Date published: 2025-9-6
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c-Fos Antibody (E-8) - Western Blotting - Image 350697
c-Fos 抗体 (E-8): sc-166940. HeLa (A), MIA PaCa-2 (B), NIH/3T3 (C) 和 A-431 (D) 全细胞裂解液及 NIH/3T3 核提取物 (E) 中 c-Fos 表达的 Western 印迹分析.
c-Fos 抗体 (E-8): sc-166940. HeLa (A), MIA PaCa-2 (B), NIH/3T3 (C) 和 A-431 (D) 全细胞裂解液及 NIH/3T3 核提取物 (E) 中 c-Fos 表达的 Western 印迹分析.

c-Fos 抗体 (E-8): sc-166940

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说明书
  • c-Fos 抗体 E-8 是小鼠单克隆 IgG1 κ，c-Fos抗体, 在171篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的c-Fos的内部区域内的氨基酸128-152之间
  • c-Fos 抗体 (E-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human, zebrafish 和 来源的c-Fos, Fos B, Fra-1 and Fra-2; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗c-Fos抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166940 X, 200 µg/0.1 ml)
  • 关于如何获取c-Fos (E-8): sc-166940的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 c-Fos 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与c-Fos 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加c-Fos抗体，包括Fos B

c-Fos抗体（E-8）是一种IgG1 κ小鼠单克隆c-Fos抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠、人类和斑马鱼的c-Fos, Fos B, Fra-1 和 Fra-2蛋白。c-Fos抗体（E-8）既可作为非偶联抗c-Fos抗体形式提供，也可作为多种偶联形式的抗c-Fos抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Fos癌基因最初是在两个独立的小鼠骨肉瘤病毒分离株和一个禽类肾母细胞瘤病毒中检测到的。其细胞同源物c-Fos编码一种核磷蛋白，该蛋白可被多种因子迅速且瞬时诱导，并作为几个基因的转录调节因子。与形成同源二聚体和异源二聚体并与特定DNA反应元件结合的c-Jun蛋白不同，c-Fos蛋白仅在与Jun基因家族成员结合形成异源二聚体时才具有活性。c-Fos的功能同源物包括Fra-1、Fra-2和Fos B基因。此外，选定的ATF/CREB家族成员可以与Fos和Jun形成亮氨酸拉链二聚体。不同的二聚体对AP-1和CRE位点表现出不同的特异性和亲和力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

c-Fos 抗体 (E-8)

sc-166940
200 µg/ml
$316.00

c-Fos (E-8): m-IgG Fc BP-HRP 套装

sc-527195
200 µg Ab; 10 µg BP
$354.00

c-Fos (E-8): m-IgG1 BP-HRP 套装

sc-532568
200 µg Ab; 20 µg BP
$354.00

c-Fos 抗体 (E-8) X

sc-166940 X
200 µg/0.1 ml
$316.00

c-Fos 抗体 (E-8) AC

sc-166940 AC
500 µg/ml, 25% agarose
$416.00

c-Fos 抗体 (E-8) HRP

sc-166940 HRP
200 µg/ml
$316.00

c-Fos 抗体 (E-8) FITC

sc-166940 FITC
200 µg/ml
$330.00

c-Fos 抗体 (E-8) PE

sc-166940 PE
200 µg/ml
$343.00

c-Fos 抗体 (E-8) Alexa Fluor® 488

sc-166940 AF488
200 µg/ml
$357.00

c-Fos 抗体 (E-8) Alexa Fluor® 546

sc-166940 AF546
200 µg/ml
$357.00

c-Fos 抗体 (E-8) Alexa Fluor® 594

sc-166940 AF594
200 µg/ml
$357.00

c-Fos 抗体 (E-8) Alexa Fluor® 647

sc-166940 AF647
200 µg/ml
$357.00

c-Fos 抗体 (E-8) Alexa Fluor® 680

sc-166940 AF680
200 µg/ml
$357.00

c-Fos 抗体 (E-8) Alexa Fluor® 790

sc-166940 AF790
200 µg/ml
$357.00

c-Fos (E-8) 中和勝肽

sc-166940 P
100 µg/0.5 ml
$68.00

Has E-8 already been tested for the iDisco protocol? If so, at what dilution? Many thanks!

Asked by: Vimast
Thank you for your question. sc-166940 c-Fos (E-8) has not been used in a iDisco protocol.
Answered by: BlakeJ
Date published: 2025-04-01

What dilution do you recommend starting with for flow cytometry using the fluorophore-conjugated version of this antibody? Are there any publications using this antibody for flow cytometry?

Asked by: dr9004
Thank you for your question. We do not have any publications but we do warranty the antibody for flow cytometry. We recommend using 1 µg normal immunoglobulins per 1 million cells. Here is our protocol: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2023-09-19

sc-166940这只c-fos的抗体，我染小鼠脑组织的冰冻切片，0.3%Triton打孔10分钟，山羊血清室温封闭一小时，一抗4℃过夜，使用Alexa Fluo 488的二抗室温避光孵育1小时，但无阳性信号出现，请问问题出在哪里了呢？

Asked by: pangpangmao
感谢您的提问。我们很遗憾地获悉，这种抗体没有达到您的预期。我们希望你的研究取得成功。请联系我们的技术支持部门，亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Answered by: SCBT Representative
Date published: 2022-07-07

What temperature is suitable for storing and reusing c-fos antibody (Anti-Fos/c-Fos Antibody (E-8) X) following dilution, 4C, or -20C freezer?

Asked by: Parisa
We do not recommend freezing our antibodies. Recommended storage is 4C.
Answered by: Technical Service
Date published: 2022-04-07

I have a question about Anti-Fos/c-Fos Antibody (E-8) X (catalog: sc-166940 X) for western blot analysis. I would like to know if it is possible to use both HRP and fluorescent dye conjugated secondary antibodies for detecting the sc-166940 X antibody.

Asked by: Parisa
Thank you for your question. This primary antibody can be detected by either fluorescence-, or by an HRP-based secondary detection. Our secondary binding proteins m-IgG1 BP-HRP: sc-525408 and m-IgG1 BP-CFL 488: sc-533661 have been optimised for detecting primary mouse IgG1 antibodies including this one.
Answered by: Tech Support Europe
Date published: 2022-02-17

Hello, The c-Fos Antibody (K-25): sc-253 was discontinued and replaced by c-Fos Antibody (E-8): sc-166940? In what way are they the same? sc-253 has been shown to work for fish c-fos detection, so will sc-166940 work in the same way? Cheers!

Asked by: Yatz2020
Thank you for your question. c-Fos Antibody (E-8): sc-166940 is a mouse monoclonal IgG1 antibody that was raised against the same peptide antigen that was used to develop the rabbit polyclonal antibody, c-Fos Antibody (K-25): sc-253. The mouse monoclonal antibody has also been published in zebrafish samples, and is recommended to work for zebrafish. For some examples of literature using this mouse monoclonal antibody in zebrafish, please refer on our website to the product citations with PMID's: 30222997, 29626809 and 29061647.
Answered by: Technical Support
Date published: 2020-03-12

Is the c-Fos Antibody (E-8): sc-166940 tested for flow cytometry or if you could suggest any published references that have used this antibody for flow cytometry? Thanks!!

Asked by: Hema
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-08-20

I would like to determine the c-Fos expression in the mouse whole brain cleared with CUBIC or iDISCO. Have you tried to use E-8 for that?

Asked by: Atsu
Thank you for your question. We have not tested this antibody in whole brain tissues, or using either CUBIC or iDISCO.
Answered by: Technical Support
Date published: 2019-07-30
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Rated 1 out of 5 by from Doesn't workWe could not detect any signal on PFA fixated mouse brain slice after IHC
Date published: 2024-04-05
Rated 4 out of 5 by from c-fosGood antibody for detecting c-fos in mouse mice tissue. Dilution: 1:100, perfect staining.
Date published: 2024-01-16
Rated 1 out of 5 by from Did not work in mouse brainNo staining was detected in frozen mouse brain slices fixed in PFA
Date published: 2023-01-05
Rated 1 out of 5 by from Does not work for ChIP in mouse neuronsI tested this antibody for ChIP in mouse primary neurons after stimulation, and there was no detectable enrichment by qPCR. I had used the same protocol for Egr1 ChIP (a similar inducible transcription factor) and it works nicely. Also, quite some background in WB.
Date published: 2022-03-09
Rated 5 out of 5 by from It can be usedIt can be used for osteoclasts which obtained from mouse.
Date published: 2021-07-21
Rated 5 out of 5 by from 条带清晰，效价高之前用的A牌的抗体，就是不出，然后就申请了santa cruz的，想试试，没想到出图效果特别好，条带清晰，效价高
Date published: 2021-07-09
Rated 1 out of 5 by from Per immunofluorescenza non funziona!Ho provato questo anticorpo in immunofluorescenza su spinal cord di topo criostatato (diluizione 1:100), purtroppo senza alcun risultato positivo. Proverò ad utilizzarlo anche per wester blot
Date published: 2020-11-03
Rated 5 out of 5 by from Amazing in CLARITYI use this antibody in CLARITY and it has consistently produced the best images I have ever taken.
Date published: 2020-03-10
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c-Fos 抗体 (E-8) is rated 4.0 out of 5 by 47.
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