jun蛋白是转录因子AP-1的主要组成部分,最初显示通过TPA应答元件(TRE)介导佛波酯肿瘤促进剂(TPA)诱导的应答基因的表达。Jun蛋白形成同源二聚体和异源二聚体,这些二聚体结合TRE,而Fos蛋白仅作为与任何Jun蛋白的异源二聚体时才具有活性。Fos/Jun异源二聚体对TRE的亲和力远高于Jun同源二聚体。Ha-Ras增强c-Jun活性并刺激其激活域的磷酸化。Fos/Jun功能的抑制剂称为IP-1,它与Fos和Jun结合,并在cAMP依赖性蛋白激酶A(PKA)诱导的磷酸化后被灭活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Jun 抗体 (G-4) 参考文献:

  1. 蛋白激酶 D 在体外直接靶向 c-jun 中一个新的磷酸化位点的鉴定。  |  Waldron, RT., et al. 2007. Biochem Biophys Res Commun. 356: 361-7. PMID: 17359934
  2. IP-1:Fos/Jun 的主要抑制剂,其活性受磷酸化调节。  |  Auwerx, J. and Sassone-Corsi, P. 1991. Cell. 64: 983-93. PMID: 1900458
  3. Ha-Ras 可增强 c-Jun 的活性并刺激其活化结构域的磷酸化。  |  Binétruy, B., et al. 1991. Nature. 351: 122-7. PMID: 1903181
  4. 人类原癌基因 c-jun 编码一种 DNA 结合蛋白,具有转录因子 AP-1 的结构和功能特性。  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
  5. Fos 复合物和 Fos 相关抗原可识别包含 AP-1 结合位点的序列元素。  |  Franza, BR., et al. 1988. Science. 239: 1150-3. PMID: 2964084
  6. c-Jun调控Litopenaeus vannamei小亚基血蓝蛋白基因的启动子。  |  Yang, P., et al. 2019. Fish Shellfish Immunol. 84: 639-647. PMID: 30366093
  7. c-fos 原癌基因产物的染色质关联和 DNA 结合特性。  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
  8. 癌基因 jun 编码一种类似于 AP-1 的序列特异性反式激活因子。  |  Angel, P., et al. 1988. Nature. 332: 166-71. PMID: 3347253
  9. Fos 蛋白复合物与离体细胞核中的 DNA 相关联,并与 DNA 纤维素结合。  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
  10. NAP1L1在卵巢癌中通过HDGF/C-JUN信号促进肿瘤增殖  |  Xiaohua Zhu,., et al. 2022. BMC Cancer. 22: 339. PMID: 35351053
  11. 调节脂肪细胞分化过程中基因表达的核蛋白复合物:c-fos 的直接参与。  |  Distel, RJ., et al. 1987. Cell. 49: 835-44. PMID: 3555845
  12. c-JUN是hESC向心肌细胞转化过程中的一个障碍。  |  Zhong, H., et al. 2023. Life Sci Alliance. 6: PMID: 37604584

订购信息

产品名称产品编号规格价格数量收藏夹

c-Jun 抗体 (G-4)

sc-74543
200 µg/ml
$316.00

c-Jun (G-4): m-IgG Fc BP-HRP 套装

sc-527044
200 µg Ab; 10 µg BP
$354.00

c-Jun (G-4): m-IgG1 BP-HRP 套装

sc-532417
200 µg Ab; 20 µg BP
$354.00

c-Jun 抗体 (G-4) X

sc-74543 X
200 µg/0.1 ml
$316.00

c-Jun 抗体 (G-4) AC

sc-74543 AC
500 µg/ml, 25% agarose
$416.00

c-Jun 抗体 (G-4) HRP

sc-74543 HRP
200 µg/ml
$316.00

c-Jun 抗体 (G-4) FITC

sc-74543 FITC
200 µg/ml
$330.00

c-Jun 抗体 (G-4) PE

sc-74543 PE
200 µg/ml
$343.00

c-Jun 抗体 (G-4) Alexa Fluor® 488

sc-74543 AF488
200 µg/ml
$357.00

c-Jun 抗体 (G-4) Alexa Fluor® 546

sc-74543 AF546
200 µg/ml
$357.00

c-Jun 抗体 (G-4) Alexa Fluor® 594

sc-74543 AF594
200 µg/ml
$357.00

c-Jun 抗体 (G-4) Alexa Fluor® 647

sc-74543 AF647
200 µg/ml
$357.00

c-Jun 抗体 (G-4) Alexa Fluor® 680

sc-74543 AF680
200 µg/ml
$357.00

c-Jun 抗体 (G-4) Alexa Fluor® 790

sc-74543 AF790
200 µg/ml
$357.00

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-74543 PE), fluorescein (sc-74543 FITC), Alexa Fluor® 488 (sc-74543 AF488) or Alexa Fluor® 647 (sc-74543 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 14
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 5 out of 5 by from 功能很出色!一年前申请了小样,1:800-1000稀释,用于肿瘤细胞WB实验,条带无背景,且很深,强烈推荐给各位!
Date published: 2025-02-15
Rated 5 out of 5 by from c-junI tested this antibody in IHC (1:100) and I am very satisfied.
Date published: 2024-01-16
Rated 5 out of 5 by from Great feturesI checked my protein extracted from mouse C166 cell line and the band shows at expected size and has only one single strong band.
Date published: 2021-12-03
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from SDSPAGESingle and clear band in human cell line during ip 1:1000
Date published: 2018-06-13
Rated 2 out of 5 by from Not goodThis one worked as worse as the previous tested samples, so I will not recommend it.
Date published: 2018-03-30
Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 4 out of 5 by from Good, but needs antigen retrievalWe used this antibody on rat DRG tissue, it worked great at 1:200, but needs citrate antigen retrieval, or it won't work.
Date published: 2017-11-29
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c-Jun 抗体 (G-4) is rated 4.5 out of 5 by 44.
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