Date published: 2025-9-6
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C/EBP beta Antibody (H-7) - Immunofluorescence - Image 134408
C/EBP b (H-7): sc-7962. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.
C/EBP b (H-7): sc-7962. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.

C/EBP beta 抗体 (H-7): sc-7962

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说明书
  • C/EBP beta 抗体 H-7 是小鼠单克隆 IgG2a κ，C/EBP beta抗体, 在276篇文献中引用，规格为200 µg/ml
  • 免疫human物种的C/EBP β的氨基酸199-345
  • C/EBP beta 抗体 (H-7) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 C/EBP β
  • 抗C/EBP beta抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7962 X, 200 µg/0.1 ml)
  • 关于如何获取C/EBP beta (H-7): sc-7962的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是C/EBP beta Antibody (H-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与C/EBP beta Antibody (H-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外C/EBP抗体，包括C/EBP α, C/EBP β, C/EBP δ, C/EBP ε和C/EBP γ

C/EBP β 抗体（H-7）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类来源的 C/EBP β 蛋白。C/EBP β 抗体（H-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。CCAAT增强子结合蛋白（C/EBP）是重要的基本区/亮氨酸拉链（bZIP）转录因子，在肝脏、脂肪组织、血细胞和内分泌胰腺的分化过程中发挥着关键作用。C/EBP β（H-7）单克隆抗体以检测参与调节肝细胞急性期反应的蛋白质而闻名，急性期反应是人体对炎症和感染作出反应的重要过程。C/EBP β基因编码多种异构体，由于在多个AUG起始位点存在可替代的翻译起始，这些异构体含有截短的转录激活结构域，从而在细胞反应中产生多种功能结果。C/EBP β被确认为白细胞介素6依赖性DNA结合蛋白（IL6DBP）、肝脏激活蛋白（LAP）或肝脏富集转录激活蛋白转录因子5（TCF5）。C/EBP β的磷酸化对于DNA结合能力至关重要，在急性期反应中，C/EBP β同源物的结合增加是通过上调和结构修饰实现的，这凸显了翻译后修饰在调节生理变化引起的活性和功能方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

C/EBP beta 抗体 (H-7) 参考文献:

  1. C/EBPbeta 转录因子的两种异构体参与了大鼠高铁血红蛋白基因的急性期调控。  |  Grigorov, I., et al. 1998. Cell Biol Int. 22: 685-93. PMID: 10452839
  2. 白细胞介素-6诱导的STAT3与LAP/C/EBPbeta启动子的系联提示了STAT3转录调控的新机制。  |  Niehof, M., et al. 2001. J Biol Chem. 276: 9016-27. PMID: 11114305
  3. 通过在多个 AUG 起始位点的替代翻译起始调节 CCAAT/增强子结合蛋白-beta 同工酶的合成。  |  Xiong, W., et al. 2001. Nucleic Acids Res. 29: 3087-98. PMID: 11452034
  4. 神经营养素/Trk 受体信号介导 C/EBPalpha, -beta 和 NeuroD 招募到神经元细胞的即刻早期基因启动子，并需要 C/EBPs 来诱导即刻早期基因转录。  |  Calella, AM., et al. 2007. Neural Dev. 2: 4. PMID: 17254333
  5. 磷酸化和 S-S 键诱导的二聚化对 C/EBPbeta 的 DNA 结合和转录激活的影响。  |  Kim, JW., et al. 2007. Proc Natl Acad Sci U S A. 104: 1800-4. PMID: 17264204
  6. Ets-2和C/EBP-beta是滋养层中绵羊滋养层Kunitz结构域蛋白-1基因表达的重要介导因子。  |  Chakrabarty, A. and Roberts, MR. 2007. BMC Mol Biol. 8: 14. PMID: 17326832
  7. 染色质和组蛋白去乙酰化参与 SV40 T 抗原转录调控  |  Valls, E., et al. 2007. Nucleic Acids Res. 35: 1958-68. PMID: 17341466
  8. 体外和体内角质形成细胞分化过程中表达的转录因子 C/EBP alpha, C/EBP beta 和 CHOP (Gadd153)。  |  Maytin, EV. and Habener, JF. 1998. J Invest Dermatol. 110: 238-46. PMID: 9506442
  9. 年龄对对照组和经 LPS 处理的肝脏中 CCAAT/enhancer 结合蛋白 alpha 和 CCAAT/enhancer 结合蛋白 beta 同工酶合成的转录后调控的影响。  |  Hsieh, CC., et al. 1998. Mol Biol Cell. 9: 1479-94. PMID: 9614188

订购信息

产品名称产品编号规格价格数量收藏夹

C/EBP beta 抗体 (H-7)

sc-7962
200 µg/ml
$316.00

C/EBP beta (H-7): m-IgG Fc BP-HRP 套装

sc-528187
200 µg Ab; 10 µg BP
$354.00

C/EBP beta (H-7): m-IgGκ BP-HRP 套装

sc-520509
200 µg Ab, 40 µg BP
$354.00

C/EBP beta (H-7): m-IgG2a BP-HRP 套装

sc-547041
200 µg Ab; 10 µg BP
$354.00

C/EBP beta 抗体 (H-7) X

sc-7962 X
200 µg/0.1 ml
$316.00

C/EBP beta 抗体 (H-7) AC

sc-7962 AC
500 µg/ml, 25% agarose
$416.00

C/EBP beta 抗体 (H-7) HRP

sc-7962 HRP
200 µg/ml
$316.00

C/EBP beta 抗体 (H-7) FITC

sc-7962 FITC
200 µg/ml
$330.00

C/EBP beta 抗体 (H-7) PE

sc-7962 PE
200 µg/ml
$343.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 488

sc-7962 AF488
200 µg/ml
$357.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 546

sc-7962 AF546
200 µg/ml
$357.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 594

sc-7962 AF594
200 µg/ml
$357.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 647

sc-7962 AF647
200 µg/ml
$357.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 680

sc-7962 AF680
200 µg/ml
$357.00

C/EBP beta 抗体 (H-7) Alexa Fluor® 790

sc-7962 AF790
200 µg/ml
$357.00

Hello I want to do Chip-seq with this how much antibody should I use for that?

Asked by: Sebastian
Thank you for your question. We recommended starting with 1ul.
Answered by: BlakeJ
Date published: 2024-11-22

Hello, I used this product and observed double bands in size about 25kDa and 55kDa in western blot. My sample was mouse brain lysate. Is this an appropriate results? I can't found any band around 45kDa... I don't know what's the matter.

Asked by: jiwon
Thank you for your inquiry. Please contact our Asian Technical Service Department for support. We are available at asia@scbio.cn, or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-10-18

Hello, is a mouse-on-mouse blocking kit recommended for usage of this antibody in an immunofluorescence study in mouse tissue?

Asked by: SunnyL
For protocol inquiries on IF and other assays Please contact Technical service
Answered by: Tech Sales
Date published: 2021-05-12

Hello, I was wondering if we could get a test sample of sc-7962 AF488 or a discount on our first order. We have spent hundreds of euro on antibodies that do not work for FC and IHC and would like to avoid this. We would be happy to share good data.

Asked by: EMLyras
Thank you for your question. We do not provide conjugated antibodies as samples, unfortunately. We offer a 12-month warranty on any application an antibody is recommended for, so let us know if any of the antibodies we have provided do not work despite their recommendation.
Answered by: Tech Support Europe
Date published: 2020-08-25

Is this product (sc-7962) the whole antibody or just the kappa light chain?

Asked by: bender
Thank you for your question. This is a regular mouse monoclonal antibody, complete with two kappa light chains and two heavy chains.
Answered by: Tech Service
Date published: 2020-02-19

Your product application says the antibody although raised in mice can be used for IHC in mice. I would like to use this antibody for immunohistochemistry in mouse hippocampal brain tissue but worried I might face challenges with high background.

Asked by: Rachael Dangarembizi
Thank you for your question. This antibody, sc-7962, has been validated and published in mouse samples for IF and IHC. My recommendation is to make sure you block thoroughly and optimize your primary and secondary concentrations, but you should be perfectly fine doing IHC on mouse samples with this antibody.
Answered by: Tech Service
Date published: 2019-08-14

si può usare questo anticorpo per ChIP-qPCR. Se si, quanto? Mi potete giare delle referenze? grazie

Asked by: Blerta
Grazie per la tua domanda. Offriamo una versione TransCruz di questo anticorpo per l'uso in Gel Supershift e ChIP (sc-7962 X, 200 µg / 0,1 ml). Referenze per ChIP sono le pubblicazioni PMID 30693272, PMID 29545928, PMID 21562223. "Can this product be used for ChIP?" Thank you for your question. We offer a TransCruz version of this antibody for use in gel supershift assays and ChIP applications (sc-7962 X, 200 µg / 0,1 ml). References in which this antibody has been used for ChIP assays can be found in the following publications: PMID 30693272, PMID 29545928, PMID 21562223.
Answered by: Tech Support Europe
Date published: 2019-08-06

This antibody can we use in case of bovine ? It shows reactivity only towards mouse and human. Please clear this.

Asked by: Shalu
Thank you for your question. C/EBP β Antibody (H-7): sc-7962 is only recommended for detection of C/EBP β of mouse, rat and human origin by WB, IP, IF and IHC(P). We currently have no internal or published data indicating that the antibody also reacts with bovine cells or tissues. If you want to try out the antibody regardless, please contact our technical service to receive a sample of this antibody.
Answered by: Tech Support Europe
Date published: 2019-07-01
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Rated 5 out of 5 by from C/EBP beta Ab works well for ChiP-qPCR in humanC/EBP beta antibody works well for ChiP-qPCR in human cell lines https://www.pnas.org/doi/10.1073/pnas.1602886113
Date published: 2025-02-16
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2023-04-14
Rated 3 out of 5 by from 条带位置很疑惑我2周前买的抗体，在鼠脑标本 WB，条带在70KD有一个很强的信号，40-55之间有一个很弱的信号，说明书时45KD ，不知道具体条带位置？
Date published: 2022-04-16
Rated 4 out of 5 by from great!I received the sample of this product before and used it recently. The other company's product didn't show up clearly, but this one did, so I'm planning to purchase it.
Date published: 2021-08-26
Rated 5 out of 5 by from Works well for western blottingWorks well for WB, probed at 1/200x dilution for 2 hours.
Date published: 2020-09-29
Rated 5 out of 5 by from CEBPB-alexafluor647Worked really well for flow cytometry analysis in fixed/permeabilized cells. I used 0.2 micrograms of antibody per staining reaction.
Date published: 2019-01-31
Rated 5 out of 5 by from great antibodyIt worked very well on mouse heart samples and very good for IC on cells as well.
Date published: 2017-12-14
Rated 5 out of 5 by from Western blotThis antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dliute 1:1000)
Date published: 2017-11-14
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C/EBP beta 抗体 (H-7) is rated 4.6 out of 5 by 38.
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